Articles
SIMPLE AND RAPID DETECTION OF ODONTOGLOSSUM RINGSPOT VIRUS FROM ORCHIDS BY GELATIN PARTICLE AGGLUTINATION TEST
Article number
432_16
Pages
130 – 131
Language
Abstract
The sensitivity and potential application of gelatin particle agglutination test(GPAT) for detecting odontoglossum ringspot virus(ORSV) in orchids were determined.
The gelatin particles(3 um in diameter) were coated with the anti-ORSV IgG at a concentration of 20 ug/ml and reacted with several different concentrations of purified ORSV as well as varying dilutions of orchid leaf sap extracts.
The GPAT detected as little as 0.1 ug/ml of ORSV when purified virus preparations were used.
In crude leaf sap extracts from Cymbidium plants, the detectable dilution end point for the ORSV by GPAT was 10-4. The optimum dilution of leaf sap extract for the reliable detection of ORSV in Cymbidium and Cattleya plants by GPAT, however, was between 1:100 and 1:800. The GPAT required less than 2 hrs to detect ORSV in orchids.
The GPAT was found to be a rapid, sensitive, reliable and simple serological method for detecting ORSV in orchids.
The gelatin particles(3 um in diameter) were coated with the anti-ORSV IgG at a concentration of 20 ug/ml and reacted with several different concentrations of purified ORSV as well as varying dilutions of orchid leaf sap extracts.
The GPAT detected as little as 0.1 ug/ml of ORSV when purified virus preparations were used.
In crude leaf sap extracts from Cymbidium plants, the detectable dilution end point for the ORSV by GPAT was 10-4. The optimum dilution of leaf sap extract for the reliable detection of ORSV in Cymbidium and Cattleya plants by GPAT, however, was between 1:100 and 1:800. The GPAT required less than 2 hrs to detect ORSV in orchids.
The GPAT was found to be a rapid, sensitive, reliable and simple serological method for detecting ORSV in orchids.
Authors
Y.J. LA, J.O. Park, H.W. Jung
Keywords
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