Articles
CYTOPATHOLOGICAL EVIDENCE FOR THE PROCESS OF TOMATO SPOTTED WILT VIRUS PARTICLE PRODUCTION AND FORMATION OF VIRUS INCLUSIONS
Article number
432_46
Pages
376 – 383
Language
Abstract
Ultrastructural changes were investigated in leaf parenchyma cells of Nicotiana rustica 9 and 15 days after inoculation with TSWV. Nine days after inoculation the virus induced some changes in the structure of the Golgi apparatus.
The space between particular cisterns increased and the marginal parts of the cisterns became curved.
Some dark substance appeared on the surface of the cisterns.
In this way some closed areas were formed surrounded with the dictyosome cistern membranes.
The division of such regions into smaller units was observed.
On the 9th day after inoculation some single membrane-bound TSWV particles were observed.
Such one-particle sacs were characterized by a narrow space between the external particle envelope and the membrane surrounding the bundle.
In the neighbourhood of those single particles some specific membranous structures were observed composed of tubular branched cisterns with ribosomes.
Also some fusions were observed between sacs containing a single particle or from sacs with cisterns of the rough and smooth endoplasmatic reticulum.
The space between particular cisterns increased and the marginal parts of the cisterns became curved.
Some dark substance appeared on the surface of the cisterns.
In this way some closed areas were formed surrounded with the dictyosome cistern membranes.
The division of such regions into smaller units was observed.
On the 9th day after inoculation some single membrane-bound TSWV particles were observed.
Such one-particle sacs were characterized by a narrow space between the external particle envelope and the membrane surrounding the bundle.
In the neighbourhood of those single particles some specific membranous structures were observed composed of tubular branched cisterns with ribosomes.
Also some fusions were observed between sacs containing a single particle or from sacs with cisterns of the rough and smooth endoplasmatic reticulum.
Fifteen days after inoculation large extensive inclusions of virus particles could be observe.
These arose from the sacs containing a few TSWV particles.
At that time there were no single particles.
Golgi apparatus with regularly distributed cisterns were present at the margin of the virus particle inclusions.
Amorphic and fibrous inclusions were not observed in the earlier periods after inoculation, but appeared in the later periods.
Authors
A. Rudzinska-Langwald, M. Kaminska
Keywords
TSWV, tospovirus, cytopathology
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