Articles
IDENTIFICATION OF PHYTOPLASMAS IN ALSTROEMERIA
Article number
432_38
Pages
312 – 319
Language
Abstract
During a survey of viruses infecting alstroemeria, plants showing virescent and malformed flowers were found under greenhouse conditions.
After DNA extraction 17 samples were tested with direct and nested-PCR using several phytoplasma 16S ribosomal primer pairs.
In five samples the presence of a 1.2 kb DNA product identical to those observed in phytoplasma strain control DNAs was detected.
No amplification was observed in the other alstroemeria samples and in negative controls.
Four of the positive samples were from plants with green and malformed flowers and one was from an asymptomatic plant.
The micropropagated shoots tested were also positive to phytoplasma presence.
Nested-PCR with group specific primers and RFLP analyses identified the phytoplasmas to belong to the aster yellows group (16SrI) subgroups 16SrI-B, I-C and I-G and to the peach X disease group (16SrIII).
After DNA extraction 17 samples were tested with direct and nested-PCR using several phytoplasma 16S ribosomal primer pairs.
In five samples the presence of a 1.2 kb DNA product identical to those observed in phytoplasma strain control DNAs was detected.
No amplification was observed in the other alstroemeria samples and in negative controls.
Four of the positive samples were from plants with green and malformed flowers and one was from an asymptomatic plant.
The micropropagated shoots tested were also positive to phytoplasma presence.
Nested-PCR with group specific primers and RFLP analyses identified the phytoplasmas to belong to the aster yellows group (16SrI) subgroups 16SrI-B, I-C and I-G and to the peach X disease group (16SrIII).
Authors
A. Bertaccini, M. Vibio, M.G. Bellardi, A. Danielli
Keywords
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