Articles
DETECTION OF TOMATO SPOTTED WILT VIRUS IN DAHLIA
Article number
432_47
Pages
384 – 391
Language
Abstract
Quality inspection of Dutch dahlias includes visual inspection on viruses in the field.
Tomato spotted wilt virus (TSWV) is known to occur in Dutch dahlias for many decades, causing concentric ring patterns and yellowish, dark green or necrotic spots on the leaves.
Detection by ELISA since 1992 revealed latent infections of TSWV throughout most of the cultivars.
As leaf tests proved unreliable due to unequal distribution of TSWV in dahlia plants, tuber tests were introduced in the inspection scheme of the Flowerbulb Inspection Service.
After a minimum storage period of four weeks, mixed samples of three roots per tuber allow ELISA detection of TSWV with sufficient reliability.
When sampling tubers meant for the production of cuttings, both roots and corresponding tubers may be labeled.
This option allows growers to remove infected tubers from the stocks using the ELISA results from the samples.
This unique combination of inspection and selection, helped by low reinfection, led to a rapid decline of TSWV in Dutch dahlias.
Tomato spotted wilt virus (TSWV) is known to occur in Dutch dahlias for many decades, causing concentric ring patterns and yellowish, dark green or necrotic spots on the leaves.
Detection by ELISA since 1992 revealed latent infections of TSWV throughout most of the cultivars.
As leaf tests proved unreliable due to unequal distribution of TSWV in dahlia plants, tuber tests were introduced in the inspection scheme of the Flowerbulb Inspection Service.
After a minimum storage period of four weeks, mixed samples of three roots per tuber allow ELISA detection of TSWV with sufficient reliability.
When sampling tubers meant for the production of cuttings, both roots and corresponding tubers may be labeled.
This option allows growers to remove infected tubers from the stocks using the ELISA results from the samples.
This unique combination of inspection and selection, helped by low reinfection, led to a rapid decline of TSWV in Dutch dahlias.
Authors
A.R. van Schadewijk
Keywords
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