Articles
APPLICATIONS OF BIOTECHNOLOGY TO KIWIFRUIT (ACTINIDIA) IN NEW ZEALAND
Article number
461_19
Pages
193 – 198
Language
Abstract
A comprehensive traditional breeding programme in the genus Actinidia is being augmented by the application of molecular and transformation technology.
DNA fingerprinting of cultivars and breeding stock using RAPDs and microsatellites has been used for quality assurance in the breeding programme.
Molecular markers linked to sex have been obtained by bulk segregant analysis of a sibling population using RAPDs, and further development of a male-linked marker into a SCAR (sequence characterised amplified region) has permitted its use in screening seedling populations.
Investigations into the genetic basis of dioecy by differential screening of cDNA libraries made from floral buds during development has allowed the isolation and characterisation of differential clones active during gametophytic development. Agrobacterium-mediated transformation systems have been applied to several species, and plants carrying marker genes have been obtained.
An on-going programme is investigating genes controlling characters of possible interest to breeders.
DNA fingerprinting of cultivars and breeding stock using RAPDs and microsatellites has been used for quality assurance in the breeding programme.
Molecular markers linked to sex have been obtained by bulk segregant analysis of a sibling population using RAPDs, and further development of a male-linked marker into a SCAR (sequence characterised amplified region) has permitted its use in screening seedling populations.
Investigations into the genetic basis of dioecy by differential screening of cDNA libraries made from floral buds during development has allowed the isolation and characterisation of differential clones active during gametophytic development. Agrobacterium-mediated transformation systems have been applied to several species, and plants carrying marker genes have been obtained.
An on-going programme is investigating genes controlling characters of possible interest to breeders.
Authors
C.F. Harvey, L.G. Fraser, G.P. Gill, R.W.M. Fung, M. Yoon, K. Weising, R.C. Gardner
Keywords
transformation, DNA fingerprinting, DNA markers, dioecy
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