Articles
CO-TRANSFORMATION AS A POTENTIAL TOOL TO CREATE MULTIPLE AND DURABLE DISEASE RESISTANCE IN BANANA (MUSA SPP.)
Article number
461_41
Pages
361 – 366
Language
Abstract
Recent progress with more advanced in vitro cultures of banana such as establishment of regenerable embryogenic cell suspension (ECS) opened the opportunity for the production of transgenic bananas.
For that purpose, foreign gene introduction by particle bombardment to ECSs proved to be the most suitable technique in combination with selection in the presence of various antibiotics.
Several hundred independent transgenic plants have been regenerated so far from the plantain cultivar Three Hand Planty, the cooking banana Bluggoe and the Cavendish dessert banana cultivar Williams.
Presence of the introduced genes has been confirmed by large-scale PCR screening and integrative transformation has been demonstrated by Southern gel blot hybridisation.
In addition, bombardment of combinations of unlinked and linked chimaeric genes has resulted in high co-transformation frequencies which may allow for the introduction of complex traits to banana.
Applications of this transformation technology to evaluate different strategies for creating resistance to fungal and viral pathogens in banana are also discussed.
For that purpose, foreign gene introduction by particle bombardment to ECSs proved to be the most suitable technique in combination with selection in the presence of various antibiotics.
Several hundred independent transgenic plants have been regenerated so far from the plantain cultivar Three Hand Planty, the cooking banana Bluggoe and the Cavendish dessert banana cultivar Williams.
Presence of the introduced genes has been confirmed by large-scale PCR screening and integrative transformation has been demonstrated by Southern gel blot hybridisation.
In addition, bombardment of combinations of unlinked and linked chimaeric genes has resulted in high co-transformation frequencies which may allow for the introduction of complex traits to banana.
Applications of this transformation technology to evaluate different strategies for creating resistance to fungal and viral pathogens in banana are also discussed.
Authors
S. Remy, I. François, B.PA. Cammue, R. Swennen, L. Sági
Keywords
cell suspension, particle bombardment, transgenic plant
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