Articles
ENGINEERING FOR RESISTANCE TO SCMV IN SUGARCANE
Article number
461_44
Pages
385 – 392
Language
Abstract
Sugarcane mosaic potyvirus (SCMV) is an important pathogen of sugarcane (Saccharum L. interspecific hybrids) and can cause significant yield losses in susceptible cultivars.
Pathogen-derived resistance using the viral coat protein (CP) gene has been successfully demonstrated in other host/virus combinations and is being evaluated in sugarcane.
Sugarcane plants were cotransformed with the CP gene of sugarcane mosaic virus (SCMV) and the neomycin phosphotransferasell (nptII) (selectable marker) gene by microprojectile bombardment.
These genes were constructed with either the Emu (an artificial promoter) or the ubiquitin (Ubi) promoter.
Over 80 transgenic lines were assessed for nptII activity by dot-blot and ELISA. There was considerable variability between lines in npt expression, with the most active Ubi line producing npt enzyme to a level equivalent to 0.03% of total soluble protein.
Initial results show no correlation between the number of copies of the transgene and the level of npt expression.
Plants containing the CP transgene have been selected, propagated and challenged with SCMV in glasshouse trials.
Ten lines were resistant to challenge inoculation with the virus and these lines are being further characterised.
Pathogen-derived resistance using the viral coat protein (CP) gene has been successfully demonstrated in other host/virus combinations and is being evaluated in sugarcane.
Sugarcane plants were cotransformed with the CP gene of sugarcane mosaic virus (SCMV) and the neomycin phosphotransferasell (nptII) (selectable marker) gene by microprojectile bombardment.
These genes were constructed with either the Emu (an artificial promoter) or the ubiquitin (Ubi) promoter.
Over 80 transgenic lines were assessed for nptII activity by dot-blot and ELISA. There was considerable variability between lines in npt expression, with the most active Ubi line producing npt enzyme to a level equivalent to 0.03% of total soluble protein.
Initial results show no correlation between the number of copies of the transgene and the level of npt expression.
Plants containing the CP transgene have been selected, propagated and challenged with SCMV in glasshouse trials.
Ten lines were resistant to challenge inoculation with the virus and these lines are being further characterised.
Authors
P.A. Joyce, R.B. McQualter, M.J. Bernard, G.R. Smith
Keywords
transgenes, mosaic, potyvirus, resistance, sugarcane, Saccharum L., SCMV
Online Articles (60)