Articles
THE EFFECT OF EXOGENOUS POLYAMINES ON SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM SORGHUM BICOLOR AND SACCHARUM SPP.
Article number
461_53
Pages
451 – 458
Language
Abstract
Plant regeneration was significantly increased from somatic embryogenic callus of sorghum (Sorghum bicolor [L.] Moench) and sugarcane (Saccharum spp. hybrids) following induction on media supplemented with either putrescine (0.1 to 10 mM), spermine (0.1 to 1.0 μM) or spermidine (0.1 to 1.0 μM). Meanwhile, ethylene biosynthesis significantly decreased and the percentage of explants that underwent embryogenesis increased in response to the addition of polyamines.
All responses to the polyamines were genotype specific, with the high phenolic-producing lines significantly benefiting from these additions to the medium.
Embryogenic callus induction occurred over the 28 days of culture for sorghum and 56 days for sugarcane.
This was then followed by the selection and transfer of callus to polyamine-free regeneration medium.
Most notable was the significant increase in plant regeneration from the highly recalcitrant sorghum genotype TAM422 and the sugarcane cultivars Q96 and Q117, all of which produced high levels of phenolics throughout the period of in vitro culture.
All responses to the polyamines were genotype specific, with the high phenolic-producing lines significantly benefiting from these additions to the medium.
Embryogenic callus induction occurred over the 28 days of culture for sorghum and 56 days for sugarcane.
This was then followed by the selection and transfer of callus to polyamine-free regeneration medium.
Most notable was the significant increase in plant regeneration from the highly recalcitrant sorghum genotype TAM422 and the sugarcane cultivars Q96 and Q117, all of which produced high levels of phenolics throughout the period of in vitro culture.
Authors
H.R. Sargent, I.D. Godwin, S.W. Adkins
Keywords
polyamines, somatic embryogenesis, Sorghum bicolor (L.) Moench, sugarcane
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