Articles
GREENHOUSE AND FIELD EVALUATIONS OF COAT-PROTEIN TRANSGENIC PAPAYA RESISTANT TO PAPAYA RINGSPOT VIRUS
Article number
461_36
Pages
321 – 328
Language
Abstract
The coat protein (CP) gene of a Taiwan strain of papaya ringspot virus (PRSV) was efficiently transferred to papaya by Agrobacterium-mediated transformation, assisted by wounding of embryogenic tissues derived from immature zygotic embryos with carborundum in liquid phase.
Thirty-eight transgenic lines were micropropagated by tissue culture and their resistance to PRSV was evaluated by mechanical inoculation with the virus under greenhouse conditions.
Among them, 9 lines were found highly resistant to PRSV and 8 lines moderately resistant.
The highly resistant lines also conferred broad-spectrum resistance to PRSV strains from Thailand and Hawaii.
Three lines of transgenic papaya, 16–0–1, 17–0–1 and 17–0–5, were further evaluated under field conditions in two different locations, the first started from January 1996 and the second from August 1996. In the first trial, only 0, 2, and 0% of lines 16–0–1, 17–0–1 and 17–0–5 (each with 100 plants tested), respectively, were infected by PRSV 12 months after planting, while the control plants were 100% infected eight months after planting.
The transgenic lines in the second trial, which were under a severe challenge pressure, had infection rates of 10–20% five months after planting, while the control was completely infected three months after planting.
The preliminary results of the field tests indicated that the CP transgenic papaya lines have a great potential for control of PRSV in Taiwan.
Thirty-eight transgenic lines were micropropagated by tissue culture and their resistance to PRSV was evaluated by mechanical inoculation with the virus under greenhouse conditions.
Among them, 9 lines were found highly resistant to PRSV and 8 lines moderately resistant.
The highly resistant lines also conferred broad-spectrum resistance to PRSV strains from Thailand and Hawaii.
Three lines of transgenic papaya, 16–0–1, 17–0–1 and 17–0–5, were further evaluated under field conditions in two different locations, the first started from January 1996 and the second from August 1996. In the first trial, only 0, 2, and 0% of lines 16–0–1, 17–0–1 and 17–0–5 (each with 100 plants tested), respectively, were infected by PRSV 12 months after planting, while the control plants were 100% infected eight months after planting.
The transgenic lines in the second trial, which were under a severe challenge pressure, had infection rates of 10–20% five months after planting, while the control was completely infected three months after planting.
The preliminary results of the field tests indicated that the CP transgenic papaya lines have a great potential for control of PRSV in Taiwan.
Authors
S. Yeh, H. Bau, Y. Cheng, T. Yu, J. Yang
Keywords
Virus control, Field test
Online Articles (60)