Articles
SCREENING MELONS FOR RESISTANCE TO GUMMY STEM BLIGHT
Article number
510_28
Pages
171 – 178
Language
Abstract
Seedling screens of melon (Cucumis melo) for high resistance to gummy stem blight, caused by the fungus Didymella bryoniae (Auersw.) Rehm, were conducted on 180 accessions of C. melo var. reticulatus, var. cantaloupensis, var. inodorus, var. makuwa, var. conomon, var. momordica, var. agrestis (weedy type melon), and other types.
Seedlings were inoculated with three isolates of D. bryoniae using a petiole-mediated inoculation method.
Subsequently, disease damage was evaluated based on stem symptoms.
In order to identify accessions having high resistance, a three-step screening system (initial screen, secondary screen with two other D. bryoniae isolates, and then confirming screen with more severe temperature conditions) was employed.
Through this screening system, MEX-2 was confirmed as the accession having the highest resistance to the gummy stem blight pathogen.
KOR-33, J-3, and JMu-15 were also regarded as highly resistant.
All of the accessions identified as resistant were C. melo var. agrestis.
Seedlings were inoculated with three isolates of D. bryoniae using a petiole-mediated inoculation method.
Subsequently, disease damage was evaluated based on stem symptoms.
In order to identify accessions having high resistance, a three-step screening system (initial screen, secondary screen with two other D. bryoniae isolates, and then confirming screen with more severe temperature conditions) was employed.
Through this screening system, MEX-2 was confirmed as the accession having the highest resistance to the gummy stem blight pathogen.
KOR-33, J-3, and JMu-15 were also regarded as highly resistant.
All of the accessions identified as resistant were C. melo var. agrestis.
Authors
Y. Sakata, T. Wako, M. Sugiyama, M. Morishita
Keywords
Cucumis melo, Didymella bryoniae, disease resistance, screening
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