Home > VII Eucarpia Meeting on Cucurbit Genetics and Breeding > ISOLATION OF MESOPHYLL PROTOPLASTS OF CUCUMIS SPP. AND CUCURBITA SPP.

Articles

ISOLATION OF MESOPHYLL PROTOPLASTS OF CUCUMIS SPP. AND CUCURBITA SPP.

Article number

510_68

Pages

425 – 432

Language

Abstract

Well-developed first or second true leaves, originating from in vitro plants of two Cucumis species (C. melo, C. metuliferus) and three Cucurbita species (C. ficifolia, C. maxima, C. pepo), were used for protoplast isolation.
The optimal composition of the enzyme solution and the most suitable isolation method were studied.
The density and viability of protoplasts were used as efficiency criteria.
The best isolation method and the enzyme solution were more suitable for Cucumis spp. than for Cucurbita spp., as the former had more viable and less damaged (plasmolyzed) protoplasts.

Publication

VII Eucarpia Meeting on Cucurbit Genetics and Breeding

Authors

B. Navrátilová, L. Rokytová, A. Lebeda

Keywords

Cucumber, squash, genetic resources, protoplast yield and viability, enzyme solution, isolation methods

Full text

https://doi.org/10.17660/ActaHortic.2000.510.68

Online Articles (77)

2. DEVELOPMENT OF MELON CULTURE AND BREEDING IN ISRAEL

Z. Karchi

3. WHERE IS SWEDEN? THE INCREASE IN PRODUCTION OF CUCURBITA (SQUASH AND PUMPKINS) OVER THE PAST FEW YEARS

L. Lehmann

4. BREEDING FOR HIGH FRUIT YIELD IN CUCUMBER

N. V. Shetty | Todd C. Wehner

5. SOME COMMENTS ON INFRASPECIFIC CLASSIFICATION OF CULTIVARS OF MELON

M. Pitrat | P. Hanelt | K. Hammer

6. THE ORIGIN OF MELON, CUCUMIS MELO: A REVIEW OF THE LITERATURE

T. Kerje | M. Grum

7. F₁ HYBRID PICKLING CUCUMBERS DEVELOPED FOR INCREASED YIELD, EARLINESS AND RESISTANCE TO DOWNY MILDEW (PSEUDOPERONOSPORA CUBENSIS)

Roch W. Doruchowski | E. Lakowska-Ryk

8. OUTCROSSING RATE AND IMPLICATIONS FOR THE IMPROVEMENT OF A SEGREGATING POPULATION OF WATERMELON

M.A.J.d. Ferreira | R. Vencovsky | M.L.C. Vieira | M.A. de Queiróz

9. INDUCED RESISTANCE TO DOWNY MILDEW AND FUSARIUM WILT IN CUCURBITS

A. Ovadia | R. Biton | Y. Cohen

10. POWDERY MILDEW FIELD INFECTION ON LEAVES AND STEMS OF CUCURBITA PEPO ACCESSIONS

E. Kristková | A. Lebeda

11. VIRULENCE DIFFERENCES BETWEEN TWO SPANISH ISOLATES OF SPHAEROTHECA FULIGINEA RACE 2 ON MELON

J.M. Alvarez | M.L. Gómez-Guillamón | N.A. Torés | I. Cánovas | E. Floris

12. HISTOLOGICAL OBSERVATIONS OF POWDERY MILDEW RESISTANCE IN DIPLOID AND HAPLOID MELONS

M. Kuzuya | K. Hosoya | Y. Hayato Masuya | K. Tomita | H. Ezura

13. SCREENING FIELD RIESENSCHÄEL CUCUMBERS FOR RESISTANCE TO DOWNY MILDEW: MANAGEMENT OF INTERPLOT INTERFERENCE PROBLEMS

Gitte K. Bjoern | H. Kampmann

14. TWO RACES OF PSEUDOPERONOSPORA CUBENSIS ON CUCUMBERS IN BULGARIA

D. Angelov | P. Georgiev | L. Krasteva

15. CROSS-INFECTIVITY OF SPHAEROTHECA FULIGINEA TO WATERMELON, MELON, AND CUCUMBER

Y. Cohen | A. Baider | L. Petrov | L. Sheck | V. Voloisky

16. THE CUCURBIT LEGACY OF ANTOINE NICOLAS DUCHESNE (1747–1827)

Harry S. Paris

17. TROPICAL PUMPKIN HYBRID DEVELOPMENT: LANDRACES TO HYBRID CULTIVARS

R. Bruce Carle | Donald N. Maynard | L. Wessel-Beaver

18. INTERRELATIONS AMONG FRUIT AND SEED CHARACTERISTICS OF OIL PUMPKIN

J. Berenji | D. Papp

19. WATERMELON BREEDING IN BRAZIL

M. Queiroz | R. Dias | F. Souza | M. Ferreira | R. Borges

20. GENETIC ASSESSMENT OF THE UNITED STATES NATIONAL CUCUMBER COLLECTION

Jack E. Staub | V. Ivandic

21. BREEDING OF HULL-LESS SEEDED PUMPKINS (CUCURBITA PEPO) FOR THE USE OF THE OIL

J. Winkler

22. CANONICAL DISCRIMINANT ANALYSIS OF NINE PICKLING CUCUMBER (CUCUMIS SATIVUS) ACCESSIONS

Z. Susic | D. Jelovac | L. Stankovic | J. Zdravkovic

23. SELECTING DOWNY MILDEW-RESISTANT SHORT-FRUITED CUCUMBERS

D. Angelov | L. Krasteva

24. CUCURBITACEAE WEB SITE

Thomas C. Andres

25. VARIOUS APPROACHES TOWARD CONTROLLING SUDDEN WILT OF MELONS IN ISRAEL

26. NARROW-SENSE HERITABILITY ESTIMATES FOR ROOT TRAITS AND MONOSPORASCUS CANNONBALLUS TOLERANCE IN MELON (CUCUMIS MELO) BY PARENT-OFFSPRING REGRESSION

K. Crosby

27. ARTIFICIAL INOCULATION METHODS AND SELECTION CRITERIA FOR BREEDING MELONS AGAINST VINE DECLINE

A. Iglesias | B. Picó | F. Nuez

28. COMPARISON OF LEAF-AREA INDEX WITH ROOT WEIGHT FOR ASSESSING PLANT DAMAGE BY SOIL-BORNE PATHOGENS

M. Biernacki | Benny D. Bruton

29. SCREENING MELONS FOR RESISTANCE TO GUMMY STEM BLIGHT

Y. Sakata | T. Wako | M. Sugiyama | M. Morishita

30. MUSKMELON GROWTH AND YIELD IN RESPONSE TO FUMIGATION

M.E. Miller | R.D. Martyn | B.D. Bruton

31. EPIPHYTIC MICROFLORA ON THE SEEDS OF MELONS AND WATERMELONS

K. Sapundzieva | I. Kuzmanova | L. Krasteva

32. SEARCHING FOR CUCURBITA GERMPLASM: COLLECTING MORE THAN SEEDS

Thomas C. Andres

33. SEGREGATION DISTORTION IN CUCURBITA PEPO

Harry S. Paris

34. RESISTANCE TO DOWNY MILDEW, PSEUDOPERONOSPORA CUBENSIS, IN CUCUMBERS

35. INVESTIGATIONS OF SEX EXPRESSION OF CUCUMIS SATIVUS BY GRAFTING AND COPPER APPLICATION

H. Mibus | I. Vural | T. Tatlioglu

36. INTERACTIONS BETWEEN MORPHOTYPES OF CUCURBITA PEPO AND OBLIGATE BIOTROPHS (PSEUDOPERONOSPORA CUBENSIS, ERYSIPHE CICHORACEARUM AND SPHAEROTHECA FULIGINEA)

A. Lebeda | E. Krístková

37. FRUIT SHAPE, SIZE AND SEED YIELD IN CUCURBITA PEPO

H. Nerson | Harry S. Paris | Effi P. Paris

38. DOES JEWISH LAW PERMIT GRAFTING MELON OR WATERMELON ON PUMPKIN?

M. Kislev

39. MOLECULAR AND PHENOTYPIC VARIATION IN MELON PI 313970

James D. McCreight

40. MORPHOLOGICAL CHARACTERISTICS AND POWDERY MILDEW RESISTANCE OF CUCUMIS MELO LAND RACES IN GREECE

N. Fanourakis | Z. Tsekoura | E. Nanou

41. ORGANIZATION OF MELON PLANT GENETIC RESOURCES IN BULGARIA

L. Krasteva

42. WATERMELON GENETIC RESOURCES IN BULGARIA

L. Krasteva

43. CUCUMBER GENETIC RESOURCES OF LATVIAN ORIGIN

L. Lepse | M. Baumane | I. Rashal

44. COMBINING ABILITY TEST BETWEEN SOME LINES OF CUCUMIS SATIVUS L.

A. Balliu | M. Hallidri

45. ETHEPHON TREATMENT ON A MONOECIOUS CUCUMBER ACCESSION FOR HYBRID SEED PRODUCTION

A. Korzeniewska | T. Galecka | K. Niemirowicz-Szczytt

46. DOMINANT INHERITANCE OF DOWNY MILDEW RESISTANCE IN MELONS

D. Angelov | L. Krasteva

47. A LEAF-DISC BIOASSAY FOR SCREENING CUCUMBERS FOR RESISTANCE TO DOWNY MILDEW

Y. Cohen | L. Petrov | A. Baider

48. RESISTANCE OF CUCURBITA AND LAGENARIA TO THE CARMINE SPIDER MITE, TETRANYCHUS CINNABARINUS (ACARI: TETRANYCHIDAE)

M. Edelstein | Y. Tadmor | F. Mansour

49. INHERITANCE OF SILVERLEAF RESISTANCE IN CUCURBITA MOSCHATA

L. Wessel-Beaver

50. INSECT RESISTANCE IN CUCUMIS MELO ACCESSION 90625

N. Boissot | C. Pavis | R. Guillaume | D. Lafortune | N. Sauvion

51. RESISTANCE TO APHIS GOSSYPII AND TO VIRUS TRANSMISSION BY THIS APHID IN MELON

C. Soria | J.A. Diaz | E. Moriones | M.L. Gómez-Guillamón

52. APHIS GOSSYPII RESISTANCE IN CUCUMIS MELO: THE GENETIC BASIS OF A PHLOEM-SPECIFIC DEFENSE

J. Klingler | I. Kovalski | L. Silberstein | R. Perl-Treves | Gary A. Thompson

53. THE DYNAMICS OF VIRUSES AFFECTING CUCURBITS IN ISRAEL: 40 YEARS SINCE 1960

S. Cohen | R. Ben-Joseph

54. CUCURBIT-POTYVIRUS INTERACTIONS INFLUENCING VIRUS MOVEMENT AND ACCUMULATION

R. Grumet | Z. Ullah | A. Gal-On | B. Raccah

55. CUCUMBER MOSAIC VIRUS MOVEMENT PROTEIN MODIFIES PLASMODESMAL FUNCTION IN TRANSGENIC MELON PLANTS

D. Shalitin | K. Shamay | S. Wolf

56. GENETIC ENGINEERING OF ATTENUATED VIRAL CDNA OF ZUCCHINI YELLOW MOSAIC VIRUS FOR PROTECTION OF CUCURBITS

A. Gal-On | P. Katsir | W. Yongzang

57. CROSS-PROTECTION OF CUCURBITACEAE FROM ZUCCHINI YELLOW MOSAIC POTYVIRUS

58. TOWARDS AN INTEGRATED LINKAGE MAP OF CUCUMBER: MAP MERGING

Jack E. Staub | Felix C. Serquen

59. CONSTRUCTION OF A REFERENCE GENETIC MAP OF MELON

60. THE SPANISH MELON GENOME PROJECT: CONSTRUCTION OF A SATURATED GENETIC MAP

61. PARTIAL CLONING OF P45 FROM RESISTANT MELON REVEALS HIGH HOMOLOGY WITH AMINOTRANSFERASES

D. Taler | D. Kenigsbuch | Y. Cohen

62. ESTIMATION OF GENETIC DIVERSITY AMONG CITRULLUS ACCESSIONS USING RAPD MARKERS

A. Levi | Claude E. Thomas | A. P. Keinath | Todd C. Wehner

63. IDENTIFICATION OF QTLS CONTRIBUTING TO RESISTANCE TO DIFFERENT STRAINS OF CUCUMBER MOSAIC CUCUMOVIRUS IN MELON

64. SEARCH FOR MOLECULAR MARKERS ASSOCIATED WITH RESISTANCE TO VIRUSES IN MELON

Y. Danin-Poleg | G. Tzuri | N. Reis | Z. Karchi | N. Katzir

65. AGROBACTERIUM-MEDIATED TRANSFORMATION IN CUCUMIS SATIVUS VIA DIRECT ORGANOGENESIS

A. Ganapathi | R. Perl-Treves

66. EMBRYO CULTURES OF WILD AND CULTIVATED SPECIES OF THE GENUS CUCUMIS L.

V. Ondrej | B. Navrátilová | A. Lebeda

67. TOWARD THE DEVELOPMENT OF MOLECULAR MARKERS LINKED TO RACE 2 FUSARIUM WILT RESISTANCE IN MELON (CUCUMIS MELO L.)

T. Karsies | R. Dean | C. Thomas

68. POSSIBILITIES OF INDUCING POLYEMBRYONY IN CUCUMBER (CUCUMIS SATIVUS)

P. Nowaczyk | L. Nowaczyk

69. ISOLATION OF MESOPHYLL PROTOPLASTS OF CUCUMIS SPP. AND CUCURBITA SPP.

B. Navrátilová | L. Rokytová | A. Lebeda

70. FURTHER ISSR AND PRELIMINARY SSR ANALYSIS OF RELATIONSHIPS AMONG ACCESSIONS OF CUCURBITA PEPO

71. LINKAGE GROUPS OF CUCUMIS MELO, INCLUDING RESISTANCE GENE HOMOLOGUES AND KNOWN GENES

72. BIOCHEMISTRY OF SUGAR ACCUMULATION IN MELONS AS RELATED TO THE GENETIC IMPROVEMENT OF FRUIT QUALITY

73. AROMA FORMATION IN MUSKMELONS: VOLATILE ACETATES IN RIPENING FRUITS

74. GENES INVOLVED IN ETHYLENE SYNTHESIS AND PERCEPTION IN CUCUMBER

75. THE ROLE OF ETHYLENE IN REGULATING CELL WALL-DEGRADING ENZYME ACTIVITY USING ANTISENSE ACC-OXIDASE IN CANTALOUPE MELONS

R. Botondi | M. Cardarelli | F. Mencarelli

76. THE CONTRIBUTION OF SUCROSE TO TOTAL SUGAR CONTENT IN MELONS

Y. Burger | S. Shen | M. Petreikov | Arthur A. Schaffer

77. SENSORY EVALUATION OF CHARENTAIS-TYPE MELONS: AN EXPLORATORY TOOL

C. Guérineau | E. Denis | D. Scandella | B. Navez | N. Lancelin