Articles
MICROPROPAGATION OF PINEAPPLE GUAVE THROUGH ORGANOGENESIS AND AXILLARY SHOOT PROLIFERATION
Article number
520_11
Pages
109 – 118
Language
Abstract
Feijoa sellowiana Berg (pineapple guava or feijoa) is a woody myrtaceous species from South America, used both as an ornamental and as a fruit crop.
The agronomic interest of this species, and the difficulties in propagating it by conventional methods, led us to test different tissue culture techniques.
The present work reports production of feijoa plants by organogenesis and axillary shoot proliferation.
Adventitious shoots were produced from young leaves.
Highest rates of organogenesis (20% of responding explants and an average of 9.8 shoots per responding explant) were obtained with MS medium containing 1.0 mg.l-1 benzylaminopurine (BA), 001 mg.l-1 naphthaleneacetic acid (NAA) and 500 mg.l-1 casein hydrolysate.
Shoots were also regenerated from zygotic and somatic embryos.
Here, media containing 0.5 mg.l-1 BA gave the best results (43% induction and 4.9 shoots per induced explant). Nodal segments from seedlings or from 2-year-old plants, showed axillary bud break of more than 90% on MS medium with 0.3 mg.l-1 BA. Isolated shoots obtained by organogenesis or axillary shoot proliferation rooted well (65%) after one week on MS medium containing 1% sucrose, the major salts reduced to half strength and 1.0 mg.l-1 indolebutyric acid (IBA) followed by transfer to the same medium, but without hormones.
Histological studies showed that adventitious buds have a peripheral origin whereas root primordia were formed near the vascular bundles.
The agronomic interest of this species, and the difficulties in propagating it by conventional methods, led us to test different tissue culture techniques.
The present work reports production of feijoa plants by organogenesis and axillary shoot proliferation.
Adventitious shoots were produced from young leaves.
Highest rates of organogenesis (20% of responding explants and an average of 9.8 shoots per responding explant) were obtained with MS medium containing 1.0 mg.l-1 benzylaminopurine (BA), 001 mg.l-1 naphthaleneacetic acid (NAA) and 500 mg.l-1 casein hydrolysate.
Shoots were also regenerated from zygotic and somatic embryos.
Here, media containing 0.5 mg.l-1 BA gave the best results (43% induction and 4.9 shoots per induced explant). Nodal segments from seedlings or from 2-year-old plants, showed axillary bud break of more than 90% on MS medium with 0.3 mg.l-1 BA. Isolated shoots obtained by organogenesis or axillary shoot proliferation rooted well (65%) after one week on MS medium containing 1% sucrose, the major salts reduced to half strength and 1.0 mg.l-1 indolebutyric acid (IBA) followed by transfer to the same medium, but without hormones.
Histological studies showed that adventitious buds have a peripheral origin whereas root primordia were formed near the vascular bundles.
Authors
J.M. Canhoto, G.S. Gruz
Keywords
Feijoa sellowiana, organogenesis, pineapple guave, rooting, shoot proliferation
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