Articles
IN VITRO PROPAGATION OF SKIMMIA (SKIMMIA JAPONICA THUNB.)
Article number
520_8
Pages
91 – 96
Language
Abstract
Shoot tip and lateral bud explants of female and male Skimmia japonica clones were established in vitro in Murashige and Skoog salt medium.
High proliferation of shoots of the male clone was achieved in Murashige and Skoog medium at high BA concentration (8.9 μM). For the female clone, high shoot proliferation was obtained in Crézé Beauchesne salt medium at all benzyladenine levels (2.2, 4.4, 8.9 μM) and in Murashige and Skoog with thidiazuron.
A low concentration of indolebutyric acid was effective to promote root formation, but acclimatization proved to be difficult and all plantlets died.
High proliferation of shoots of the male clone was achieved in Murashige and Skoog medium at high BA concentration (8.9 μM). For the female clone, high shoot proliferation was obtained in Crézé Beauchesne salt medium at all benzyladenine levels (2.2, 4.4, 8.9 μM) and in Murashige and Skoog with thidiazuron.
A low concentration of indolebutyric acid was effective to promote root formation, but acclimatization proved to be difficult and all plantlets died.
Authors
G. Ponchia, G. Zanin
Keywords
Skimmia japonica, 6-benzyladenine, indolebutyric acid, kinetine, micropropagation, thidiazuron
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