Articles
FACTORS AFFECTING THE GROWTH OF RANUNCULUS IN VITRO
Article number
520_17
Pages
163 – 170
Language
Abstract
In an attempt to improve the in vitro propagation of Ranunculus asiaticus L. through axillary bud multiplication, different factors were studied.
The effect of changing the amount of medium components available for each plant was evaluated by altering the plant density and by refreshing the medium.
Under our conditions, the suitable plant density was found at 10–15 shoots/vessel.
Refreshing the medium resulted in a fresh and dry weight gain higher than the control plants.
Improving air-exchange during the culture period without renewing the medium, improved shoot elongation and allowed a better leaf development.
The percentage of shoots with chlorotic leaves could be reduced by replacing the medium or by air exchanges made over the culture period.
When no subcultures or air exchanges were provided to the cultures, shoots presented a poor development and a poor capacity of control of water loss but a higher number of new plantlets was obtained at the end of the culture period.
The type of sugar in the tissue culture medium was an additional factor affecting growth of plants in vitro; Ranunculus shoots evidenced a preferential uptake of fructose.
In the different trials, the development of Ranunculus plantlets was evaluated by recording the fresh and dry weight gain and multiplication rate.
Green colour of the leaves, the plant vigour and the presence of hyperhydricity were determined by visual inspections.
The effect of changing the amount of medium components available for each plant was evaluated by altering the plant density and by refreshing the medium.
Under our conditions, the suitable plant density was found at 10–15 shoots/vessel.
Refreshing the medium resulted in a fresh and dry weight gain higher than the control plants.
Improving air-exchange during the culture period without renewing the medium, improved shoot elongation and allowed a better leaf development.
The percentage of shoots with chlorotic leaves could be reduced by replacing the medium or by air exchanges made over the culture period.
When no subcultures or air exchanges were provided to the cultures, shoots presented a poor development and a poor capacity of control of water loss but a higher number of new plantlets was obtained at the end of the culture period.
The type of sugar in the tissue culture medium was an additional factor affecting growth of plants in vitro; Ranunculus shoots evidenced a preferential uptake of fructose.
In the different trials, the development of Ranunculus plantlets was evaluated by recording the fresh and dry weight gain and multiplication rate.
Green colour of the leaves, the plant vigour and the presence of hyperhydricity were determined by visual inspections.
Authors
M. Beruto, C. Portogallo
Keywords
Ranunculus asiaticus L., in vitro, MS nutrients
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