Articles
POTENTIAL APPLICATION OF JASMONIC ACID FOR SOLANUM TUBEROSUM MICROPROPAGATION
Article number
520_13
Pages
127 – 134
Language
Abstract
In potato, Solanum tuberosum L., jasmonic acid (JA) is known as a tuber promoting substance in vitro. Since JA may also affect vegetative and root development, its potential use in enhancing potato micropropagation was investigated.
Node explants of cv.
Kennebec were grown in MS medium with or without 1 mg/l JA under different photoperiods, and micropropagated twice in Magenta boxes.
The rate of micropropagation was not significantly affected by photoperiod or JA, although it was highest under continuous light, both in controls and in JA-treated explants.
JA promoted development and growth for all photoperiods tested, and average dry weight increases of micropropagated single node explants and of plantlet root systems being over 60% and 300%, respectively.
The rise in weight of the single node sections was a consequence of an increased vegetative growth: JA-treated plantlets had larger leaf areas, thicker stems, and stronger root systems.
JA slightly decreased the dry weight percentage of the single node sections but not that of the roots.
These results suggest that JA could stimulate root formation and also play an important role in the vegetative development of the in vitro cultured potato explants.
Both effects could be used for improving the micropropagation process by producing more vigorous plantlets of higher quality.
Node explants of cv.
Kennebec were grown in MS medium with or without 1 mg/l JA under different photoperiods, and micropropagated twice in Magenta boxes.
The rate of micropropagation was not significantly affected by photoperiod or JA, although it was highest under continuous light, both in controls and in JA-treated explants.
JA promoted development and growth for all photoperiods tested, and average dry weight increases of micropropagated single node explants and of plantlet root systems being over 60% and 300%, respectively.
The rise in weight of the single node sections was a consequence of an increased vegetative growth: JA-treated plantlets had larger leaf areas, thicker stems, and stronger root systems.
JA slightly decreased the dry weight percentage of the single node sections but not that of the roots.
These results suggest that JA could stimulate root formation and also play an important role in the vegetative development of the in vitro cultured potato explants.
Both effects could be used for improving the micropropagation process by producing more vigorous plantlets of higher quality.
Authors
Ll. Martín-Closas, S. Sol, A.M. Pelacho
Keywords
Solanum tuberosum, in vitro, Jasmonic acid, micropropagation, photoperiod, potato, root development, vegetative development
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