Articles
PROGRESS IN STRUCTURING AND MAINTAINING THE GARLIC (Allium sativum) DIVERSITY FOR THE EUROPEAN GENRES PROJECT
Article number
555_28
Pages
189 – 193
Language
English
Abstract
A part of the Gatersleben garlic (Allium sativum) collection, amounting to 50 accessions has been selected for morphological characterisation and establishment of virus-free in vitro clones.
Several characters have been described which more or less closely correspond to previously found isozyme/Random amplified polymorphic DNA (RAPD) groups: the characters describing the inflorescence formation, bulb structures and pseudostem shape have been analysed.
The clones have been screened for virus infection by means of 6 different ELISA tests.
The infection rates varied from 8 mite borne filaments virus (MbFV) to 94 % garlic common latent virus (GCLV). Most accessions were infected with more than one virus, mostly latent viruses accompanying another form.
Meristem cultures were performed to eliminate the viruses.
Meristem explants reacted in 8-33 %. The regenerated plantlets have been retested by ELISA, a total of 38 % of the plantlets have been proved virus-free.
Several characters have been described which more or less closely correspond to previously found isozyme/Random amplified polymorphic DNA (RAPD) groups: the characters describing the inflorescence formation, bulb structures and pseudostem shape have been analysed.
The clones have been screened for virus infection by means of 6 different ELISA tests.
The infection rates varied from 8 mite borne filaments virus (MbFV) to 94 % garlic common latent virus (GCLV). Most accessions were infected with more than one virus, mostly latent viruses accompanying another form.
Meristem cultures were performed to eliminate the viruses.
Meristem explants reacted in 8-33 %. The regenerated plantlets have been retested by ELISA, a total of 38 % of the plantlets have been proved virus-free.
Publication
Authors
E.R.J. Keller, A. Senula
Keywords
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