Home > VIII International Symposium on Pear > INFLUENCE OF VARIOUS FACTORS ON ADVENTITIOUS SHOOT FORMATION FROM PEAR LEAF EXPLANTS

Articles

INFLUENCE OF VARIOUS FACTORS ON ADVENTITIOUS SHOOT FORMATION FROM PEAR LEAF EXPLANTS

Article number

596_77

Pages

469 – 472

Language

English

Abstract

Pear was regenerated through a callus stage and various factors were studied on shoot regeneration from leaf and petiole explants.
Placing the adaxial surface of leaf explants on regeneration medium improved shoot regeneration for Burakovka but not for Pamyat Yakovleva or GP 217. In Burakovka, precultivation of explants in liquid regeneration medium was beneficial.
The effect of wounding explants by microparticles depended on genotype.
Leaf explants taken from the non-rooted, proliferation stage produced more regeneration in Kosmicheskaya and Burakovka than from rooted shoots but shoots of Burakovka were vitrified.
Repeated cycles of culture and regeneration did not influence regeneration frequency.

Publication

VIII International Symposium on Pear

Authors

V.G. Lebedev, S.V. Dolgov

Keywords

Pyrus communis, in vitro, regeneration, orientation, pretreatment

Full text

https://doi.org/10.17660/ActaHortic.2002.596.77

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