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Articles

PLANT REGENERATION FROM CELL SUSPENSION PROTOPLASTS OF ACTINIDIA DELICIOSA

Article number

610_26

Pages

197 – 202

Language

English

Abstract

A protocol was developed for the isolation, culture and plant regeneration of protoplasts isolated from cell suspension cultures of the kiwifruit cultivar Qinxiang’ (Actinidia deliciosa). Stirring the initiated calli to fine cell aggregates and the supplementing the medium with 0.01% Pluronic F-68 were essential for cell suspension establishment and subculture.
A large number of viable protoplasts were obtained from the 4 h enzyme digestion of 2-3 d cell suspension subcultures.
Nurse culture was beneficial to A. deliciosa protoplasts culture and led to a 19.5% of plating efficiency.

Publication

V International Symposium on Kiwifruit

Authors

L. Gan, X. Xiong, R. Wang, J.B. Power, M.R. Davey

Keywords

Actinidia deliciosa, cell suspension, nurse culture, regeneration

Full text

https://doi.org/10.17660/ActaHortic.2003.610.26

Online Articles (73)

2. THE PRODUCTION AND MARKETING OF NEW ZEALAND KIWIFRUIT

R.A. Martin

3. EXPLORATION OF ACTINIDIA GENETIC RESOURCES AND DEVELOPMENT OF KIWIFRUIT INDUSTRY IN CHINA

H. Huang | S. Wang | Z. Jiang | Z. Zhang | J. Gong

4. ECONOMIC ASPECTS OF THE FRENCH KIWIFRUIT SECTOR: IMPACT OF PROFIT MARGIN POLICY ON CHANNEL OPERATION

J.-M. Fournier | D. Hassan | S. Monier-Dilhan

5. A RESEARCH APPROACH TO ENHANCE INNOVATION IN THE NEW ZEALAND KIWIFRUIT INDUSTRY

K.N. Buxton | E.W. Hewett | J.I. Reid | E.A. Cameron

6. FRUIT QUALITY ASSESSMENT OF TWELVE INTRODUCED KIWIFRUIT VARIETIES PLANTED IN CHINA

S. Zhao | M. Wang | G. Yang | L. Zhang | J. Zhou

7. KIWIFRUIT RESEARCH AND COMMERCIAL DEVELOPMENT IN HUNAN PROVINCE, CHINA

Z. Wang | C. Zhong

8. THE PLANT BREEDING CHALLENGES TO MAKING KIWIFRUIT A WORLDWIDE MAINSTREAM FRESH FRUIT

A.G. Seal

9. DEVELOPMENT AND COMMERCIALIZATION OF ‘BABY KIWI’ (ACTINIDIA ARGUTA PLANCH.)

10. INTEGRATING BOTH NUCLEAR AND CYTOPLASMIC GENETIC INFORMATION INTO A HIGH GENOMIC COVERAGE CONSERVATION APPROACH FOR ACTINIDIA

H. Huang

11. A NEW KIWIFRUIT CULTIVAR ‘SHIMEI’ FROM ACTINIDIA DELICIOSA

J. Li | X. Wang | L. Mo | R. Li | M. Liang

12. EVALUATION OF CHARACTERISTICS OF ACTINIDIA INTERSPECIFIC HYBRID ‘KOSUI’

13. EVALUATION OF THE SPORT SELECTED FROM ACTINIDIA DELICIOSA CV. HAYWARD

I. Zhu | S. Ding

14. SELECTION OF A NEW RED-FLESHED KIWIFRUIT CULTIVAR ‘HONGYANG’

M. Wang | M. Li | A. Meng

15. THE SELECTION AND CULTIVATION OF ‘QINXIANG’ ACTINIDIA

16. ‘G3’- AN EARLY MATURING SELECTION OF ACTINIDIA DELICIOSA

17. ARE KIWIFRUIT REALLY GOOD FOR YOU?

A.R. Ferguson | L.R. Ferguson

18. USE OF LIGHT REFLECTIVE MULCH TO AFFECT YIELD AND FRUIT QUALITY

G. Costa

19. EVALUATION OF VINEYARD CONDITIONS USEFUL FOR PREDICTING FRUIT QUALITY OF ‘KORYOKU’ KIWIFRUIT

K. Suezawa | H. Noda | T. Fukuda

20. LEAF AND SOIL NUTRITIONAL STATUS OF THE ‘QINMEI’ KIWIFRUIT ORCHARDS IN SHAANXI PROVINCE

L. Zhang | C. Yao | J. Liang | C. Wu | Y. Zhang

21. SHOOT SIZE AND FRUIT POSITION ALONG THE SHOOT INFLUENCES FRUIT WEIGHT OF ‘HAYWARD’ KIWIFRUIT (ACTINIDIA DELICIOSA)

M.B. Kulczewski

22. THE EFFECT OF HYDROGEN CYANAMIDE ON BUD BREAK AND YIELD OF KIWIFRUIT IN NORTHWEST PORTUGAL

A. Veloso | M. Oliveira | M.D.C. Antunes

23. UTILIZATION OF VARIOUS CHARCOAL POWDERS AS NEW ECONOMICAL POLLEN DILUENT FOR KIWIFRUIT POLLINATION

24. FLOWERING AND FRUITING MORPHOLOGY OF HARDY KIWIFRUIT, ACTINIDIA ARGUTA

C. Tiyayon | B. Strik

25. NON-DESTRUCTIVE ASSESSMENT METHODS OF KIWIFRUIT QUALITY

G. Costa | M. Noferini | M. Montefiori | S. Brigati

26. DEVELOPMENTAL CHANGES IN XYLEM FUNCTIONALITY IN KIWIFRUIT FRUIT: IMPLICATIONS FOR FRUIT CALCIUM ACCUMULATION

B. Dichio | D. Remorini | S. Lang

27. PLANT REGENERATION FROM CELL SUSPENSION PROTOPLASTS OF ACTINIDIA DELICIOSA

L. Gan | X. Xiong | R. Wang | J.B. Power | M.R. Davey

28. FACTORS AFFECTING ETHYLENE PRODUCTION OF HAYWARD KIWIFRUIT

J. Feng | K.M. Maguire | B.R. MacKay

29. VARIATION IN FIRMNESS OF PACKED HAYWARD KIWIFRUIT

J. Feng | B.R. Mackay | K.M. Maguire

30. PHYSIO-BIOCHEMICAL CHANGES AND METABOLISM OF ACTIVE OXYGEN DURING COLD STORAGE IN ACTINIDIA DELICIOSA

J. Chen | R. Zeng | P. Li

31. THE USE OF FRUIT PULPS TO EXPLORE FLAVOUR IN KIWIFRUIT

32. MODIFIED ATMOSPHERE PACKAGING OF ‘HAYWARD’ KIWIFRUIT: COMPOSITION OF THE STORAGE ATMOSPHERE AND QUALITY CHANGES AT 10 AND 20°C

S. Kitsiou | E. Sfakiotakis

33. COMMERCIAL PRACTICES USED FOR CONTROLLED ATMOSPHERE STORAGE OF ‘HAYWARD’ KIWIFRUIT

N. Lallu | J. Burdon | C.W. Yearsley | D. Billing

34. AN UNUSUAL WOOD DISEASE OF KIWIFRUIT IN ITALY

35. THE SELECTION AND CULTIVATION OF ‘QINXIANG’ ACTINIDIA

I. Petkou | T. Pritsa | E. Sfakiotakis

36. ‘HORT16A’ KIWIFRUIT: PROGRESS AND ISSUES WITH COMMERCIALISATION

K. Patterson | J. Burdon | N. Lallu

37. INFLUENCE OF GROWING TECHNIQUES ON THE INCIDENCE OF BOTRYTIS CINEREA IN COLD STORED KIWIFRUIT

S. Brigati | S. Gualanduzzi | P. Bertolini | G. Spada

38. NEW PROCEDURES FOR “CURING” AND CONTROLLED ATMOSPHERE (CA) STORAGE TO CONTROL BOTRYTIS CINEREA IN KIWIFRUIT

S. Brigati | R. Gregori | F. Neri | G.C. Pratella

39. THE WOOD DECAY OF KIWIFRUIT AND FIRST CONTROL MEASURES

S. Di Marco | F. Osti | G. Spada

40. A SYSTEMS APPROACH FOR QUALITY ASSESSMENT OF KIWIFRUIT DURING THE POSTHARVEST HANDLING, STORAGE AND SHELF LIFE

E. Sfakiotakis | M. Tzitzira | G. Stilianaki | A. Karassaridou | D. Branioti

41. PROGRESS OF RESEARCH ON POSTHARVEST PHYSIOLOGY OF KIWIFRUIT IN CHINA

T. Han | L. Li | H. Zhang

42. EFFECT OF PREHARVEST CA-CHELATE TREATMENT ON THE STORAGE QUALITY OF KIWIFRUIT

M. Xie | G.H. Jiang | H.Q. Zhang | K. Kawada

43. STUDY ON THE PORTABLE MINI COLD STORAGE ROOM AND IT¿S EFFECT ON THE QUALITY OF KIWIFRUIT

X. Li | W. Guan | L. Chen | Y. Hu

44. CALCIUM ABSORPTION AND DISTRIBUTION IN MATURE KIWIFRUIT PLANTS

C. Xiloyannis | G. Celano | G. Montanaro | B Dichio

45. SCREENING CHLOROPLAST, MITOCHONDRIAL, AND NUCLEAR DNA SEQUENCES SUITABLE FOR TAXONOMIC STUDIES IN ACTINIDIACEAE

46. MOLECULAR CHARACTERIZATION OF KIWIFRUIT VARIETIES USING SSR MARKERS

Y. Zheng | Z. Li | H. Huang

47. PHYLOGENETIC CLASSIFICATION OF INTRODUCED AND INDIGENOUS ACTINIDIA IN JAPAN AND IDENTIFICATION OF INTERSPECIFIC HYBRIDS USING RAPD ANALYSIS

48. SCREENING MICROSATELLITES FOR THEIR EFFECTIVENESS TO IDENTIFY AND DIFFERENTIATE AMONG ACTINIDIA GENOTYPES

A.E. Korkovelos | C.K. Goulas | M.D. Vasilakakis

49. CONSERVATION AND UTILIZATION OF GERMPLASM RESOURCES OF THE GENUS ACTINIDIA

S. Wang | Z. Jiang | H. Huang | Z. Zhang | J. Ke

50. PROTECTION OF ENDANGERED ACTINIDIA GERMPLASM RESOURCES IN THE SHENNONGJIA MOUNTAINS

51. CYTOGENETIC STUDY OF DIPLOID ACTINIDIA CHINENSIS – KARYOTYPE, MORPHOLOGY OF SEX CHROMOSOMES AT PRIMARY DIFFERENTIATION STAGE AND EVOLUTIONARY SIGNIFICANCE

Z. He | H. Huang | Y. Zhong

52. PHYLOGENETIC RELATIONSHIPS IN ACTINIDIA AS REVEALED BY RAPDS AND PCR-RFLPS OF MTDNA

Z. Li | H. Huang | Z. Jiang | J. Li | T.L. Kubisiak

53. EFFECTIVENESS OF COPPER COMPOUNDS IN THE CONTROL OF BACTERIAL DISEASES OF KIWIFRUIT PLANTS

G.M. Balestra | M. Bovo

54. CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV. ACTINIDIAE ISOLATED IN KOREA AND GENETIC RELATIONSHIP AMONG CORONATINE-PRODUCING PATHOVARS BASED ON CMAU SEQUENCES

H. Shim Han | E. Jung Oak | Y. Jin Koh | J.-S. Hur | J. Sung Jung

55. FIRST OBSERVATIONS OF A WOOD DECAY (ESCA-LIKE DISEASE) ON KIWIFRUIT IN FRANCE

56. MODELLING OF SCLEROTINIA DISEASE IN NEW ZEALAND KIWIFRUIT (ACTINIDIA DELICIOSA)

S.M. Hoyte | P. Long | R.M. Beresford

57. ANTIFUNGAL PROPERTIES OF EUCALYPTUS DARLYMPLEANA AGAINST POSTHARVEST PATHOGENS OF KIWIFRUITS

58. EFFECTS OF OZONATED WATER ON POSTHARVEST PATHOGENS OF KIWIFRUITS IN LABORATORY

J.S. Hur | J.A. Kim | J.S. Jung | Y.J. Koh

59. CURRENT STATUS OF OCCURRENCE OF MAJOR DISEASES ON KIWIFRUITS AND THEIR CONTROL IN KOREA

Y.J. Koh | J.S. Jung | J.S. Hur

60. INFLUENCE OF GRAPE VOLATILES OF VITIS LABRUSCA ‘ISABELL’ ON GROWTH OF BOTRYTIS CINEREA ON ‘HAYWARD’ KIWIFRUIT

E.K. Kulakiotu | E.M Sfakiotakis

61. RESEARCH ON KIWIFRUIT SOURCE OF GENETIC RESISTANCE TO ROOT-KNOT AND LESION NEMATODES

A. Nicotra | A.M. Simeone | M. De Vito

62. MULTIPLE GENE COPIES: CARRYING OUT BIOCHEMICAL AND MOLECULAR STUDIES IN KIWIFRUIT

E. MacRae | R. Atkinson

63. WHOLE-PLANT GAS EXCHANGES AND CARBOHYDRATE METABOLISM IN KIWIFRUIT

G. Vizzotto | G. Costa | M. Noferini | L. Corelli-Grappadelli

64. LIPID AND POLYSACCHARIDE VARIATIONS IN ACTINIDIA DELICIOSA DURING POLLEN ONTOGENY AND GERMINATION

I. Abreu | M. Oliveira

65. CFD SIMULATION OF FLOW AROUND A SINGLE KIWIFRUIT FLOWER

M.J.W. Hii | J. Abrahamson | P.J. Jordan

66. PRESENCE OF PROTEINS, CALLOSE AND PECTINS IN UNGERMINATED AND GERMINATED POLLEN OF ACTINIDIA DELICIOSA

M. Oliveira | I. Abreu

67. GENE TRANSFER STRATEGY FOR KIWIFRUIT TO OBTAIN PURE TRANSGENIC PLANT THROUGH INDUCING ADVENTITIOUS ROOTS IN LEAF EXPLANTS WITH AGROBACTERIUM TUMEFACIENS

M. Li | Z. Huang | L. Han | G. Zhao | Y. Li

68. A HIGH EFFICIENT AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION SYSTEM FOR KIWIFRUIT

M. Li | Z. Huang | L. Han | J. Yao

69. EFFECTS OF SHADE LEVEL ON KIWIFRUIT LEAF EFFICIENCY IN A MARGINAL AREA

P. Allan | C. Carlson

70. LINKAGE MAP IN KIWIFRUIT: THE CHOICE OF MAPPING POPULATIONS

71. APPLICATION OF MODERN BIOTECHNOLOGY ON KIWIFRUIT

X. Xu | X. Yao | H. Chen

72. MOLECULAR MARKERS FOR EARLY SEX DETERMINATION IN ACTINIDIA

X. Xiao | L. Zhang | S. Li | R. Testolin | G. Cipriani

73. COMPARISON OF CPPU EFFECT ON FRUIT DEVELOPMENT IN SEVERAL ACTINIDIA SPECIES