Articles

LIPID AND POLYSACCHARIDE VARIATIONS IN ACTINIDIA DELICIOSA DURING POLLEN ONTOGENY AND GERMINATION

Article number
610_63
Pages
473 – 477
Language
English
Abstract
Sudan black B, iodine potassium iodide solution (IKI test) and periodic acid-Schiff (PAS) reagent were used for the histochemical detection of lipids, starch and polysaccharides in resin-embedded semi-thin sections and the results were confirmed at the ultra-structural level, using transmission electron microscopy. During germination, lipids and starch were hydrolysed and probably used as energy sources for the rapidly growing pollen tubes.
The insoluble polysaccharides presented a similar distribution to the one observed with IKI but they were not completely consumed persisting in the cytoplasm.

Publication
Authors
I. Abreu, M. Oliveira
Keywords
kiwifruit, pollen grain, pollen tube, sudan black B, IKI test, PAS reagent.
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