Articles
IN VITRO ADVENTITIOUS REGENERATION AFFECTS THE GENETIC STABILITY OF STRAWBERRY PLANTS (FRAGARIA X ANANASSA DUCH.)
Article number
616_71
Pages
459 – 462
Language
English
Abstract
At the insertion site of the petiole with the stipule regenerated plants of the strawberry cultivars Teodora, Clea and Egla were obtained.
All the cultivars showed similar regeneration percentages.
The genetic variation induced by tissue culture technique was detected with the RAPD molecular markers.
DNA fingerprinting was carried out with several ten-mer random primers.
Samples of plants were randomly chosen among the total of regenerated plants of each cultivar and compared to those of the mother plants.
A cluster analysis, based on the totality of scorable RAPD bands, was performed to construct a genetic similarity dendrogram.
The results showed genetic variability between mother plants and regenerated plants.
All the cultivars showed similar regeneration percentages.
The genetic variation induced by tissue culture technique was detected with the RAPD molecular markers.
DNA fingerprinting was carried out with several ten-mer random primers.
Samples of plants were randomly chosen among the total of regenerated plants of each cultivar and compared to those of the mother plants.
A cluster analysis, based on the totality of scorable RAPD bands, was performed to construct a genetic similarity dendrogram.
The results showed genetic variability between mother plants and regenerated plants.
Publication
Authors
M.A. Palombi, S. Monticelli, A. Festa, C. Damiano
Keywords
Genetic variability, in vitro techniques, RAPD (Random Amplified Polymorphic DNA)
Online Articles (80)
