Articles
TWO CRINIVIRUSES ARE ASSOCIATED WITH THE STRAWBERRY PALLIDOSIS DISEASE
Article number
656_1
Pages
21 – 26
Language
English
Abstract
Pallidosis, a disease attributed to a graft-transmissible agent is the focal point of this study.
Two viruses belonging to the Closteroviridae family, genus Crinivirus that can cause pallidosis symptoms on strawberry indicator plants have been identified.
A previously unidentified virus designated as Strawberry pallidosis associated virus (SPaV) is the predominant virus in pallidosis positive plants, while the second virus is Beet pseudo-yellows virus (BPYV). The genomes of both viruses have been sequenced fully and phylogenetic analyses indicate that the two viruses are more closely related than any other crinivirus found in the database.
Epidemiological studies have demonstrated that the greenhouse whitefly, Trialeuroides vaporariorum, is an efficient vector of SPaV. Protocols for molecular detection of both viruses using reverse transcription-polymerase chain reaction (RT-PCR) have been developed.
The recombinant major coat protein of SPaV has been expressed in bacteria and polyclonal antibodies to the virus have been developed that facilitate detection of the virus in tissue blot immunoassays (TBIA). The potential of seed and pollen transmission of SPaV was examined as an alternative mode of transmission of the virus.
The geographical distribution of both viruses in the major strawberry producing regions of the United States has been examined.
Two viruses belonging to the Closteroviridae family, genus Crinivirus that can cause pallidosis symptoms on strawberry indicator plants have been identified.
A previously unidentified virus designated as Strawberry pallidosis associated virus (SPaV) is the predominant virus in pallidosis positive plants, while the second virus is Beet pseudo-yellows virus (BPYV). The genomes of both viruses have been sequenced fully and phylogenetic analyses indicate that the two viruses are more closely related than any other crinivirus found in the database.
Epidemiological studies have demonstrated that the greenhouse whitefly, Trialeuroides vaporariorum, is an efficient vector of SPaV. Protocols for molecular detection of both viruses using reverse transcription-polymerase chain reaction (RT-PCR) have been developed.
The recombinant major coat protein of SPaV has been expressed in bacteria and polyclonal antibodies to the virus have been developed that facilitate detection of the virus in tissue blot immunoassays (TBIA). The potential of seed and pollen transmission of SPaV was examined as an alternative mode of transmission of the virus.
The geographical distribution of both viruses in the major strawberry producing regions of the United States has been examined.
Authors
I.E. Tzanetakis, A.B. Halgren, W.M. Wintermantel, K.E. Keller, R.R. Martin
Keywords
Fragaria x ananassa, pallidosis, Strawberry pallidosis associated virus, Beet pseudo-yellows virus, Trialeuroides vaporariorum, detection
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