Articles
CHARACTERIZATION OF BLACKCURRANT REVERSION ASSOCIATED VIRUS ISOLATES COLLECTED FROM RIBES PLANTATIONS IN HUNGARY
Article number
656_19
Pages
125 – 129
Language
English
Abstract
The reversion disease of black currant has been observed in Hungary since the middle of 60s.
The presence of the causal agent of this disease, the Blackcurrant reversion associated virus (BRAV) was confirmed by indexing and by RT-PCR in 2000-2001. Visiting old currant orchards in spring 2003 reverted red currant plants were also found.
This was the first time when severe flowers symptoms on black currant were observed in our country and in the case of red currant the flower symptoms also were characteristic.
Black- and red currant samples of symptomatic plants were collected and tested by mechanical inoculation on Chenopodium quinoa and by RT-PCR using five different primer sets.
One of the sets was specific for the viral coat protein gene, the other four sets amplified segments from the RNA2 3-NTR. The presence of big buds on reverted plants was noted and the gall mite vector of BRAV: Cecidophyopsis spp. was checked in the big buds by light microscopy.
This is the first report of BRAV infecting red currant in Hungary.
The presence of the causal agent of this disease, the Blackcurrant reversion associated virus (BRAV) was confirmed by indexing and by RT-PCR in 2000-2001. Visiting old currant orchards in spring 2003 reverted red currant plants were also found.
This was the first time when severe flowers symptoms on black currant were observed in our country and in the case of red currant the flower symptoms also were characteristic.
Black- and red currant samples of symptomatic plants were collected and tested by mechanical inoculation on Chenopodium quinoa and by RT-PCR using five different primer sets.
One of the sets was specific for the viral coat protein gene, the other four sets amplified segments from the RNA2 3-NTR. The presence of big buds on reverted plants was noted and the gall mite vector of BRAV: Cecidophyopsis spp. was checked in the big buds by light microscopy.
This is the first report of BRAV infecting red currant in Hungary.
Authors
K. Nyerges, I. Ember, L. Krizbai
Keywords
BRAV, black currant, red currant, RT-PCR, “big bud”, Cecidophyopsis spp., flower symptoms
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