Articles
DEVELOPMENT OF RESISTANCE TO RASPBERRY BUSHY DWARF VIRUS IN ‘MEEKER’ RED RASPBERRY
Article number
656_26
Pages
165 – 169
Language
English
Abstract
Raspberry bushy dwarf virus (RBDV) has been a reemerging problem over the past 15 years throughout raspberry growing regions of the world.
This is due primarily to growers planting susceptible cultivars that have superior fruit quality.
Crumbly fruit and yield reduction can combine to reduce crop value by more than 50%. RBDV is pollen-borne, making chemical control virtually impossible. Meeker raspberry plants were transformed with the coat protein gene, mutated forms of the movement protein gene or nontranslatable RNA of RBDV. The presence of these viral sequences was confirmed by DNA hybridization analysis and RT-PCR of DNAse digested total RNA preparations.
Of 197 transgenic lines planted in replicated blocks in the field under extreme disease pressure, five lines remained free of RBDV after four years.
All 202 of the wild-type Meeker plants in the same plot were infected with RBDV at the end of four years.
Most transgenic lines had 9 of 9 plants infected and a few lines showed partial resistance to infection, with 1 to 6 of the 9 plants infected.
The five lines showing field resistance were also resistant to RBDV when tested by grafting.
Fruit evaluations of these five lines showed that they contained the five anthocyanins at the same relative concentrations as were present in wild type Meeker fruit.
Field and fruit evaluations of these five lines will be continued for another two years.
This is due primarily to growers planting susceptible cultivars that have superior fruit quality.
Crumbly fruit and yield reduction can combine to reduce crop value by more than 50%. RBDV is pollen-borne, making chemical control virtually impossible. Meeker raspberry plants were transformed with the coat protein gene, mutated forms of the movement protein gene or nontranslatable RNA of RBDV. The presence of these viral sequences was confirmed by DNA hybridization analysis and RT-PCR of DNAse digested total RNA preparations.
Of 197 transgenic lines planted in replicated blocks in the field under extreme disease pressure, five lines remained free of RBDV after four years.
All 202 of the wild-type Meeker plants in the same plot were infected with RBDV at the end of four years.
Most transgenic lines had 9 of 9 plants infected and a few lines showed partial resistance to infection, with 1 to 6 of the 9 plants infected.
The five lines showing field resistance were also resistant to RBDV when tested by grafting.
Fruit evaluations of these five lines showed that they contained the five anthocyanins at the same relative concentrations as were present in wild type Meeker fruit.
Field and fruit evaluations of these five lines will be continued for another two years.
Authors
R.R. Martin, K.E. Keller, H. Mathews
Keywords
Raspberry bushy dwarf virus, coat protein, resistance, fruit quality
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