Articles
OCCURRENCE AND IDENTIFICATION OF STRAWBERRY VIRUSES IN FIVE EUROPEAN COUNTRIES
Article number
656_4
Pages
39 – 43
Language
English
Abstract
More than 30 viruses and phytoplasmas affecting the genus Fragaria have been described.
The most important are the aphid-borne viruses: Strawberry crinkle virus (SCV), Strawberry mottle virus (SMoV), Strawberry mild yellow edge virus (SMYEV) and Strawberry vein banding virus (SVBV). These viruses do not induce distinct symptoms in commercial cultivars but often cause a loss of vigour, stunting, and decreased yield in infected plants.
The presence and identification of the main strawberry aphid-borne viruses in five European countries (Italy, Poland, The Czech Republic, Germany and Lithuania) was investigated.
Leaf samples of plants of different cultivars were collected and tested for the presence of SCV, SMoV, SMYEV and SVBV by grafting onto biological indicators (leaflet grafting on F. vesca and F. virginiana). Arabis mosaic virus, Raspberry ring spot virus, Strawberry latent ring spot virus, Tomato black ring virus, Tomato ring spot virus, Tobacco necrosis virus, Tobacco streak virus as well as SMYEV tested for by ELISA. Sensitivity of the biological tests was compared with the recently developed molecular methods: NASBA-based detection technique (AmpliDetRNA) and RT-PCR using primer pairs specific for SMoV, SCV, SVBV, and SMYEV. In total, approximately 4% of all tested strawberry plants were positive for at least one virus.
Of the aphid-borne viruses, SMoV, SCV and SMYEV were most common, while SVBV was not detected.
The nucleic acid based detection techniques were as sensitive as biological indexing and in some cases virus detection based on AmpliDetRNA and RT-PCR was more sensitive than the biological tests.
The most important are the aphid-borne viruses: Strawberry crinkle virus (SCV), Strawberry mottle virus (SMoV), Strawberry mild yellow edge virus (SMYEV) and Strawberry vein banding virus (SVBV). These viruses do not induce distinct symptoms in commercial cultivars but often cause a loss of vigour, stunting, and decreased yield in infected plants.
The presence and identification of the main strawberry aphid-borne viruses in five European countries (Italy, Poland, The Czech Republic, Germany and Lithuania) was investigated.
Leaf samples of plants of different cultivars were collected and tested for the presence of SCV, SMoV, SMYEV and SVBV by grafting onto biological indicators (leaflet grafting on F. vesca and F. virginiana). Arabis mosaic virus, Raspberry ring spot virus, Strawberry latent ring spot virus, Tomato black ring virus, Tomato ring spot virus, Tobacco necrosis virus, Tobacco streak virus as well as SMYEV tested for by ELISA. Sensitivity of the biological tests was compared with the recently developed molecular methods: NASBA-based detection technique (AmpliDetRNA) and RT-PCR using primer pairs specific for SMoV, SCV, SVBV, and SMYEV. In total, approximately 4% of all tested strawberry plants were positive for at least one virus.
Of the aphid-borne viruses, SMoV, SCV and SMYEV were most common, while SVBV was not detected.
The nucleic acid based detection techniques were as sensitive as biological indexing and in some cases virus detection based on AmpliDetRNA and RT-PCR was more sensitive than the biological tests.
Authors
A.R. Babini, M. Cieślińska, R. Kareová, J.R. Thompson, M. Cardoni
Keywords
SMoV, SCV, SMYEV, SVBV, biological indexing, RT-PCR, AmpliDet RNA
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