Articles
LOCALIZATION OF DETERMINANTS FOR ANTIGENICITY AND MITE-TRANSMISSION USING STRUCTURAL MODEL OF BLACKCURRANT REVERSION VIRUS
Article number
656_15
Pages
103 – 108
Language
English
Abstract
Structural model for Blackcurrant reversion virus (BRV; genus Nepovirus) was created using Tobacco ringspot virus (TRSV) as a template.
The primary coordinates for the structure of the coat protein (CP) and its constructed protein model were obtained using the programs at Expert Protein Analysis System (ExPASy) proteomics server of the Swiss Institute of Bioinformatics (SIB) (http://us.expasy.org/) and in Protein Data Bank (www.rcsb.org/pdb/), and the virion model was constructed using molecular modeling tools.
The structures of BRV and TRSV were highly similar except for few specific motifs and the extended C-terminus of BRV-CP, which was located on the surface of virions.
Instead, the N-terminus of BRV coat protein was located towards the interior of virions.
Several potential antigenic determinants along the CP sequence were identified that were localized on the surface of coat protein and virions.
These include C-terminal segments that have been used previously to generate peptide antibodies.
The two putative mite-transmission determinants, STS and KAG, were also located on the surface of virions.
The data obtained using structural modelling may be superior for defining useful sites for antibody generation compared to conventional protein analysis programs, and is being used to develop functional phage antibodies to BRAV.
The primary coordinates for the structure of the coat protein (CP) and its constructed protein model were obtained using the programs at Expert Protein Analysis System (ExPASy) proteomics server of the Swiss Institute of Bioinformatics (SIB) (http://us.expasy.org/) and in Protein Data Bank (www.rcsb.org/pdb/), and the virion model was constructed using molecular modeling tools.
The structures of BRV and TRSV were highly similar except for few specific motifs and the extended C-terminus of BRV-CP, which was located on the surface of virions.
Instead, the N-terminus of BRV coat protein was located towards the interior of virions.
Several potential antigenic determinants along the CP sequence were identified that were localized on the surface of coat protein and virions.
These include C-terminal segments that have been used previously to generate peptide antibodies.
The two putative mite-transmission determinants, STS and KAG, were also located on the surface of virions.
The data obtained using structural modelling may be superior for defining useful sites for antibody generation compared to conventional protein analysis programs, and is being used to develop functional phage antibodies to BRAV.
Authors
M. Hohkuri, U. Lamminmäki, T. Wahlroos, P. Susi
Keywords
BRV, coat protein, molecular modelling, Ribes nigrum L., virions
Online Articles (31)