Articles
CHARACTERIZATION AND CONTROL OF PINEAPPLE MEALYBUG WILT ASSOCIATED AMPELOVIRUSES
Article number
702_1
Pages
23 – 27
Language
English
Abstract
Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. Pineapple mealybug wilt associated virus-2 (PMWaV-2) infection and mealybug exposure are involved in the etiology of MWP whereas PMWaV-1 is not a necessary component in MWP but correlates with reduced yields.
Degenerate primers derived from conserved sequence regions of the HSP-70 homolog gene of various Ampelo¬viruses were used in RT-PCR assays to identify additional viruses in pineapple.
Our results show that PMWaV-3 shares 64%, 73%, and 79% amino acid identity with PMWaV-1 in the RNA dependent RNA polymerase, small hydrophobic protein, and HSP-70 homolog ORFs, respectively.
Shared amino acid identity with PMWaV-2 in the same ORFs is 30%, 13%, and 44%. Preliminary data have shown that PMWaV-3 is more closely related to PMWaV-1 than to PMWaV-2. The coat protein gene of PMWaV-2 was constructed as an inverted-repeat in pCAMBIA 1300 vector and used to produce transgenic pineapple plants using Agrobacterium-mediated transformation.
At least five lines of putatively transgenic pineapple plants that are resistant to PMWaV-2 infection have been produced.
These plants have remained resistant to PMWaV-2 infection after multiple challenges with viruliferous mealybugs and have not developed any MWP symptoms after several months.
Degenerate primers derived from conserved sequence regions of the HSP-70 homolog gene of various Ampelo¬viruses were used in RT-PCR assays to identify additional viruses in pineapple.
Our results show that PMWaV-3 shares 64%, 73%, and 79% amino acid identity with PMWaV-1 in the RNA dependent RNA polymerase, small hydrophobic protein, and HSP-70 homolog ORFs, respectively.
Shared amino acid identity with PMWaV-2 in the same ORFs is 30%, 13%, and 44%. Preliminary data have shown that PMWaV-3 is more closely related to PMWaV-1 than to PMWaV-2. The coat protein gene of PMWaV-2 was constructed as an inverted-repeat in pCAMBIA 1300 vector and used to produce transgenic pineapple plants using Agrobacterium-mediated transformation.
At least five lines of putatively transgenic pineapple plants that are resistant to PMWaV-2 infection have been produced.
These plants have remained resistant to PMWaV-2 infection after multiple challenges with viruliferous mealybugs and have not developed any MWP symptoms after several months.
Publication
Authors
E.P. Perez, D.M. Sether, M.J. Melzer, J.L. Busto, C. Nagai, J.S. Hu
Keywords
virus diversity, transgenic pineapple plants
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