Articles
GENETIC MAPPING OF DNA MARKERS IN PINEAPPLE
Article number
702_9
Pages
79 – 86
Language
English
Abstract
Two genetic maps of DNA-markers (RAPD, AFLP and ISSR) of Ananas comosus var. bracteatus and A. comosus var. comosus have been previously published by our team.
These maps were constructed at the Laboratory of Genetics and Plant Breeding of FERN, University of Algarve, using an F1 mapping population derived from a cross performed in Martinique between the two botanical varieties.
However, the use of a F1 population, the small size of this population and the use of molecular markers with dominant inheritance allowed only markers that were heterozygous in the parents to be included in those maps and prevented the construction of an integrated high-density map.
A new genetic map, intended to cover all the pineapple genome, is currently under construction using a mapping population of 142 F2 plants.
This new map will integrate the already published maps and will include markers that had remained unlinked, as they were polymorphic but homozygous in parental genotypes, as well as newly identified markers.
At the present time, the new map consists of seven linkage groups (> 40 cM) that integrate markers from both parents, 21 groups (> 40 cM) with markers mostly from one parent, six groups (< 40 cM and > 25 cM) with markers from one parent, and 12 smaller (<25 cM) linkage groups, covering approximately 62% of the pineapple genome.
These maps were constructed at the Laboratory of Genetics and Plant Breeding of FERN, University of Algarve, using an F1 mapping population derived from a cross performed in Martinique between the two botanical varieties.
However, the use of a F1 population, the small size of this population and the use of molecular markers with dominant inheritance allowed only markers that were heterozygous in the parents to be included in those maps and prevented the construction of an integrated high-density map.
A new genetic map, intended to cover all the pineapple genome, is currently under construction using a mapping population of 142 F2 plants.
This new map will integrate the already published maps and will include markers that had remained unlinked, as they were polymorphic but homozygous in parental genotypes, as well as newly identified markers.
At the present time, the new map consists of seven linkage groups (> 40 cM) that integrate markers from both parents, 21 groups (> 40 cM) with markers mostly from one parent, six groups (< 40 cM and > 25 cM) with markers from one parent, and 12 smaller (<25 cM) linkage groups, covering approximately 62% of the pineapple genome.
Publication
Authors
J.D. Carlier, D. Nancheva, J.M. Leitão, G. Coppens d’Eeckenbrugge
Keywords
Ananas, linkage mapping, genetic map, molecular markers, AFLP, ISSR, RAPD
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