Articles
EVIDENCE FOR THE INDUCTION OF SYSTEMIC ACQUIRED RESISTANCE (SAR) BY ACIBENZOLAR IN CULTIVATED PINEAPPLE
Article number
702_18
Pages
151 – 156
Language
English
Abstract
Acibenzolar induces Systemic Acquired Resistance (SAR) in many plants and its application to Ananas comosus reduced reproduction of Rotylenchulus reniformis and Meloidogyne.
To determine whether SAR was induced by the acibenzolar treatment, the expression of the pathogenesis-related protein [PR-1] gene transcript was measured as a molecular marker of the SAR pathway in pineapple, Ananas comosus. One-month-old A. comosus were sprayed with 0 or 100 mg acibenzolar-s-methyl/L. Two days later, plants were removed from pots and the roots gently washed free of soil.
The roots were cut from the base of the crown, placed on ice, and RNA was immediately extracted.
First-strand cDNA was synthesized from total RNA by RT-PCR using oligo-dT primers, followed by a regular PCR using primers designed to two conserved regions of the rice basic PR-1 gene.
A PCR product of 266 bp was only detected in the RNA sample from plants treated with acibenzolar-s-methyl.
The nucleotide sequence of the PCR fragment had high identity (79 to 94%) with the PR-1 genes from other monocots and the deduced amino acid sequence was 58% to 73% similar to other PR-1 proteins.
We concluded that acibenzolar induced SAR in A. comosus and propose that SAR provided some resistance to nematode infection.
To determine whether SAR was induced by the acibenzolar treatment, the expression of the pathogenesis-related protein [PR-1] gene transcript was measured as a molecular marker of the SAR pathway in pineapple, Ananas comosus. One-month-old A. comosus were sprayed with 0 or 100 mg acibenzolar-s-methyl/L. Two days later, plants were removed from pots and the roots gently washed free of soil.
The roots were cut from the base of the crown, placed on ice, and RNA was immediately extracted.
First-strand cDNA was synthesized from total RNA by RT-PCR using oligo-dT primers, followed by a regular PCR using primers designed to two conserved regions of the rice basic PR-1 gene.
A PCR product of 266 bp was only detected in the RNA sample from plants treated with acibenzolar-s-methyl.
The nucleotide sequence of the PCR fragment had high identity (79 to 94%) with the PR-1 genes from other monocots and the deduced amino acid sequence was 58% to 73% similar to other PR-1 proteins.
We concluded that acibenzolar induced SAR in A. comosus and propose that SAR provided some resistance to nematode infection.
Publication
Authors
B. Chinnasri, D.A. Christopher, B.S. Sipes
Keywords
Ananas comosus, nematode, pathogenesis-related protein, PR-1 gene, systemic acquired resistance
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