Articles
EVALUATION OF TRANSGENE STABILITY, GENE EXPRESSION AND HERBICIDE TOLERANCE OF GENETICALLY MODIFIED PINEAPPLE UNDER FIELD CONDITIONS
Article number
702_3
Pages
37 – 40
Language
English
Abstract
Pineapple plants of the cv.
Phuket transformed with the bar gene for herbicide tolerance were micropropagated, rooted and established in a shade house before transfer to an experimental field plot.
Seven months after transfer to the field, plants were tolerant to 1600 ml rai-1 (240 g rai-1) of the herbicide Basta X®, this being twice the dose recommended for field application of the herbicide.
In contrast, non-trans¬formed pineapple plants became necrotic and died within 21 days of spraying with the herbicide at 800 ml rai-1. Bar gene stability and expression in clonally-derived plants were assessed by PCR, RT-PCR and Southern analyses at 120, 210 and 380 days following transfer of the plants to the field.
The bar gene was stable and expressed in transgenic plants throughout the duration of the trial.
Importantly, fruit quality and yield were unaffected by transformation.
Phuket transformed with the bar gene for herbicide tolerance were micropropagated, rooted and established in a shade house before transfer to an experimental field plot.
Seven months after transfer to the field, plants were tolerant to 1600 ml rai-1 (240 g rai-1) of the herbicide Basta X®, this being twice the dose recommended for field application of the herbicide.
In contrast, non-trans¬formed pineapple plants became necrotic and died within 21 days of spraying with the herbicide at 800 ml rai-1. Bar gene stability and expression in clonally-derived plants were assessed by PCR, RT-PCR and Southern analyses at 120, 210 and 380 days following transfer of the plants to the field.
The bar gene was stable and expressed in transgenic plants throughout the duration of the trial.
Importantly, fruit quality and yield were unaffected by transformation.
Publication
Authors
S. Sripaoraya, S. Keawsompong, P. Insupa, M.R. Davey, J.B. Power, P. Srinives
Keywords
Ananas comosus L. (pineapple), bar gene, field evaluation, glufosinate ammonium, fruit quality and yield
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