Articles
SLOW-GROWTH CONDITIONS FOR THE IN VITRO CONSERVATION OF PINEAPPLE GERMPLASM
Article number
702_4
Pages
41 – 45
Language
English
Abstract
Embrapa Cassava and Tropical Fruits has the largest collection of pineapple germplasm in Latin America.
However, this collection is kept in the field, subjected to losses caused by biotic and abiotic factors.
Thus, in order to minimize this problem and to assure a replicate of that collection, an in vitro one is being established, based on the cultivation of axillary buds aiming at the production of plants that can be kept in conditions of minimum growth.
Until now, 120 accessions from the material kept in the field have been replicated under in vitro conditions.
The goal is that, by the end of 2005, at least 50% of the accessions will have been replicated.
Currently, the plants are being conserved in a basic MS/2 medium, without growth regulators and at growth delaying environmental conditions (temperatures of 21 ± 2°C, photoperiod of 12 hours). In addition, other trials are being carried out seeking to further extend the time of conservation, taking into account the different behaviors observed in different varieties, in micropropagation work.
Growth regulators, such as paclobutrazol and abscisic acid, associated to different concentrations of MS salts have been tested.
Preliminary results have shown delaying effect of those regulators on the in vitro growth of pineapple genotypes.
However, this collection is kept in the field, subjected to losses caused by biotic and abiotic factors.
Thus, in order to minimize this problem and to assure a replicate of that collection, an in vitro one is being established, based on the cultivation of axillary buds aiming at the production of plants that can be kept in conditions of minimum growth.
Until now, 120 accessions from the material kept in the field have been replicated under in vitro conditions.
The goal is that, by the end of 2005, at least 50% of the accessions will have been replicated.
Currently, the plants are being conserved in a basic MS/2 medium, without growth regulators and at growth delaying environmental conditions (temperatures of 21 ± 2°C, photoperiod of 12 hours). In addition, other trials are being carried out seeking to further extend the time of conservation, taking into account the different behaviors observed in different varieties, in micropropagation work.
Growth regulators, such as paclobutrazol and abscisic acid, associated to different concentrations of MS salts have been tested.
Preliminary results have shown delaying effect of those regulators on the in vitro growth of pineapple genotypes.
Publication
Authors
F.V.D. Souza, T.L. Soares, J.R.S. Cabral, D.H. Reinhardt, J.L. Cardoso, D.A. Benjamin
Keywords
Ananas comosus var. comosus, varieties, growth regulators, MS medium
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