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Articles

MICROARRAY EXPRESSION PROFILING OF NAGAMI KUMQUAT IN RESPONSE TO CANKER

Article number

738_21

Pages

221 – 227

Language

English

Abstract

Citrus canker disease, caused by a bacterial pathogen Xanthomonas axonopodis pv. citri, affects citrus species and citrus relatives in many areas of the world.
Kumquats have apparent resistance to canker, the mechanism of which has not been determined.
Forward and reverse subtractive cDNA libraries were constructed using Nagami kumquat mRNA to identify genes that are differentially expressed during the interaction with the canker pathogen.
Some cDNA clones were selected for sequencing, and the sequences were analyzed using NCBI BLAST. Homologues to transcription factors, receptor and resistance genes known to be involved in plant-pathogen interaction were identified. cDNA microarrays containing 2304 of these genes were analyzed for expression profiles during the interaction with the canker pathogen.
The results demonstrate how the use of microarray-based expression profiling methods can help elucidate plant pathogenesis-related response mechanisms and assign roles for previously uncharacterized genes.
This can be considered to be a case study in citrus that has used these high throughput technologies to understand the defense mechanisms in Fortunella and citrus at the molecular level.

Publication

International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species

Authors

A.A. Khalaf, G.A. Moore, F.G. Gmitter Jr.

Keywords

Full text

https://doi.org/10.17660/ActaHortic.2007.738.21

Online Articles (105)

1. INTEGRATING GENOMICS INTO ROSACEAE FRUIT BREEDING

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2. GENETIC MODIFICATIONS IN FLORAL CROPS: RESEARCH TO MARKETPLACE

S. Chandler

3. RECOVERY OF DIFFICULT-TO-REGENERATE SPECIES: THE CYCAD EXAMPLE

V.M. Chávez | S.L. Cabrera | R.E. Litz | P.A. Moon

4. GENOMICS APPROACHES TO UNDERSTANDING RIPENING CONTROL AND FRUIT QUALITY IN TOMATO

J. Giovannoni

5. APPLICATIONS OF SOMATIC HYBRIDIZATION AND CYBRIDIZATION IN SCION AND ROOTSTOCK IMPROVEMENT, WITH FOCUS ON CITRUS

6. OVERCOMING CHALLENGES TO DELIVER TRANSGENIC HORTICULTURAL PRODUCTS TO U.S. AND OVERSEAS MARKETS

K. Kahn | P. Schmeissner | E. Jones | B. Richey | B. Simmons

7. CAN KNOWLEDGE ABOUT THE MOLECULAR BASES OF PLANT DISEASE AND DISEASE RESISTANCE HELP ENGINEERING RESISTANCE IN CROPS?

O. Le Gall

8. COMMERCIALIZATION OF GENETICALLY ENGINEERED PAPAYA: BREEDING PERSPECTIVES

R.M. Manshardt

9. MOLECULAR GENETICS OF WALNUTS – A BREEDER’S PERSPECTIVE

G. McGranahan

10. SITE-SPECIFIC RECOMBINATION FOR PLANT GENETIC ENGINEERING: STRATEGY FOR AGRO-MEDIATED GENE STACKING

D.W. Ow

11. CURRENT CHALLENGES OF TROPICAL TREE CROP IMPROVEMENT: INTEGRATING GENOMICS INTO AN APPLIED CACAO BREEDING PROGRAM

12. USING BIOTECHNOLOGY TO IMPROVE RESISTANCE TO ENVIRONMENTAL STRESS IN FRUIT CROPS: THE IMPORTANCE OF UNDERSTANDING PHYSIOLOGY

M. Wisniewski | C. Bassett | J.L. Norelli | T. Artlip | J. Renaut

13. IS IT POSSIBLE TO INDUCE COLD TOLERANCE IN PAPAYA THROUGH GENETIC TRANSFORMATION?

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14. INTERGENERIC HYBRIDISATION BETWEEN CARICA PAPAYA AND WILD VASCONCELLEA SPECIES AND IDENTIFICATION OF A PRSV-P RESISTANCE GENE

15. IDENTIFICATION OF CERTAIN PAPAYA CULTIVARS AND SEX IDENTIFICATION IN PAPAYA BY DNA AMPLIFICATION FINGERPRINTING (DAF)

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16. CHARACTERISTICS OF RIPE PAPAYA STORED AT LOW TEMPERATURE

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17. PRODUCTION OF TRANSGENIC CITRUS PLANTS USING ISOPENTENYLTRANSFERASE (IPT) POSITIVE SELECTION AND REMOVAL OF THE MARKER GENE BY SITE-SPECIFIC RECOMBINATION

A. Ballester | M. Cervera | L. Peña

18. IDENTIFICATION OF AFLP MARKERS ASSOCIATED WITH CITRUS CANKER RESISTANCE WITHIN CITRUS

Y.A. Choi | C. Chen | S. Huang | F.G. Gmitter Jr.

19. AGROBACTERIUM-MEDIATED TRANSFORMATION IN CITRUS USING A NEW GFP VECTOR

20. SUPPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE (INOS) AND CYCLOOXYGENASE II (COX-2) BY FERMENTED PRODUCT OF CITRUS SUNKI PEEL IN LIPOPOLYSACCHARIDE-ACTIVATED RAW264.7 CELLS

21. MICROARRAY EXPRESSION PROFILING OF NAGAMI KUMQUAT IN RESPONSE TO CANKER

A.A. Khalaf | G.A. Moore | F.G. Gmitter Jr.

22. HIGH-THROUGHPUT APPROACHES TO THE IDENTIFICATION OF CITRUS GENES INVOLVED IN FRUIT RESPONSE TO PENICILLIUM DIGITATUM INFECTION

J.F. Marcos | P. Sánchez-Torres | S. Alamar | L. Zacarías | L. González-Candelas

23. PROTOPLAST CO-TRANSFORMATION AND REGENERATION OF TRANSGENIC ‘HAMLIN’ SWEET ORANGE PLANTS CONTAINING A CDNA XA21 XANTHOMONAS RESISTANCE GENE AND GFP

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24. A GFP-CONTAINING BINARY VECTOR FOR AGROBACTERIUM TUMEFACIENS-MEDIATED PLANT TRANSFORMATION

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25. IDENTIFICATION OF ZYGOTIC AND NUCELLAR SEEDLINGS FROM CITRUS ROOTSTOCK CANDIDATES USING RAPD

26. AGROBACTERIUM-MEDIATED TRANSFORMATION OF CITRUS USING TWO BINARY VECTORS

27. AGROBACTERIUM-MEDIATED TRANSFORMATION OF EMBRYOGENIC CULTURES IN ‘MIYAGAWA WASE’ SATSUMA MANDARIN (CITRUS UNSHIU MARC.)

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28. EXPRESSION OF PHI-EA1H PHAGE DEPOLYMERASE GENE WITH CONSTITUTIVE AND INDUCIBLE PROMOTERS, TRANSLATION ENHANCER AND SIGNAL SEQUENCE IN TRANSGENIC APPLE PLANTS INCREASES RESISTANCE TO FIRE BLIGHT

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30. STRATEGIES FOR OBTAINING FIRE BLIGHT RESISTANCE IN APPLE BY RDNA TECHNOLOGY

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31. ALTERNATIVE SELECTION SYSTEMS IN APPLE TRANSFORMATION

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32. MONITORING DIFFERENTIAL EXPRESSION DURING FRUIT MATURATION, RIPENING AND STORAGE AS AN IDENTIFICATION TOOL FOR GENE CANDIDATES FOR SUPERIOR APPLE FRUIT QUALITY

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33. ANALYSIS OF TISSUE UNIFORMITY IN TRANSGENIC APPLE PLANTS

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34. BPMADS4 – A MADS BOX GENE OF BIRCH INDUCES FLOWERS ON TRANSGENIC APPLE PLANTS IN VITRO

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36. GENETIC TRANSFORMATION OF APPLE WITHOUT USE OF A SELECTABLE MARKER

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39. IDENTIFICATION AND EFFECT OF TWO BACTERIAL CONTAMINANTS ON APPLE ORGANOGENESIS

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40. ESTIMATION OF GENE FLOW VIA POLLEN SPREAD FOR THE ORCHARD LAYOUT PRIOR TO THE FIELD RELEASE OF APPLE TRANSFORMANTS

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42. IMPROVEMENT OF ROOTING AND REDUCTION IN PLANT HEIGHT IN APPLE AND PEAR THROUGH GENE TRANSFER

L.H. Zhu | X.Y. Li | M. Nyqvist | M. Welander

43. GENETIC TRANSFORMATION OF APPLE WITH BIBAC VECTORS TO INCREASE SCAB RESISTANCE IN TRANSGENIC LINES

44. CYTOKININ HABITUATION FOR AUTONOMOUS SHOOT INITIATION IN PAWPAW (ASIMINA TRILOBA)

R.L. Geneve | S.T. Kester | K.W. Pomper

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Y. Kanayama | R. Moriguchi | M. Deguchi | K. Kanahama | S. Yamaki

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G.-Q. Song | R.A. Roggers | K.C. Sink | M. Particka | B. Zandstra

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50. MOLECULAR AND PHENOTYPIC CHARACTERIZATION OF SOMACLONAL VARIATION IN DATE PALM OFF-TYPES ORIGINATED FROM TISSUE CULTURE

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53. FICUS CARICA L. PRODUCED BY MICROPROPAGATION

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69. IDENTIFICATION OF RAPD MARKER LINKED TO RESISTANCE OF MUSA TO FUSARIUM OXYSPORUM

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A. Callahan | R. Scorza

74. IMPROVED CONDITIONS FOR AGROBACTERIUM-MEDIATED TRANSFORMATION OF ALMOND

M. Costa | C. Miguel | M.M. Oliveira

75. IMPROVING REGENERATION AND TRANSFORMATION FOR RESISTANCE TO SHARKA IN PRUNUS

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77. RNA INTERFERENCE FOR SHARKA DISEASE RESISTANCE

78. SUSCEPTIBILITY OF ORNAMENTAL PRUNUS TO PLUM POX POTYVIRUS INFECTION

A. Kalinina | D.C.W. Brown | M. Ravelonandro

79. USING MAT VECTOR SYSTEM TO PRODUCE MARKER-FREE TRANSFORMED APRICOT PLANTS

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81. SETTING UP TISSUE CULTURE TECHNIQUES FOR REGENERATION OF APRICOT TRANSGENIC SHOOTS IN A TRANSFORMATION PROGRAM FOR RESISTANCE TO PLUM POX VIRUS

P. Negri | E. Galassi | S. Tartarini | E. Magnanini

82. MOLECULAR GENETIC DISSECTION OF CHILLING INJURY IN PEACH FRUIT

83. ENDOPOLYGALACTURONASE GENOTYPIC VARIATION IN PRUNUS

84. EFFICIENT TRANSFORMATION OF COMMERCIAL APRICOT CULTIVARS AND REGENERATION OF TRANSFORMED PLANTS

C. Petri | S. López-Noguera | N. Alburquerque | L. Burgos

85. THE EFFECT OF CO-EXISTING PRUNUS VIRUSES ON TRANSGENIC PLUM POX VIRUS RESISTANT PLUMS

M. Ravelonandro | J. Kundu | P. Briard | M. Monsion | R. Scorza

86. CONSTRUCTION OF A GENETIC MAP FOR APRICOT WITH MOLECULAR MARKERS AND IDENTIFICATION OF MARKERS ASSOCIATED WITH PLUM POX VIRUS RESISTANCE

J. Salava | J. Polák | B. Krška | D.A. Lalli | A.G. Abbott

87. ADVENTITIOUS SHOOT REGENERATION IN PRUNUS DULCIS – A MOLECULAR APPROACH TO THE REGENERATION PROCESS

88. DEREGULATION OF PLUM POX RESISTANT TRANSGENIC PLUM ‘HONEYSWEET’

89. ISOLATION OF FULL-LENGTH CDNAS WITH PUTATIVE FUNCTIONS IN ALMOND (PRUNUS DULCIS MILL.) FLOWERING

C. Silva | A.M. Sanchez | M.M. Oliveira

90. TRANSFORMATION OF CHERRY: PRUNUS CERASUS L. ‘MONTMORENCY’ AND PRUNUS CERASUS × P. CANESCEN ‘GISELA 6’ MEDIATED BY AGROBACTERIUM TUMEFACIENS AND A TWO-STEP SELECTION SYSTEM

G.-Q. Song | K.C. Sink

91. THE INFLUENCE OF GENOTYPE ON THE INDUCTION OF SOMATIC EMBRYOS FROM IN VITRO CULTURED ZYGOTIC EMBRYOS AND ADVENTITIOUS SHOOT REGENERATION FROM COTYLEDONS OF PEACH AND NECTARINE

C. Srinivasan | R. Scorza

92. SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM IMMATURE COTYLEDONS OF PRUNUS MUME ‘NANKO’

93. SEROLOGICAL AND MOLECULAR VARIABILITY OF PLUM POX VIRUS IN TRANSGENIC AND CONVENTIONAL PLUMS

94. EXPLOITATION OF STRUCTURAL AND FUNCTIONAL GENOMICS DATABASES FOR GENE IDENTIFICATION IN PEACH

95. GENETIC IMPROVEMENT OF ASEXUALLY PROPAGATED PLANTS

D. Infante | M. Osorio | S. Molina | G. Gonzalez

96. GENETIC DIVERSITY OF VENEZUELAN CASSAVA COLLECTION

97. ROOTING AND ACCLIMATIZATION OF CASSAVA (MANIHOT ESCULENTA) EX VITRO

T.W. Zimmerman | K. Williams | L. Joseph | J. Wiltshire | J.A. Kowalski

98. GENETIC TRANSFORMATION OF EMBRYOGENIC CULTURES AND RECOVERY OF TRANSGENIC PLANTS IN VITIS VINIFERA, VITIS ROTUNDIFOLIA AND VITIS HYBRIDS

99. TRANSGENIC ROOTSTOCK PROTEIN TRANSMISSION IN GRAPEVINES

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H. Huang | J. Lu | W. Hunter

101. ISOLATION AND CHARACTERIZATION OF THE 2S ALBUMIN GENE AND PROMOTER FROM GRAPEVINE

102. TOWARDS IDENTIFICATION, ISOLATION AND CHARACTERIZATION OF DISEASE RESISTANT GENES FROM THE NATIVE NORTH AMERICAN GRAPE SPECIES VITIS SHUTTLEWORTHII

J. Lu | H. Huang | Z. Ren | F. Bradeley | W. Hunter

103. ESTABLISHMENT OF EFFICIENT SOMATIC EMBRYOGENESIS AND REGENERATION IN MUSCADINE GRAPE (VITIS ROTUNDIFOLIA)

J. Lu | X. Xu | Y. Jittayasothorn

104. THE EFFECTS OF SALICYLIC ACID ON DISTRIBUTION OF ¹⁴C-ASSIMILATION AND PHOTOSYNTHESIS IN YOUNG GRAPE PLANTS UNDER HEAT STRESS

L.J. Wang | S.H. Li

105. ISOLATION AND CULTURE OF GRAPE PROTOPLASTS FROM EMBRYOGENIC SUSPENSION CULTURES AND LEAVES OF VITIS VINIFERA AND VITIS ROTUNDIFOLIA

X. Xu | J. Lu | D. Dalling | Y. Jittayasothorn | J.W. Grosser