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Articles

GENETIC TRANSFORMATION OF APPLE WITH BIBAC VECTORS TO INCREASE SCAB RESISTANCE IN TRANSGENIC LINES

Article number

738_43

Pages

361 – 367

Language

English

Abstract

The apple scab resistance derived from Malus floribunda 821 is controlled by a single gene (named Vf). A positional cloning approach was used to identify candidate resistance genes by using a BAC library from cultivar Florina. At least three putative resistance genes, named HcrVfs, were identified at the Vf locus.
Two BACs of the Vf region each containing the Hcrvf2 or Hcrvf4 genes were separately subcloned in a binary BIBAC vector for Agrobacterium-mediated transformation.
Transgenic Gala clones were acclimatized in greenhouse and molecularly characterized.
A set of primers was designed to amplify specifically different T-DNA regions including the HcrVf genes by PCR. A scab resistance evaluation was also done.
Deletions of different parts of the BIBAC vectors were observed in all the transgenic lines, mainly with regard to the HcrVf sequences.
As expected by results of molecular analysis, all the clones were susceptible to scab.

Publication

International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species

Authors

J. Zhu, A. Zhou, S. Granozio, E. Andruccioli, E. Belfanti, M. Barbieri, B.A. Vinatzer, A. Patocchi, S. Tartarini, S. Sansavini

Keywords

Malus × domestica, Venturia inaequalis, HcrVf genes

Full text

https://doi.org/10.17660/ActaHortic.2007.738.43

Online Articles (105)

1. INTEGRATING GENOMICS INTO ROSACEAE FRUIT BREEDING

P. Arús

2. GENETIC MODIFICATIONS IN FLORAL CROPS: RESEARCH TO MARKETPLACE

S. Chandler

3. RECOVERY OF DIFFICULT-TO-REGENERATE SPECIES: THE CYCAD EXAMPLE

V.M. Chávez | S.L. Cabrera | R.E. Litz | P.A. Moon

4. GENOMICS APPROACHES TO UNDERSTANDING RIPENING CONTROL AND FRUIT QUALITY IN TOMATO

J. Giovannoni

5. APPLICATIONS OF SOMATIC HYBRIDIZATION AND CYBRIDIZATION IN SCION AND ROOTSTOCK IMPROVEMENT, WITH FOCUS ON CITRUS

6. OVERCOMING CHALLENGES TO DELIVER TRANSGENIC HORTICULTURAL PRODUCTS TO U.S. AND OVERSEAS MARKETS

K. Kahn | P. Schmeissner | E. Jones | B. Richey | B. Simmons

7. CAN KNOWLEDGE ABOUT THE MOLECULAR BASES OF PLANT DISEASE AND DISEASE RESISTANCE HELP ENGINEERING RESISTANCE IN CROPS?

O. Le Gall

8. COMMERCIALIZATION OF GENETICALLY ENGINEERED PAPAYA: BREEDING PERSPECTIVES

R.M. Manshardt

9. MOLECULAR GENETICS OF WALNUTS – A BREEDER’S PERSPECTIVE

G. McGranahan

10. SITE-SPECIFIC RECOMBINATION FOR PLANT GENETIC ENGINEERING: STRATEGY FOR AGRO-MEDIATED GENE STACKING

D.W. Ow

11. CURRENT CHALLENGES OF TROPICAL TREE CROP IMPROVEMENT: INTEGRATING GENOMICS INTO AN APPLIED CACAO BREEDING PROGRAM

12. USING BIOTECHNOLOGY TO IMPROVE RESISTANCE TO ENVIRONMENTAL STRESS IN FRUIT CROPS: THE IMPORTANCE OF UNDERSTANDING PHYSIOLOGY

M. Wisniewski | C. Bassett | J.L. Norelli | T. Artlip | J. Renaut

13. IS IT POSSIBLE TO INDUCE COLD TOLERANCE IN PAPAYA THROUGH GENETIC TRANSFORMATION?

S.A. Dhekney | R.E. Litz | D. Moraga | A. Yadav

14. INTERGENERIC HYBRIDISATION BETWEEN CARICA PAPAYA AND WILD VASCONCELLEA SPECIES AND IDENTIFICATION OF A PRSV-P RESISTANCE GENE

15. IDENTIFICATION OF CERTAIN PAPAYA CULTIVARS AND SEX IDENTIFICATION IN PAPAYA BY DNA AMPLIFICATION FINGERPRINTING (DAF)

S. Somsri | S. Bussabakornkul

16. CHARACTERISTICS OF RIPE PAPAYA STORED AT LOW TEMPERATURE

C. Wongs-Aree | P. Jitareerat | S. Ketsa

17. PRODUCTION OF TRANSGENIC CITRUS PLANTS USING ISOPENTENYLTRANSFERASE (IPT) POSITIVE SELECTION AND REMOVAL OF THE MARKER GENE BY SITE-SPECIFIC RECOMBINATION

A. Ballester | M. Cervera | L. Peña

18. IDENTIFICATION OF AFLP MARKERS ASSOCIATED WITH CITRUS CANKER RESISTANCE WITHIN CITRUS

Y.A. Choi | C. Chen | S. Huang | F.G. Gmitter Jr.

19. AGROBACTERIUM-MEDIATED TRANSFORMATION IN CITRUS USING A NEW GFP VECTOR

20. SUPPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE (INOS) AND CYCLOOXYGENASE II (COX-2) BY FERMENTED PRODUCT OF CITRUS SUNKI PEEL IN LIPOPOLYSACCHARIDE-ACTIVATED RAW264.7 CELLS

21. MICROARRAY EXPRESSION PROFILING OF NAGAMI KUMQUAT IN RESPONSE TO CANKER

A.A. Khalaf | G.A. Moore | F.G. Gmitter Jr.

22. HIGH-THROUGHPUT APPROACHES TO THE IDENTIFICATION OF CITRUS GENES INVOLVED IN FRUIT RESPONSE TO PENICILLIUM DIGITATUM INFECTION

J.F. Marcos | P. Sánchez-Torres | S. Alamar | L. Zacarías | L. González-Candelas

23. PROTOPLAST CO-TRANSFORMATION AND REGENERATION OF TRANSGENIC ‘HAMLIN’ SWEET ORANGE PLANTS CONTAINING A CDNA XA21 XANTHOMONAS RESISTANCE GENE AND GFP

A.A. Omar | J.W. Grosser

24. A GFP-CONTAINING BINARY VECTOR FOR AGROBACTERIUM TUMEFACIENS-MEDIATED PLANT TRANSFORMATION

V. Orbović | G. Pasquali | J.W. Grosser

25. IDENTIFICATION OF ZYGOTIC AND NUCELLAR SEEDLINGS FROM CITRUS ROOTSTOCK CANDIDATES USING RAPD

26. AGROBACTERIUM-MEDIATED TRANSFORMATION OF CITRUS USING TWO BINARY VECTORS

27. AGROBACTERIUM-MEDIATED TRANSFORMATION OF EMBRYOGENIC CULTURES IN ‘MIYAGAWA WASE’ SATSUMA MANDARIN (CITRUS UNSHIU MARC.)

K.J. Song | S.B. Jin | K.Z. Riu

28. EXPRESSION OF PHI-EA1H PHAGE DEPOLYMERASE GENE WITH CONSTITUTIVE AND INDUCIBLE PROMOTERS, TRANSLATION ENHANCER AND SIGNAL SEQUENCE IN TRANSGENIC APPLE PLANTS INCREASES RESISTANCE TO FIRE BLIGHT

29. FEASIBILITY OF ALTERNATIVE SELECTION METHODS FOR TRANSGENIC APPLE AND PEAR USING THE DETOXIFICATION GENE VR-ERE

30. STRATEGIES FOR OBTAINING FIRE BLIGHT RESISTANCE IN APPLE BY RDNA TECHNOLOGY

E.E. Borejsza-Wysocka | M. Malnoy | J.L. Norelli | S.V. Beer | S.H. He

31. ALTERNATIVE SELECTION SYSTEMS IN APPLE TRANSFORMATION

J. Degenhardt | A. Poppe | L. Rösner | I. Sza

32. MONITORING DIFFERENTIAL EXPRESSION DURING FRUIT MATURATION, RIPENING AND STORAGE AS AN IDENTIFICATION TOOL FOR GENE CANDIDATES FOR SUPERIOR APPLE FRUIT QUALITY

R. Dreesen | J. Keulemans

33. ANALYSIS OF TISSUE UNIFORMITY IN TRANSGENIC APPLE PLANTS

H. Flachowsky | M. Riedel | S. Reim | M.V. Hanke

34. BPMADS4 – A MADS BOX GENE OF BIRCH INDUCES FLOWERS ON TRANSGENIC APPLE PLANTS IN VITRO

H. Flachowsky | M.V. Hanke | A. Elo | T. Sopanen

35. PRELIMINARY RESULTS OF SHOOT REGENERATION FROM LEAF EXPLANTS OF IN VITRO CULTURED SHOOTS OF THE APPLE ROOTSTOCK ‘M.9’

M. Höhnle | G. Weber

36. GENETIC TRANSFORMATION OF APPLE WITHOUT USE OF A SELECTABLE MARKER

37. THE ROLE OF VFA1, VFA2 AND VFA4 GENES IN RESISTANCE TO VENTURIA INAEQUALIS IN TRANSGENIC ‘MCINTOSH’ APPLE PLANTS

M. Malnoy | M. Xu | E.E. Borejsza-Wysocka | S.S. Korban | H.S. Aldwinckle

38. INDUCIBLE DNA PROMOTERS FOR USE IN APPLE

39. IDENTIFICATION AND EFFECT OF TWO BACTERIAL CONTAMINANTS ON APPLE ORGANOGENESIS

M.C. Piagnani | S. Guglielmetti | C. Parini

40. ESTIMATION OF GENE FLOW VIA POLLEN SPREAD FOR THE ORCHARD LAYOUT PRIOR TO THE FIELD RELEASE OF APPLE TRANSFORMANTS

J. Soejima

41. THE EFFECT OF TRANSPORTER GENES ON ZINC STRESS IN APPLE (MALUS × DOMESTICA BORKH.)

42. IMPROVEMENT OF ROOTING AND REDUCTION IN PLANT HEIGHT IN APPLE AND PEAR THROUGH GENE TRANSFER

L.H. Zhu | X.Y. Li | M. Nyqvist | M. Welander

43. GENETIC TRANSFORMATION OF APPLE WITH BIBAC VECTORS TO INCREASE SCAB RESISTANCE IN TRANSGENIC LINES

44. CYTOKININ HABITUATION FOR AUTONOMOUS SHOOT INITIATION IN PAWPAW (ASIMINA TRILOBA)

R.L. Geneve | S.T. Kester | K.W. Pomper

45. EFFECTS OF ENVIRONMENTAL STRESSES AND ABSCISIC ACID ON SORBITOL-6-PHOSPHATE DEHYDROGENASE EXPRESSION IN ROSACEAE FRUIT TREES

Y. Kanayama | R. Moriguchi | M. Deguchi | K. Kanahama | S. Yamaki

46. EVALUATION OF TOLERANCE OF COLLETOTRICHUM ACUTATUM IN STRAWBERRY PLANTS TRANSFORMED WITH TRICHODERMA-DERIVED GENES

47. ANALYSIS OF TELOMERE LENGTHS IN APPLE AND CHERRY TREES

R. Moriguchi | K. Kato | K. Kanahama | Y. Kanayama | H. Kikuchi

48. PRODUCTION OF HERBICIDE-RESISTANT HIGHBUSH BLUEBERRY ‘LEGACY’ BY AGROBACTERIUM-MEDIATED TRANSFORMATION OF THE BAR GENE

G.-Q. Song | R.A. Roggers | K.C. Sink | M. Particka | B. Zandstra

49. SEXUAL POLYPLOIDIZATION OF JAPANESE PERSIMMON BY UTILIZING UNREDUCED EGG

A. Yamada | R. Tao

50. MOLECULAR AND PHENOTYPIC CHARACTERIZATION OF SOMACLONAL VARIATION IN DATE PALM OFF-TYPES ORIGINATED FROM TISSUE CULTURE

51. AGROBACTERIUM RHIZOGENES-MEDIATED TRANSFORMATION OF PASSIONFRUIT SPECIES: PASSIFLORA CINCINNATA AND P. EDULIS F. FLAVICARPA

52. 2,4-D AND KINETIN IN CALLOGENESIS OF FICUS CARICA L.

E.A. Ferreira | M. Pasqual | J.C. Rezende

53. FICUS CARICA L. PRODUCED BY MICROPROPAGATION

E.A. Ferreira | M. Pasqual

54. SOMATIC EMBRYOGENESIS AND PLANTLET REGENERATION OF MANGO (MANGIFERA INDICA L.)

C.P. Flórez-Ramos | M.E. Buitrago | Z. Lentini | J. Cock

55. PLANT RECOVERY FOLLOWING TRANSFORMATION OF AVOCADO WITH ANTI-FUNGAL PROTEIN AND SAM HYDROLASE GENES

R.E. Litz | S.H.T. Raharjo | D. Efendi | Witjaksono | M.A. Gómez-Lim

56. EXPLORING THE MECHANISM OF POTASSIUM CHLORATE-INDUCED FLOWERING IN DIMOCARPUS LONGAN

T.K. Matsumoto | T. Tsumura | F. Zee

57. TRACING THE GENETIC BASE OF CHERIMOYA (ANNONA CHERIMOLA) COMMERCIAL CULTIVARS THROUGH AFLP ANALYSIS OF DIVERSITY AT THE SPECIES’ PUTATIVE CENTER OF ORIGIN

A. Narváez-Trujillo | J.M. Barreiro | R. Morales Astudillo

58. AGROBACTERIUM-MEDIATED TRANSFORMATION OF ANACARDIUM OCCIDENTALE L. (CASHEW) USING GFP MARKER SYSTEM

59. GENETIC TRANSFORMATION OF OLIVE SOMATIC EMBRYOS THROUGH BIOLISTICS

G. Pérez-Barranco | J.A. Mercado | F. Pliego-Alfaro | C. Sánchez-Romero

60. INTELLECTUAL PROPERTY RIGHTS AND REGULATORY ISSUES RELATED TO BIOTECHNOLOGY OF TROPICAL SPECIES IN INDIA

J. Prakash

61. CLONAL REGENERATION OF LITCHI (LITCHI CHINENSIS SONN.) VIA SOMATIC EMBRYOGENESIS

S.H.T. Raharjo | R.E. Litz

62. IN VITRO PROPAGATION AND REGENERATION OF SOLANUM QUITOENSE (LULO) PLANTS AND THEIR USE AS ELITE CLONES BY RESOURCE FARMERS

63. RESISTANCE TO PASSION FRUIT WOODINESS VIRUS IN TRANSGENIC PLANTS OF THE YELLOW PASSION FRUIT EXPRESSING THE VIRAL COAT PROTEIN GENE

64. IN VITRO CALLUS INITIATION OF GUAVA

G. Yang | Z. Lu

65. TRANSGENIC PLANTAIN (CV. ‘HARTON’) PLANTS RESISTANT TO HERBICIDE BASTA OBTAINED BY ELECTROPORATION

E. de García | C. Villarroel

66. CHARACTERIZATION OF RIPENING-SPECIFIC MADS-BOX GENES FROM BANANA

67. FLOWERING TIME GENE ANALOGUES IN MUSA

C. Giménez | M. Colmenares | Y. Hernández | M.A. Gómez-Lim

68. INSTABILITY OF TRANSGENES IN IN VITRO-MAINTAINED BANANA PLANTS

K. Matsumoto | N.B. Cunha | L.S. Morais | F.J.L. Aragão

69. IDENTIFICATION OF RAPD MARKER LINKED TO RESISTANCE OF MUSA TO FUSARIUM OXYSPORUM

70. PROLONGING THE LONGEVITY OF CYMBIDIUM FLOWERS THROUGH GENETIC TRANSFORMATION

71. BIOTECHNOLOGY FOR FLOWER DEVELOPMENT AND PIGMENT PRODUCTION IN TAGETES ERECTA

72. SEQUENCING AND ANNOTATION OF THE EVERGROWING LOCUS FROM WILD-TYPE AND MUTANT GENOMES REVEALS SEVERAL CANDIDATE GENES FOR THE CONTROL OF TERMINAL BUD FORMATION IN RESPONSE TO DORMANCY INDUCING CONDITIONS

D.G. Bielenberg | S. Fan | G.L. Reighard | A.G. Abbott

73. EFFECTS OF A PEACH ANTISENSE ACC OXIDASE GENE ON PLUM FRUIT QUALITY

A. Callahan | R. Scorza

74. IMPROVED CONDITIONS FOR AGROBACTERIUM-MEDIATED TRANSFORMATION OF ALMOND

M. Costa | C. Miguel | M.M. Oliveira

75. IMPROVING REGENERATION AND TRANSFORMATION FOR RESISTANCE TO SHARKA IN PRUNUS

76. BEHAVIOUR OF AGROBACTERIUM RHIZOGENES-MEDIATED TRANSFORMANTS OF ‘INMIL’ (P. INCISA × SERRULA) CHERRY ROOTSTOCK

PH. Druart | R. Gruselle

77. RNA INTERFERENCE FOR SHARKA DISEASE RESISTANCE

78. SUSCEPTIBILITY OF ORNAMENTAL PRUNUS TO PLUM POX POTYVIRUS INFECTION

A. Kalinina | D.C.W. Brown | M. Ravelonandro

79. USING MAT VECTOR SYSTEM TO PRODUCE MARKER-FREE TRANSFORMED APRICOT PLANTS

S. López-Noguera | C. Petri | L. Burgos

80. TRANSGENIC PLUM (PRUNUS DOMESTICA L.) PLANTS OBTAINED BY AGROBACTERIUM-MEDIATED TRANSFORMATION OF LEAF EXPLANTS WITH VARIOUS SELECTIVE AGENTS

R.V. Mikhailov | S.V. Dolgov

81. SETTING UP TISSUE CULTURE TECHNIQUES FOR REGENERATION OF APRICOT TRANSGENIC SHOOTS IN A TRANSFORMATION PROGRAM FOR RESISTANCE TO PLUM POX VIRUS

P. Negri | E. Galassi | S. Tartarini | E. Magnanini

82. MOLECULAR GENETIC DISSECTION OF CHILLING INJURY IN PEACH FRUIT

83. ENDOPOLYGALACTURONASE GENOTYPIC VARIATION IN PRUNUS

84. EFFICIENT TRANSFORMATION OF COMMERCIAL APRICOT CULTIVARS AND REGENERATION OF TRANSFORMED PLANTS

C. Petri | S. López-Noguera | N. Alburquerque | L. Burgos

85. THE EFFECT OF CO-EXISTING PRUNUS VIRUSES ON TRANSGENIC PLUM POX VIRUS RESISTANT PLUMS

M. Ravelonandro | J. Kundu | P. Briard | M. Monsion | R. Scorza

86. CONSTRUCTION OF A GENETIC MAP FOR APRICOT WITH MOLECULAR MARKERS AND IDENTIFICATION OF MARKERS ASSOCIATED WITH PLUM POX VIRUS RESISTANCE

J. Salava | J. Polák | B. Krška | D.A. Lalli | A.G. Abbott

87. ADVENTITIOUS SHOOT REGENERATION IN PRUNUS DULCIS – A MOLECULAR APPROACH TO THE REGENERATION PROCESS

88. DEREGULATION OF PLUM POX RESISTANT TRANSGENIC PLUM ‘HONEYSWEET’

89. ISOLATION OF FULL-LENGTH CDNAS WITH PUTATIVE FUNCTIONS IN ALMOND (PRUNUS DULCIS MILL.) FLOWERING

C. Silva | A.M. Sanchez | M.M. Oliveira

90. TRANSFORMATION OF CHERRY: PRUNUS CERASUS L. ‘MONTMORENCY’ AND PRUNUS CERASUS × P. CANESCEN ‘GISELA 6’ MEDIATED BY AGROBACTERIUM TUMEFACIENS AND A TWO-STEP SELECTION SYSTEM

G.-Q. Song | K.C. Sink

91. THE INFLUENCE OF GENOTYPE ON THE INDUCTION OF SOMATIC EMBRYOS FROM IN VITRO CULTURED ZYGOTIC EMBRYOS AND ADVENTITIOUS SHOOT REGENERATION FROM COTYLEDONS OF PEACH AND NECTARINE

C. Srinivasan | R. Scorza

92. SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM IMMATURE COTYLEDONS OF PRUNUS MUME ‘NANKO’

93. SEROLOGICAL AND MOLECULAR VARIABILITY OF PLUM POX VIRUS IN TRANSGENIC AND CONVENTIONAL PLUMS

94. EXPLOITATION OF STRUCTURAL AND FUNCTIONAL GENOMICS DATABASES FOR GENE IDENTIFICATION IN PEACH

95. GENETIC IMPROVEMENT OF ASEXUALLY PROPAGATED PLANTS

D. Infante | M. Osorio | S. Molina | G. Gonzalez

96. GENETIC DIVERSITY OF VENEZUELAN CASSAVA COLLECTION

97. ROOTING AND ACCLIMATIZATION OF CASSAVA (MANIHOT ESCULENTA) EX VITRO

T.W. Zimmerman | K. Williams | L. Joseph | J. Wiltshire | J.A. Kowalski

98. GENETIC TRANSFORMATION OF EMBRYOGENIC CULTURES AND RECOVERY OF TRANSGENIC PLANTS IN VITIS VINIFERA, VITIS ROTUNDIFOLIA AND VITIS HYBRIDS

99. TRANSGENIC ROOTSTOCK PROTEIN TRANSMISSION IN GRAPEVINES

100. COMPARATIVE ANALYSIS OF STILBENE SYNTHASE GENES AMONG VITIS SPECIES

H. Huang | J. Lu | W. Hunter

101. ISOLATION AND CHARACTERIZATION OF THE 2S ALBUMIN GENE AND PROMOTER FROM GRAPEVINE

102. TOWARDS IDENTIFICATION, ISOLATION AND CHARACTERIZATION OF DISEASE RESISTANT GENES FROM THE NATIVE NORTH AMERICAN GRAPE SPECIES VITIS SHUTTLEWORTHII

J. Lu | H. Huang | Z. Ren | F. Bradeley | W. Hunter

103. ESTABLISHMENT OF EFFICIENT SOMATIC EMBRYOGENESIS AND REGENERATION IN MUSCADINE GRAPE (VITIS ROTUNDIFOLIA)

J. Lu | X. Xu | Y. Jittayasothorn

104. THE EFFECTS OF SALICYLIC ACID ON DISTRIBUTION OF ¹⁴C-ASSIMILATION AND PHOTOSYNTHESIS IN YOUNG GRAPE PLANTS UNDER HEAT STRESS

L.J. Wang | S.H. Li

105. ISOLATION AND CULTURE OF GRAPE PROTOPLASTS FROM EMBRYOGENIC SUSPENSION CULTURES AND LEAVES OF VITIS VINIFERA AND VITIS ROTUNDIFOLIA

X. Xu | J. Lu | D. Dalling | Y. Jittayasothorn | J.W. Grosser