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Articles

ROOTING AND ACCLIMATIZATION OF CASSAVA (MANIHOT ESCULENTA) EX VITRO

Article number

738_97

Pages

735 – 740

Language

English

Abstract

Micropropagation of cassava is relatively straightforward and shoots from microcuttings can root without plant growth regulators in vitro.
However, acclimatizing in vitro rooted shoots can result in high mortality.
Cassava roots are prone to deterioration following wounding or bruising causing great losses during acclimatization.
Direct rooting was conducted of cassava shoots pretreated by growing them in vitro on 0% to 10% sucrose.
Shoots, 1.5 cm in length from the varying sucrose media were rooted in vitro in medium grade vermiculite or in situ in ProMix potting medium.
Sucrose levels > 6% caused shorter plants than the other treatments.
Shoots rooted equally well with both media.
A success rate of > 95% was obtained following rooting in vermiculite followed by transfer and acclimatization in a ProMix potting medium.
Vermiculite was easily removed from cassava roots reducing the damage normally occurring during the rinsing and removal of adhering gelled medium.
In vitro rooting of cassava in vermiculite increases survival rates during acclimatization.
Direct rooting in pots was most successful from shoots grown on 4% to 8% sucrose.

Publication

International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species

Authors

T.W. Zimmerman, K. Williams, L. Joseph, J. Wiltshire, J.A. Kowalski

Keywords

sucrose, starch accumulation, in vitro, in situ

Full text

https://doi.org/10.17660/ActaHortic.2007.738.97

Online Articles (105)

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2. GENETIC MODIFICATIONS IN FLORAL CROPS: RESEARCH TO MARKETPLACE

S. Chandler

3. RECOVERY OF DIFFICULT-TO-REGENERATE SPECIES: THE CYCAD EXAMPLE

V.M. Chávez | S.L. Cabrera | R.E. Litz | P.A. Moon

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5. APPLICATIONS OF SOMATIC HYBRIDIZATION AND CYBRIDIZATION IN SCION AND ROOTSTOCK IMPROVEMENT, WITH FOCUS ON CITRUS

6. OVERCOMING CHALLENGES TO DELIVER TRANSGENIC HORTICULTURAL PRODUCTS TO U.S. AND OVERSEAS MARKETS

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7. CAN KNOWLEDGE ABOUT THE MOLECULAR BASES OF PLANT DISEASE AND DISEASE RESISTANCE HELP ENGINEERING RESISTANCE IN CROPS?

O. Le Gall

8. COMMERCIALIZATION OF GENETICALLY ENGINEERED PAPAYA: BREEDING PERSPECTIVES

R.M. Manshardt

9. MOLECULAR GENETICS OF WALNUTS – A BREEDER’S PERSPECTIVE

G. McGranahan

10. SITE-SPECIFIC RECOMBINATION FOR PLANT GENETIC ENGINEERING: STRATEGY FOR AGRO-MEDIATED GENE STACKING

D.W. Ow

11. CURRENT CHALLENGES OF TROPICAL TREE CROP IMPROVEMENT: INTEGRATING GENOMICS INTO AN APPLIED CACAO BREEDING PROGRAM

12. USING BIOTECHNOLOGY TO IMPROVE RESISTANCE TO ENVIRONMENTAL STRESS IN FRUIT CROPS: THE IMPORTANCE OF UNDERSTANDING PHYSIOLOGY

M. Wisniewski | C. Bassett | J.L. Norelli | T. Artlip | J. Renaut

13. IS IT POSSIBLE TO INDUCE COLD TOLERANCE IN PAPAYA THROUGH GENETIC TRANSFORMATION?

S.A. Dhekney | R.E. Litz | D. Moraga | A. Yadav

14. INTERGENERIC HYBRIDISATION BETWEEN CARICA PAPAYA AND WILD VASCONCELLEA SPECIES AND IDENTIFICATION OF A PRSV-P RESISTANCE GENE

15. IDENTIFICATION OF CERTAIN PAPAYA CULTIVARS AND SEX IDENTIFICATION IN PAPAYA BY DNA AMPLIFICATION FINGERPRINTING (DAF)

S. Somsri | S. Bussabakornkul

16. CHARACTERISTICS OF RIPE PAPAYA STORED AT LOW TEMPERATURE

C. Wongs-Aree | P. Jitareerat | S. Ketsa

17. PRODUCTION OF TRANSGENIC CITRUS PLANTS USING ISOPENTENYLTRANSFERASE (IPT) POSITIVE SELECTION AND REMOVAL OF THE MARKER GENE BY SITE-SPECIFIC RECOMBINATION

A. Ballester | M. Cervera | L. Peña

18. IDENTIFICATION OF AFLP MARKERS ASSOCIATED WITH CITRUS CANKER RESISTANCE WITHIN CITRUS

Y.A. Choi | C. Chen | S. Huang | F.G. Gmitter Jr.

19. AGROBACTERIUM-MEDIATED TRANSFORMATION IN CITRUS USING A NEW GFP VECTOR

20. SUPPRESSION OF INDUCIBLE NITRIC OXIDE SYNTHASE (INOS) AND CYCLOOXYGENASE II (COX-2) BY FERMENTED PRODUCT OF CITRUS SUNKI PEEL IN LIPOPOLYSACCHARIDE-ACTIVATED RAW264.7 CELLS

21. MICROARRAY EXPRESSION PROFILING OF NAGAMI KUMQUAT IN RESPONSE TO CANKER

A.A. Khalaf | G.A. Moore | F.G. Gmitter Jr.

22. HIGH-THROUGHPUT APPROACHES TO THE IDENTIFICATION OF CITRUS GENES INVOLVED IN FRUIT RESPONSE TO PENICILLIUM DIGITATUM INFECTION

J.F. Marcos | P. Sánchez-Torres | S. Alamar | L. Zacarías | L. González-Candelas

23. PROTOPLAST CO-TRANSFORMATION AND REGENERATION OF TRANSGENIC ‘HAMLIN’ SWEET ORANGE PLANTS CONTAINING A CDNA XA21 XANTHOMONAS RESISTANCE GENE AND GFP

A.A. Omar | J.W. Grosser

24. A GFP-CONTAINING BINARY VECTOR FOR AGROBACTERIUM TUMEFACIENS-MEDIATED PLANT TRANSFORMATION

V. Orbović | G. Pasquali | J.W. Grosser

25. IDENTIFICATION OF ZYGOTIC AND NUCELLAR SEEDLINGS FROM CITRUS ROOTSTOCK CANDIDATES USING RAPD

26. AGROBACTERIUM-MEDIATED TRANSFORMATION OF CITRUS USING TWO BINARY VECTORS

27. AGROBACTERIUM-MEDIATED TRANSFORMATION OF EMBRYOGENIC CULTURES IN ‘MIYAGAWA WASE’ SATSUMA MANDARIN (CITRUS UNSHIU MARC.)

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28. EXPRESSION OF PHI-EA1H PHAGE DEPOLYMERASE GENE WITH CONSTITUTIVE AND INDUCIBLE PROMOTERS, TRANSLATION ENHANCER AND SIGNAL SEQUENCE IN TRANSGENIC APPLE PLANTS INCREASES RESISTANCE TO FIRE BLIGHT

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E.E. Borejsza-Wysocka | M. Malnoy | J.L. Norelli | S.V. Beer | S.H. He

31. ALTERNATIVE SELECTION SYSTEMS IN APPLE TRANSFORMATION

J. Degenhardt | A. Poppe | L. Rösner | I. Sza

32. MONITORING DIFFERENTIAL EXPRESSION DURING FRUIT MATURATION, RIPENING AND STORAGE AS AN IDENTIFICATION TOOL FOR GENE CANDIDATES FOR SUPERIOR APPLE FRUIT QUALITY

R. Dreesen | J. Keulemans

33. ANALYSIS OF TISSUE UNIFORMITY IN TRANSGENIC APPLE PLANTS

H. Flachowsky | M. Riedel | S. Reim | M.V. Hanke

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M. Malnoy | M. Xu | E.E. Borejsza-Wysocka | S.S. Korban | H.S. Aldwinckle

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39. IDENTIFICATION AND EFFECT OF TWO BACTERIAL CONTAMINANTS ON APPLE ORGANOGENESIS

M.C. Piagnani | S. Guglielmetti | C. Parini

40. ESTIMATION OF GENE FLOW VIA POLLEN SPREAD FOR THE ORCHARD LAYOUT PRIOR TO THE FIELD RELEASE OF APPLE TRANSFORMANTS

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42. IMPROVEMENT OF ROOTING AND REDUCTION IN PLANT HEIGHT IN APPLE AND PEAR THROUGH GENE TRANSFER

L.H. Zhu | X.Y. Li | M. Nyqvist | M. Welander

43. GENETIC TRANSFORMATION OF APPLE WITH BIBAC VECTORS TO INCREASE SCAB RESISTANCE IN TRANSGENIC LINES

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R.L. Geneve | S.T. Kester | K.W. Pomper

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Y. Kanayama | R. Moriguchi | M. Deguchi | K. Kanahama | S. Yamaki

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47. ANALYSIS OF TELOMERE LENGTHS IN APPLE AND CHERRY TREES

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G.-Q. Song | R.A. Roggers | K.C. Sink | M. Particka | B. Zandstra

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A. Yamada | R. Tao

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52. 2,4-D AND KINETIN IN CALLOGENESIS OF FICUS CARICA L.

E.A. Ferreira | M. Pasqual | J.C. Rezende

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C.P. Flórez-Ramos | M.E. Buitrago | Z. Lentini | J. Cock

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R.E. Litz | S.H.T. Raharjo | D. Efendi | Witjaksono | M.A. Gómez-Lim

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S.H.T. Raharjo | R.E. Litz

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E. de García | C. Villarroel

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68. INSTABILITY OF TRANSGENES IN IN VITRO-MAINTAINED BANANA PLANTS

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69. IDENTIFICATION OF RAPD MARKER LINKED TO RESISTANCE OF MUSA TO FUSARIUM OXYSPORUM

70. PROLONGING THE LONGEVITY OF CYMBIDIUM FLOWERS THROUGH GENETIC TRANSFORMATION

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A. Callahan | R. Scorza

74. IMPROVED CONDITIONS FOR AGROBACTERIUM-MEDIATED TRANSFORMATION OF ALMOND

M. Costa | C. Miguel | M.M. Oliveira

75. IMPROVING REGENERATION AND TRANSFORMATION FOR RESISTANCE TO SHARKA IN PRUNUS

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PH. Druart | R. Gruselle

77. RNA INTERFERENCE FOR SHARKA DISEASE RESISTANCE

78. SUSCEPTIBILITY OF ORNAMENTAL PRUNUS TO PLUM POX POTYVIRUS INFECTION

A. Kalinina | D.C.W. Brown | M. Ravelonandro

79. USING MAT VECTOR SYSTEM TO PRODUCE MARKER-FREE TRANSFORMED APRICOT PLANTS

S. López-Noguera | C. Petri | L. Burgos

80. TRANSGENIC PLUM (PRUNUS DOMESTICA L.) PLANTS OBTAINED BY AGROBACTERIUM-MEDIATED TRANSFORMATION OF LEAF EXPLANTS WITH VARIOUS SELECTIVE AGENTS

R.V. Mikhailov | S.V. Dolgov

81. SETTING UP TISSUE CULTURE TECHNIQUES FOR REGENERATION OF APRICOT TRANSGENIC SHOOTS IN A TRANSFORMATION PROGRAM FOR RESISTANCE TO PLUM POX VIRUS

P. Negri | E. Galassi | S. Tartarini | E. Magnanini

82. MOLECULAR GENETIC DISSECTION OF CHILLING INJURY IN PEACH FRUIT

83. ENDOPOLYGALACTURONASE GENOTYPIC VARIATION IN PRUNUS

84. EFFICIENT TRANSFORMATION OF COMMERCIAL APRICOT CULTIVARS AND REGENERATION OF TRANSFORMED PLANTS

C. Petri | S. López-Noguera | N. Alburquerque | L. Burgos

85. THE EFFECT OF CO-EXISTING PRUNUS VIRUSES ON TRANSGENIC PLUM POX VIRUS RESISTANT PLUMS

M. Ravelonandro | J. Kundu | P. Briard | M. Monsion | R. Scorza

86. CONSTRUCTION OF A GENETIC MAP FOR APRICOT WITH MOLECULAR MARKERS AND IDENTIFICATION OF MARKERS ASSOCIATED WITH PLUM POX VIRUS RESISTANCE

J. Salava | J. Polák | B. Krška | D.A. Lalli | A.G. Abbott

87. ADVENTITIOUS SHOOT REGENERATION IN PRUNUS DULCIS – A MOLECULAR APPROACH TO THE REGENERATION PROCESS

88. DEREGULATION OF PLUM POX RESISTANT TRANSGENIC PLUM ‘HONEYSWEET’

89. ISOLATION OF FULL-LENGTH CDNAS WITH PUTATIVE FUNCTIONS IN ALMOND (PRUNUS DULCIS MILL.) FLOWERING

C. Silva | A.M. Sanchez | M.M. Oliveira

90. TRANSFORMATION OF CHERRY: PRUNUS CERASUS L. ‘MONTMORENCY’ AND PRUNUS CERASUS × P. CANESCEN ‘GISELA 6’ MEDIATED BY AGROBACTERIUM TUMEFACIENS AND A TWO-STEP SELECTION SYSTEM

G.-Q. Song | K.C. Sink

91. THE INFLUENCE OF GENOTYPE ON THE INDUCTION OF SOMATIC EMBRYOS FROM IN VITRO CULTURED ZYGOTIC EMBRYOS AND ADVENTITIOUS SHOOT REGENERATION FROM COTYLEDONS OF PEACH AND NECTARINE

C. Srinivasan | R. Scorza

92. SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM IMMATURE COTYLEDONS OF PRUNUS MUME ‘NANKO’

93. SEROLOGICAL AND MOLECULAR VARIABILITY OF PLUM POX VIRUS IN TRANSGENIC AND CONVENTIONAL PLUMS

94. EXPLOITATION OF STRUCTURAL AND FUNCTIONAL GENOMICS DATABASES FOR GENE IDENTIFICATION IN PEACH

95. GENETIC IMPROVEMENT OF ASEXUALLY PROPAGATED PLANTS

D. Infante | M. Osorio | S. Molina | G. Gonzalez

96. GENETIC DIVERSITY OF VENEZUELAN CASSAVA COLLECTION

97. ROOTING AND ACCLIMATIZATION OF CASSAVA (MANIHOT ESCULENTA) EX VITRO

T.W. Zimmerman | K. Williams | L. Joseph | J. Wiltshire | J.A. Kowalski

98. GENETIC TRANSFORMATION OF EMBRYOGENIC CULTURES AND RECOVERY OF TRANSGENIC PLANTS IN VITIS VINIFERA, VITIS ROTUNDIFOLIA AND VITIS HYBRIDS

99. TRANSGENIC ROOTSTOCK PROTEIN TRANSMISSION IN GRAPEVINES

100. COMPARATIVE ANALYSIS OF STILBENE SYNTHASE GENES AMONG VITIS SPECIES

H. Huang | J. Lu | W. Hunter

101. ISOLATION AND CHARACTERIZATION OF THE 2S ALBUMIN GENE AND PROMOTER FROM GRAPEVINE

102. TOWARDS IDENTIFICATION, ISOLATION AND CHARACTERIZATION OF DISEASE RESISTANT GENES FROM THE NATIVE NORTH AMERICAN GRAPE SPECIES VITIS SHUTTLEWORTHII

J. Lu | H. Huang | Z. Ren | F. Bradeley | W. Hunter

103. ESTABLISHMENT OF EFFICIENT SOMATIC EMBRYOGENESIS AND REGENERATION IN MUSCADINE GRAPE (VITIS ROTUNDIFOLIA)

J. Lu | X. Xu | Y. Jittayasothorn

104. THE EFFECTS OF SALICYLIC ACID ON DISTRIBUTION OF ¹⁴C-ASSIMILATION AND PHOTOSYNTHESIS IN YOUNG GRAPE PLANTS UNDER HEAT STRESS

L.J. Wang | S.H. Li

105. ISOLATION AND CULTURE OF GRAPE PROTOPLASTS FROM EMBRYOGENIC SUSPENSION CULTURES AND LEAVES OF VITIS VINIFERA AND VITIS ROTUNDIFOLIA

X. Xu | J. Lu | D. Dalling | Y. Jittayasothorn | J.W. Grosser