Articles
SOMATIC EMBRYOGENESIS AND PLANTLET REGENERATION OF MANGO (MANGIFERA INDICA L.)
Article number
738_54
Pages
443 – 445
Language
English
Abstract
Flowering and its control is one of the most important development aspects of crop plants.
There are still many crop plants in which control of flowering is not optimal.
Opportune flowering could greatly increase fruit crop productivity and income generation through a better synchronization of fruit production with market demand.
In the tropics, fruits can be produced throughout the year; nevertheless fruit producers face major problems with the seasonality of their production due to uncontrolled flowering and the consequent difficulties in balancing supply and demand of high quality fruits.
Mangos are grown worldwide in the tropics, are nutritionally important and also an attractive option to increase incomes and reduce poverty in the rural sector of developing countries.
The principal objective of this project is to manipulate the expression of a target set of developmental genes in mango that are known to modulate flowering.
To apply these tools, a consistent embryogenesis and regeneration protocol is required.
CIAT is adapting and optimizing a protocol to induce somatic embryogenesis from Colombian and Florida mango cultivars, as a first step to target a broader range of genotypes.
Nucellar tissues from immature fruits are used as starting explant.
At present, best results are obtained with Keitt cultivar, reaching 48% of embryogenic culture induction.
Embryos in different developmental stages have been observed.
Proliferation and regeneration assays are in progress.
There are still many crop plants in which control of flowering is not optimal.
Opportune flowering could greatly increase fruit crop productivity and income generation through a better synchronization of fruit production with market demand.
In the tropics, fruits can be produced throughout the year; nevertheless fruit producers face major problems with the seasonality of their production due to uncontrolled flowering and the consequent difficulties in balancing supply and demand of high quality fruits.
Mangos are grown worldwide in the tropics, are nutritionally important and also an attractive option to increase incomes and reduce poverty in the rural sector of developing countries.
The principal objective of this project is to manipulate the expression of a target set of developmental genes in mango that are known to modulate flowering.
To apply these tools, a consistent embryogenesis and regeneration protocol is required.
CIAT is adapting and optimizing a protocol to induce somatic embryogenesis from Colombian and Florida mango cultivars, as a first step to target a broader range of genotypes.
Nucellar tissues from immature fruits are used as starting explant.
At present, best results are obtained with Keitt cultivar, reaching 48% of embryogenic culture induction.
Embryos in different developmental stages have been observed.
Proliferation and regeneration assays are in progress.
Authors
C.P. Flórez-Ramos, M.E. Buitrago, Z. Lentini, J. Cock
Keywords
nucellus, proembryogenic masses, somatic embryo, induction, tissue culture
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