Articles
INFLUENCE OF EXPLANT SELECTION AND CULTURE CONDITIONS ON ORGANOGENESIS AND GERMPLASM CONSERVATION IN BACOPA MONNIERI (L.) WETTST.
Article number
756_13
Pages
119 – 128
Language
English
Abstract
Investigations were conducted to optimize in vitro culture conditions for multiple shoot induction and use of nodal segments for germplasm conservation in Bacopa monnieri. Explants derived from leaf and internode tissues of the field-grown and in vitro cultured plants were cultured on the MS medium supplemented with four concentrations and combination of 6-benzylaminopurine (BAP) and 2,4-dichlorophenoxyacetic (2,4-D) plant growth regulators.
Sucrose (30 g/L) and agar (7 g/L) were kept constant in all experiments.
The conditions investigated for the total number of shoots produced were the number of explants from one leaf in transverse and longitudinal plane, wounding/incisions on explants, and the effect of plant growth regulators.
Internode explants were used as either whole with incisions or cut into two halves longitudinally and wounded.
Highest number of shoot regeneration occurred from leaf explants which were cut in half and cultured in medium containing 0.5 mg/L BAP + 0.5 mg/L 2,4-D under 16 h photoperiod.
Shoot induction occurred only at the cut / wounded surfaces of explants.
Furthermore, the optimum shoot regeneration from internode explants also occurred at 0.5 mg/L BAP + 0.5 mg/L 2,4-D, but when the internode explants were kept intact and wounded followed by culturing under 16 h light.
Preliminary studies suggest that nodal explants can be used for germplasm conservation/transportation purpose by encapsulating them in sodium alginate.
Sucrose (30 g/L) and agar (7 g/L) were kept constant in all experiments.
The conditions investigated for the total number of shoots produced were the number of explants from one leaf in transverse and longitudinal plane, wounding/incisions on explants, and the effect of plant growth regulators.
Internode explants were used as either whole with incisions or cut into two halves longitudinally and wounded.
Highest number of shoot regeneration occurred from leaf explants which were cut in half and cultured in medium containing 0.5 mg/L BAP + 0.5 mg/L 2,4-D under 16 h photoperiod.
Shoot induction occurred only at the cut / wounded surfaces of explants.
Furthermore, the optimum shoot regeneration from internode explants also occurred at 0.5 mg/L BAP + 0.5 mg/L 2,4-D, but when the internode explants were kept intact and wounded followed by culturing under 16 h light.
Preliminary studies suggest that nodal explants can be used for germplasm conservation/transportation purpose by encapsulating them in sodium alginate.
Authors
N. Joshee, D. Harris, A. Yadav, A.K. Yadav
Keywords
explant wounding, encapsulation, regeneration, synseeds, medicinal plant, shoot regeneration
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