Articles
IN VITRO CULTURE OF EPIPREMNUM AUREUM, SYNGONIUM PODOPHYLLUM, AND LONICERA MACRANTHODES, THREE IMPORTANT MEDICINAL PLANTS
Article number
756_17
Pages
155 – 162
Language
English
Abstract
Simple and effective regeneration methods were established for propagating Epipremnum aureum Golden Pothos, Syngonium podophyllum Variegatum, and Lonicera macranthodes Jincuilei, three important medicinal plants.
Somatic embryos formed directly at the cut edges or on the surface of petiole explants of Epipremnum cultured on MS medium supplemented with TDZ (N-phenyl-N-1,2,3-thiadiazol-5-ylurea) and NAA (α-naphthalene acetic acid) or TDZ and 2,4-D (2,4-dichlorophenoxyacetic acid). The frequency of explants with somatic embryo formation ranged from 70 to 95%. Embryos matured and germinated into plantlets.
The frequency of explants with embryo germination varied from 60 to 90%. On average, each explant was able to produce 60 plantlets.
Somatic embryos also occurred directly on the petiole explant surface of Syngonium cultured on MS medium supplemented with TDZ and NAA or TDZ and 2,4-D. The frequencies of petiole explants with somatic embryo occurrence and embryo germination were high, up to 85% and 80%, respectively.
Somatic embryos were able to germinate on the initial induction medium and after transferring onto MS medium containing BA (6-benzylaminopurine) and NAA. Fifty to 150 plantlets were regenerated from a single petiole explant. Lonicera was regenerated via indirect shoot organogenesis as calli formed from leaf explants cultured on B5 medium containing 2,4-D, BA, and NAA. Adventitious shoot occurred in calli cultured on B5 medium supplemented with BA, NAA, and kinetin.
Shoot rooted well on MS medium containing IBA. All regenerated plantlets grew vigorously after transplanting to a soilless container substrate in a shaded greenhouse.
These regeneration systems we have established will provide new means for propagation of pathogen-free, uniform plants and can potentially be scale-up for mass multiplication using bioreactors.
Somatic embryos formed directly at the cut edges or on the surface of petiole explants of Epipremnum cultured on MS medium supplemented with TDZ (N-phenyl-N-1,2,3-thiadiazol-5-ylurea) and NAA (α-naphthalene acetic acid) or TDZ and 2,4-D (2,4-dichlorophenoxyacetic acid). The frequency of explants with somatic embryo formation ranged from 70 to 95%. Embryos matured and germinated into plantlets.
The frequency of explants with embryo germination varied from 60 to 90%. On average, each explant was able to produce 60 plantlets.
Somatic embryos also occurred directly on the petiole explant surface of Syngonium cultured on MS medium supplemented with TDZ and NAA or TDZ and 2,4-D. The frequencies of petiole explants with somatic embryo occurrence and embryo germination were high, up to 85% and 80%, respectively.
Somatic embryos were able to germinate on the initial induction medium and after transferring onto MS medium containing BA (6-benzylaminopurine) and NAA. Fifty to 150 plantlets were regenerated from a single petiole explant. Lonicera was regenerated via indirect shoot organogenesis as calli formed from leaf explants cultured on B5 medium containing 2,4-D, BA, and NAA. Adventitious shoot occurred in calli cultured on B5 medium supplemented with BA, NAA, and kinetin.
Shoot rooted well on MS medium containing IBA. All regenerated plantlets grew vigorously after transplanting to a soilless container substrate in a shaded greenhouse.
These regeneration systems we have established will provide new means for propagation of pathogen-free, uniform plants and can potentially be scale-up for mass multiplication using bioreactors.
Authors
X. Wang, Y. Li, Q. Nie, J. Li, J. Chen, R.J. Henny
Keywords
Epipremnum, Lonicera, organogenesis, somatic embryogenesis, Syngonium
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