Articles
IN VITRO BULBLET REGENERATION AND EVALUATION OF FRITILLARIA ROYLEI HOOK. – A HIGH VALUE MEDICINAL HERB OF THE HIMALAYA
Article number
756_8
Pages
75 – 84
Language
English
Abstract
For the first time, culture conditions for in vitro bulblet regeneration of Fritillaria roylei Hook., a critically endangered medicinal plant of the Himalayas and one of the eight components of the vitality strengthening polyherbal ayurvedic formulation Astavarga, have been determined.
Sterilization of bulb scale explants was optimized and 83.3% establishment in tissue culture was achieved.
Two types of bulb scale explants (i.e., basal and distal) segments were tested for bulblet production and cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of kinetin (Kin), benzylaminopurine (BA) and α-naphthaleneacetic acid (NAA). As compared to distal segments, the basal segments of bulb scales showed better rate for bulblet regeneration percentage and plant regeneration.
Of the different concentrations and combinations of plant growth regulators tested, 5.0 µM kinetin with 2.0 µM NAA showed best results for percentage regeneration (95.8%) and number of bulblet produced per explant (10.1 ±0.63 bulblets) after eight weeks of culture.
Gas chromatographic profiles of diethyl ether extract of in vitro raised bulblets showed similar patterns to that of the naturally grown mother bulbs.
The results indicate that the in vitro bulblet production system after further optimization could provide promising options for harnessing the active constituents of the species and thereby helping in conserving the natural populations.
Sterilization of bulb scale explants was optimized and 83.3% establishment in tissue culture was achieved.
Two types of bulb scale explants (i.e., basal and distal) segments were tested for bulblet production and cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of kinetin (Kin), benzylaminopurine (BA) and α-naphthaleneacetic acid (NAA). As compared to distal segments, the basal segments of bulb scales showed better rate for bulblet regeneration percentage and plant regeneration.
Of the different concentrations and combinations of plant growth regulators tested, 5.0 µM kinetin with 2.0 µM NAA showed best results for percentage regeneration (95.8%) and number of bulblet produced per explant (10.1 ±0.63 bulblets) after eight weeks of culture.
Gas chromatographic profiles of diethyl ether extract of in vitro raised bulblets showed similar patterns to that of the naturally grown mother bulbs.
The results indicate that the in vitro bulblet production system after further optimization could provide promising options for harnessing the active constituents of the species and thereby helping in conserving the natural populations.
Authors
S.K. Joshi, U. Dhar, H.C. Andola
Keywords
liliaceae, bulbous plants, Astavarga, GC profiling, tissue culture
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