Articles
REGENERATION OF MULTIPLE SHOOTS FROM IMMATURE SEEDS AND MASS MULTIPLICATION OF DHATURA (DATURA METEL L.)
Article number
756_21
Pages
199 – 210
Language
English
Abstract
Dhatura (Datura metel L., Solanaceae) also called Thorn Apple or Indian Apple is widely found in India, Pakistan, Africa, Mediterranean region, Southern China, USA and Europe.
It is often included in the list for poisonous plants because it contains toxic tropane alkaloids, which are mainly responsible for its medicinal properties.
The dhatura plant contains such alkaloids hyoscyamine (atropine) and hyoscine generally in 2:1 ratio while the total alkaloid content ranges between 0.25% and 0.5%. Dhatura is an annual plant growing up to 4 ft tall, and is susceptible to frost since it is hardy to zone 9 only.
It flowers from June to July.
The plant prefers well-drained sandy loam soil.
It flourishes in the neutral and basic soils but can grow in very alkaline soil as well.
Increasing global demand for herbal medicines makes it inevitable to mass multiply this plant for the year round availability by utilizing biotechnological tools, which will not only ensure the mass production of clean plant material but will also help in the conservation of this species.
Therefore, we have started biotechnological investigations and have developed in vitro plant regeneration protocols utilizing fortification of media with different combinations of plant bioregulators.
It has been observed that the thin cell layer (TCL) culture medium supplemented with 2,4-D (2.25 mg/L) and BAP (0.25 mg/L) was found suitable to induce morphogenic callus in immature seed explants while the MS medium supplemented with BAP (1.0 mg/L) was found to be optimum for multiple shoot regeneration.
Liquid Lab Rocker Bioreactor system utilizing liquid MS medium supplemented with BAP produced significantly greater biomass of dhatura than did the magenta boxes containing the semi-solid MS medium supplemented with BAP.
It is often included in the list for poisonous plants because it contains toxic tropane alkaloids, which are mainly responsible for its medicinal properties.
The dhatura plant contains such alkaloids hyoscyamine (atropine) and hyoscine generally in 2:1 ratio while the total alkaloid content ranges between 0.25% and 0.5%. Dhatura is an annual plant growing up to 4 ft tall, and is susceptible to frost since it is hardy to zone 9 only.
It flowers from June to July.
The plant prefers well-drained sandy loam soil.
It flourishes in the neutral and basic soils but can grow in very alkaline soil as well.
Increasing global demand for herbal medicines makes it inevitable to mass multiply this plant for the year round availability by utilizing biotechnological tools, which will not only ensure the mass production of clean plant material but will also help in the conservation of this species.
Therefore, we have started biotechnological investigations and have developed in vitro plant regeneration protocols utilizing fortification of media with different combinations of plant bioregulators.
It has been observed that the thin cell layer (TCL) culture medium supplemented with 2,4-D (2.25 mg/L) and BAP (0.25 mg/L) was found suitable to induce morphogenic callus in immature seed explants while the MS medium supplemented with BAP (1.0 mg/L) was found to be optimum for multiple shoot regeneration.
Liquid Lab Rocker Bioreactor system utilizing liquid MS medium supplemented with BAP produced significantly greater biomass of dhatura than did the magenta boxes containing the semi-solid MS medium supplemented with BAP.
Authors
A. Yadav, N. Joshee, A.K. Yadav
Keywords
multiple shoot regeneration, liquid lab rocker bioreactor, biomass production
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