Articles
LIQUID CULTURE SYSTEM FOR SCUTELLARIA SPECIES
Article number
756_18
Pages
163 – 170
Language
English
Abstract
In vitro liquid culture of Scutellaria species (Scutellaria lateriflora, S. costaricana and S. baicalensis) was investigated in different conditions.
MS medium (Murashige and Skoog, 1962) (4.33 g L-1) supplemented with BA (5 μM), NAA (0.025 μM), sucrose (30 g L-1) was used for two experiments with addition of agar (5 g L-1) for agar treatments.
Agar, liquid stationary (LS), liquid agitated (LA) and liquid culture with floating paper (FP) were compared for fresh weight (FW), dry weight (DW), % dry weight (%DW), multiplication ratio (MR) along with sucrose, media and water use. S. lateriflora, S. costaricana, S. baicalensis were cultured for 4, 6, 8 weeks respectively.
LA and LS plantlets were more hyperhydric and had less MR compared to agar and FP plantlets.
Biomass was higher in liquid culture except S. baicalensis plantlets in LS. Since LA and LS gave higher biomass, our further research is focused on a polyester fiber matrix in liquid that may prevent hyperhydricity and provide greater biomass.
Agar, LS and liquid culture systems with matrix where plantlets kept in the gaseous phase were compared for similar responses with addition of hyperhydricity (visual assessment). The polyester fiber covered with seed germination paper was the matrix.
Agar, LS and fiber 30 had 30 ml initial media and, fiber 20 began with 20 ml and had additional 5 ml at week 4 and 6. Plantlets were harvested after 8 weeks.
Fiber 20 plantlets had significantly higher % DW for all species.
Agar, fiber 20 and fiber 30 plantlets were less hyperhydric and higher MR compared to LS plantlets.
Matrix prevented hyperhydricity and provided greater % DW with relatively higher MR.
MS medium (Murashige and Skoog, 1962) (4.33 g L-1) supplemented with BA (5 μM), NAA (0.025 μM), sucrose (30 g L-1) was used for two experiments with addition of agar (5 g L-1) for agar treatments.
Agar, liquid stationary (LS), liquid agitated (LA) and liquid culture with floating paper (FP) were compared for fresh weight (FW), dry weight (DW), % dry weight (%DW), multiplication ratio (MR) along with sucrose, media and water use. S. lateriflora, S. costaricana, S. baicalensis were cultured for 4, 6, 8 weeks respectively.
LA and LS plantlets were more hyperhydric and had less MR compared to agar and FP plantlets.
Biomass was higher in liquid culture except S. baicalensis plantlets in LS. Since LA and LS gave higher biomass, our further research is focused on a polyester fiber matrix in liquid that may prevent hyperhydricity and provide greater biomass.
Agar, LS and liquid culture systems with matrix where plantlets kept in the gaseous phase were compared for similar responses with addition of hyperhydricity (visual assessment). The polyester fiber covered with seed germination paper was the matrix.
Agar, LS and fiber 30 had 30 ml initial media and, fiber 20 began with 20 ml and had additional 5 ml at week 4 and 6. Plantlets were harvested after 8 weeks.
Fiber 20 plantlets had significantly higher % DW for all species.
Agar, fiber 20 and fiber 30 plantlets were less hyperhydric and higher MR compared to LS plantlets.
Matrix prevented hyperhydricity and provided greater % DW with relatively higher MR.
Authors
A. Tascan, J.W. Adelberg, N. Joshee , A.K. Yadav, M. Tascan
Keywords
Scutellaria, tissue culture, medicinal plant, polyester fiber, hyperhydricity
Online Articles (46)