Articles
APPLICATION OF DNA FINGERPRINTING TECHNOLOGY TO ESTIMATE GENETIC DIVERGENCE AMONG MANGO CULTIVARS-GENOTYPES
Article number
773_17
Pages
127 – 132
Language
English
Abstract
A study was designed to assess the genetic relatedness/diversity among selected mango cultivars/genotypes available in Pakistan.
Eleven indigenous and 4 exotic representative cultivars/genotypes were selected for a random amplified polymorphic DNA (RAPD) analysis.
Out of 25 random decamer primers surveyed, 20 amplified genomic DNA of all the genotypes.
Nineteen out of the 20 primers revealed polymorphism.
However, none of the primers produced a unique banding pattern for any genotype.
A total of 150 fragments, ranging from 240 to 2100 bp, were amplified.
A Euclidean distance matrix was calculated.
The coefficients were utilized to construct a dendrogram using the unweighted pair group of arithmetic means (UPGMA) algorithm.
The dendrogram shows the mango genotypes clustering into three group (A, B & C). The largest cluster A consists of commercial, indigenous cultivars while clusters B and C include a combination of exotics (Maya and Pope) as well as landraces.
Among the 15 accessions studied the most genetically diverse genotype is Kensington Pride (36.67%) followed by an indigenous landrace Yakta (35.33%). The results also confirmed the parentage of a promising new hybrid, Faiz Kareem.
The information generated with RAPD will be helpful in mango improvement as well as in the application of breeder rights in the country.
Eleven indigenous and 4 exotic representative cultivars/genotypes were selected for a random amplified polymorphic DNA (RAPD) analysis.
Out of 25 random decamer primers surveyed, 20 amplified genomic DNA of all the genotypes.
Nineteen out of the 20 primers revealed polymorphism.
However, none of the primers produced a unique banding pattern for any genotype.
A total of 150 fragments, ranging from 240 to 2100 bp, were amplified.
A Euclidean distance matrix was calculated.
The coefficients were utilized to construct a dendrogram using the unweighted pair group of arithmetic means (UPGMA) algorithm.
The dendrogram shows the mango genotypes clustering into three group (A, B & C). The largest cluster A consists of commercial, indigenous cultivars while clusters B and C include a combination of exotics (Maya and Pope) as well as landraces.
Among the 15 accessions studied the most genetically diverse genotype is Kensington Pride (36.67%) followed by an indigenous landrace Yakta (35.33%). The results also confirmed the parentage of a promising new hybrid, Faiz Kareem.
The information generated with RAPD will be helpful in mango improvement as well as in the application of breeder rights in the country.
Authors
I. Ahmad, A.U. Malik, S.A. Malik, N. Tabassam, M. Rahman, Y. Zafar
Keywords
Mangifera indica, genetic diversity, RAPD, parentage, Pakistan
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