PLANT REGENERATION VIA EMBRYOGENESIS AFTER GAMMA RADIATION IN BANANA (MUSA AAA)

The embryogenic cell suspension (ECS) of ‘Beida Aijiao’ (Musa AAA) and growing cell mass from ECS cultured on embryo regeneration medium for 11 days were selected for plant regeneration via embryogenesis after gamma radiation treatment at different dosages.
The results showed that the percentage of settled cell volume (SCV) increase in the radiated ECS was higher than that in the control; weight increase of regenerable material during the process of plant regeneration was negatively related to radiation dosages; the plant regeneration capacity also decreased with the increase in radiation dosage.
At the dose of 60 Gy, ECS died on embryo regeneration medium and no plants regenerated.
But at this dosage, plants could be obtained from the growing cell mass from the ECS cultured on embryo regeneration medium for 11 days.
Furthermore, the plant regeneration capacity of growing cell mass was much higher than that of ECS directly radiated with γ-ray at 4 radiation dosages, suggesting that culture of ECS on embryo regeneration medium could reduce the sensitivity of ECS to gamma radiation.
The optimal irradiation dosage was between 20 and 30 Gy for directly radiated ECS and between 30 and 40 Gy for growing cell mass from ECS cultured on RD1 embryo regeneration medium for 11 days, respectively.

XXVII International Horticultural Congress – IHC2006: International Symposium on Citrus and Other Tropical and Subtropical Fruit Crops

C.X. Xu, H.B. Chen, J. Luo

embryogenic cell suspension (ECS), irradiation dosages, Musa spp., plant regeneration capacity

https://doi.org/10.17660/ActaHortic.2008.773.18