Articles
AGROBACTERIUM-MEDIATED TRANSFORMATION OF CAROTENOID SYNTHESIS GENE IN KIWIFRUIT (ACTINIDIA DELICIOSA ‘HAYWARD’)
Article number
773_19
Pages
141 – 145
Language
English
Abstract
To improve the quality of kiwifruit (Actinidia deliciosa Hayward), the research team transformed with Agrobacterium tumefaciens EHA105 harboring the binary vector pCAMBIA 1300 associated with carotenoid synthesis gene such as geranylgeranyl diphosphate synthase (GGPS), phytoene desaturase (PDS), and ζ-carotene desaturase (ZDS), respectively.
Also, the binary vector had a hygromycin resistance gene (hptII) as a selectable marker.
Plant materials were prepared by micro-cross sectioning system of stems (sectioning of 800 µm). The transformed plant was selected on 1/2 MS medium containing 4.5×10-3 μM of 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.6×10-1 μM of zeatin, 5 μg ml-1 hygormycin, and 500 μg ml-1 cefotaxime.
Shoot regeneration efficiency of treated explants were 13.3% GGPS, 4.3% PDS, and 16.4% ZDS. Also, the confirmation of transformants was analyzed by PCR. The transformation efficiencies were 8% GGPS, 2.9% PDS, and 12.3% ZDS, respectively.
Also, the binary vector had a hygromycin resistance gene (hptII) as a selectable marker.
Plant materials were prepared by micro-cross sectioning system of stems (sectioning of 800 µm). The transformed plant was selected on 1/2 MS medium containing 4.5×10-3 μM of 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.6×10-1 μM of zeatin, 5 μg ml-1 hygormycin, and 500 μg ml-1 cefotaxime.
Shoot regeneration efficiency of treated explants were 13.3% GGPS, 4.3% PDS, and 16.4% ZDS. Also, the confirmation of transformants was analyzed by PCR. The transformation efficiencies were 8% GGPS, 2.9% PDS, and 12.3% ZDS, respectively.
Authors
Misum Kim, Seong-Cheol Kim, Ki-Chang Jang, Eun-Young Song, Sang-Jo Kang, Kwan-Jeong Song
Keywords
geranylgeranyl diphosphate synthase, phytoene desaturase, micro-cross sections, ζ-carotene desaturase
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