Articles
OBSERVATION OF ARBUSCULAR MYCORRHIZAL NETWORK SYSTEM BETWEEN TRIFOLIATE ORANGE AND SOME GRASSES UNDER WATERLOGGED CONDITIONS
Article number
773_9
Pages
69 – 75
Language
English
Abstract
An experiment was carried out to observe the distribution of arbuscular mycorrhizal fungal (AMF) hyphae both in the rhizospheres of trifoliate orange (Poncirus trifoliata) and in other plants under waterlogged conditions.
In this experiment, special acrylic boxes with three compartments, separated by nylon mesh screens, were used, where one outer compartment contained a seedling of trifoliate orange and the other one a seedling of trifoliate orange, Vulpia myuros or bahiagrass (Paspalum notatum). The center compartment was inoculated with Gigaspora margarit. The density of hyphae and the root colonization of trifoliate orange under waterlogged conditions increased when V. myuros or bahiagrass was intercropped.
Although waterlogging treatment decreased succinate dehydrogenase activity in trifoliate orange roots, decreased activity was alleviated by both intercropping of V. myuros or bahiagrass and inoculation of G. margarita. The other experiment was conducted to observe the internal hyphae of G. margarita in bahiagrass roots grown in water by using a light microscope and a scanning electron microscope (SEM). The hyphae were observed not only on the surface and in the epidermal cells of the roots, but also in the aerenchyma of the roots.
This observation suggests that AMF may get oxygen from the aerenchyma of bahiagrass roots.
In this experiment, special acrylic boxes with three compartments, separated by nylon mesh screens, were used, where one outer compartment contained a seedling of trifoliate orange and the other one a seedling of trifoliate orange, Vulpia myuros or bahiagrass (Paspalum notatum). The center compartment was inoculated with Gigaspora margarit. The density of hyphae and the root colonization of trifoliate orange under waterlogged conditions increased when V. myuros or bahiagrass was intercropped.
Although waterlogging treatment decreased succinate dehydrogenase activity in trifoliate orange roots, decreased activity was alleviated by both intercropping of V. myuros or bahiagrass and inoculation of G. margarita. The other experiment was conducted to observe the internal hyphae of G. margarita in bahiagrass roots grown in water by using a light microscope and a scanning electron microscope (SEM). The hyphae were observed not only on the surface and in the epidermal cells of the roots, but also in the aerenchyma of the roots.
This observation suggests that AMF may get oxygen from the aerenchyma of bahiagrass roots.
Authors
A. Matsumura, S. Horii, T. Ishii
Keywords
arbuscular mycorrhizal fungi, bahiagrass, Vulpia myuros, waterlogging
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