Articles
CHARACTERIZATION OF APHID-TRANSMITTED VIRUS ASSOCIATED WITH BLACK RASPBERRY DECLINE IN OREGON
Article number
777_49
Pages
327 – 332
Language
English
Abstract
A serious disease of black raspberry (Rubus occidentalis) was observed in Oregon in the last decade.
Plants showing mosaic symptoms declined rapidly and in many cases died over a period of several years.
Double-stranded RNA extraction from symptomatic black raspberry revealed the presence of two high molecular weight bands, which were cloned and partially sequenced.
Sequence analysis disclosed the presence of Black raspberry virus (BRV). A newly planted field of black raspberries in Oregon was studied to assess the rate of spread of BRV. The timing of BRV infection as it relates to aphid populations and flights was also determined.
Testing of nearby vegetation identified several symptomless Rubus hosts of BRV. It was determined that BRV spreads rapidly with a low aphid threshold and was associated with symptoms of decline in black raspberries in Oregon.
Plants showing mosaic symptoms declined rapidly and in many cases died over a period of several years.
Double-stranded RNA extraction from symptomatic black raspberry revealed the presence of two high molecular weight bands, which were cloned and partially sequenced.
Sequence analysis disclosed the presence of Black raspberry virus (BRV). A newly planted field of black raspberries in Oregon was studied to assess the rate of spread of BRV. The timing of BRV infection as it relates to aphid populations and flights was also determined.
Testing of nearby vegetation identified several symptomless Rubus hosts of BRV. It was determined that BRV spreads rapidly with a low aphid threshold and was associated with symptoms of decline in black raspberries in Oregon.
Publication
Authors
A. Halgren, I.E. Tzanetakis, R.R. Martin
Keywords
Rubus, Sadwavirus, dsRNA, RT-PCR
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