Articles
QUANTITATIVE ANALYSIS OF CURCUMINOIDS IN CURCUMA ZEDOARIA RHIZOMES IN THAILAND BY HPLC METHOD
Article number
786_18
Pages
169 – 174
Language
English
Abstract
A high performance liquid chromatographic (HPLC) method was developed and validated for quantitative determination of curcuminoid (curcumin, demethoxy-curcumin and bisdemethoxycurcumin) contents in extracts of the rhizomes of Curcuma zedoaria collected from ten locations in Thailand.
The analysis was carried out using a BDS Hypersil C18 column as a stationary phase, 0.1% glacial acetic acid aqueous solution and acetonitrile as mobile phases.
The detection was done at 425 nm.
The validation using curcumin, demethoxycurcumin and bisdemethoxy-curcumin as standards demonstrated good linearity, precision and accuracy at the concentration range of 2-6 mg/ml.
The content of curcumin in all powdered samples was in the range of 0.50±0.06 to 0.73±0.03% w/w (average 0.65±0.07% w/w) while the contents of demethoxycurcumin and bisdemethoxycurcumin were in the range of 0.23±0.02 to 1.43±0.55% w/w (average 0.91±0.35% w/w) and 0.12±0.01 to 0.44±0.21% w/w (average 0.25±0.10% w/w), respectively.
The highest average total curcuminoid content in the powdered samples was found to be 2.50±0.52% w/w while the lowest content was 1.06±0.31% w/w.
This information will be useful as guidance for further standardization of C. zedoaria raw material of which the content has not been reported before.
The analysis was carried out using a BDS Hypersil C18 column as a stationary phase, 0.1% glacial acetic acid aqueous solution and acetonitrile as mobile phases.
The detection was done at 425 nm.
The validation using curcumin, demethoxycurcumin and bisdemethoxy-curcumin as standards demonstrated good linearity, precision and accuracy at the concentration range of 2-6 mg/ml.
The content of curcumin in all powdered samples was in the range of 0.50±0.06 to 0.73±0.03% w/w (average 0.65±0.07% w/w) while the contents of demethoxycurcumin and bisdemethoxycurcumin were in the range of 0.23±0.02 to 1.43±0.55% w/w (average 0.91±0.35% w/w) and 0.12±0.01 to 0.44±0.21% w/w (average 0.25±0.10% w/w), respectively.
The highest average total curcuminoid content in the powdered samples was found to be 2.50±0.52% w/w while the lowest content was 1.06±0.31% w/w.
This information will be useful as guidance for further standardization of C. zedoaria raw material of which the content has not been reported before.
Authors
S. Paramapojn, W. Gritsanapan
Keywords
curcumin, demethoxycurcumin, bisdemethoxycurcumin, steam distillation, liquid chromatography
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