A STANDARD SET OF ACCESSIONS, MICROSATELLITES AND GENOTYPES FOR HARMONISING THE FINGERPRINTING OF CHERRY COLLECTIONS FOR THE ECPGR

Increasingly, microsatellites (SSRs) are being used to fingerprint accessions of tree-fruit crops including cherry.
However, opportunities to compare results between collections are hampered, as different laboratories tend to use different protocols.
The ECPGR (European Collaborative Programme for Genetic Resources) has a working group for Prunus, which aims to facilitate the conservation of genetic resources in European collections.
A recent workshop addressed the questions of harmonising datasets so that SSR fingerprints can be used more effectively to characterise accessions in the various ECPGR collections.
In the case of cherry, eight reference accessions – five of P. avium and three from related species – were chosen; and 16 unlinked SSR primer pairs, mined from several Prunus species, were identified that give good coverage of the cherry genome, are polymorphic in sweet cherry, and amplify single loci.
The reference set of genotypes is presented so that future fingerprints can be harmonised to these values.
Four of the SSRs (CPPCT006 and 022 and EMPA002 and 017 mapping to G8, G7, G1, and G2 respectively) have been provisionally designated ‘universal’ primers as they are transferable across a wide range of Prunus.

XII EUCARPIA Symposium on Fruit Breeding and Genetics

J.B. Clarke, K.R. Tobutt

Prunus avium, microsatellite, standard, transferability

https://doi.org/10.17660/ActaHortic.2009.814.104