EVALUATION OF APPLE PLANTS FOR WINTER RESISTANCE BY PEROXIDASE ISOENZYME ANALYSIS

The research objects were 20 apple cultivars and hybrids from various classes of winter resistance, which had been tested using the field method, and 29 cultivars that had not been studied before.
The modelling of damaging factors was performed using a deep freezer and refrigerator.
Leaves, buds, and bark without treatment served as controls.
The electrophoresis method of PAGE in native conditions and spectrophotometric methods of biochemical indices detection were involved in the study.
A significant difference was observed in leaf peroxidaze isoenzyme composition for different winter resistance levels at the freezing condition “-17°C during 24 hours”. The isoperoxidases with Rf~0.28 and 0.30 are the markers of apple cultivars with low winter resistance, whereas cultivars with high winter resistance are characterized by specific fast-moving components with Rf~0.53, 0.56, and 0.66. The assay carried out in 2005 showed that cultivars ‘Arlet’, ‘Pinova’, ‘Аlkmene’, and ‘Redcroft’ had an isoform with Rf 0.30, which vanished from the peroxidaze izoenzyme composition in leaves, whereas ‘Empire’ and ‘Reanda’ had an isoform with Rf 0.28, disappearing after freezing.
Further research in 2006 revealed that freezing of ‘Pinova’, ‘Redcroft’, and ‘Alkmene’ leaves caused the disappearance of a slow-moving isoform with Rf~0.28.

XII EUCARPIA Symposium on Fruit Breeding and Genetics

Z.A. Kazlouskaya, E.N. Biruk

apple, winter resistance, electrophoresis, peroxidaze, markers, Belarus

https://doi.org/10.17660/ActaHortic.2009.814.138