IDENTIFICATION OF S-ALLELES IN 25 SWEET CHERRY (PRUNUS AVIUM L.) CULTIVARS BY ALLELE-SPECIFIC PCR AMPLIFICATION

Self-incompatibility and cross incompatibility between various pairs of sweet cherry cultivars is attributed to the multi-allelic locus S, expressed gametophytically.
In this study the S-genotypes of 25 Iranian and foreign sweet cherry cultivars were studied using the Polymerase Chain Reaction (PCR) method.
Thirteen specific primers designed for amplification of S₁, S₂, S₃, S₄, S₅, S₆, S₇, S₉, S₁₀, S₁₂, S₁₃, S₁₄, and S₁₆ alleles were employed.
Thirty-five out of the 50 possible S-alleles were detected using specific primers.
Two S-alleles were amplified in 13 cultivars and for nine cultivars just one S-allele could be amplified.
No S-allele could be detected in two cultivars.
Only S₃, S₄, and S₁₄ alleles were detected in Iranian sweet cherry cultivars.
These three S-alleles were very frequent and observed in 59, 63, and 13% of the cultivars studied respectively.
S3S4 was the most frequent cross incompatibility group (CIG) observed in 36% of Iranian sweet cherry cultivars.
Moreover, one new CIG was proposed with S₄S₁₄ genotype comprising ‘Sorati hamadan’, ‘Binam’, and ‘Siah gazvin’ cultivars.
Although allele-specific PCR amplification was not capable enough to identify all the S-alleles in the cultivars studied, it provides a rapid and efficient approach for identifying the S-genotypes of sweet cherry cultivars.

XII EUCARPIA Symposium on Fruit Breeding and Genetics

A. Ershadi, E.H. Moghadam

sweet cherry, self-incompatibility, cross-incompatibility, polymerase chain reaction, S-genotype

https://doi.org/10.17660/ActaHortic.2009.814.105