Home > XII EUCARPIA Symposium on Fruit Breeding and Genetics > PRODUCTION OF TRANSGENIC PEAR PLANTS EXPRESSING FERRITIN GENE WITH THE AIM TO REDUCE FIRE BLIGHT SUSCEPTIBILITY

Articles

PRODUCTION OF TRANSGENIC PEAR PLANTS EXPRESSING FERRITIN GENE WITH THE AIM TO REDUCE FIRE BLIGHT SUSCEPTIBILITY

Article number

814_132

Pages

781 – 786

Language

English

Abstract

Fire blight caused by the bacteria Erwinia amylovora is one of the most important diseases of pear.
This causal agent produces a siderophore (desferrioxamine), which was identified as one of its virulence factors.
This protein enables the bacteria to overcome conditions of iron limitation encountered in host tissues, and may also protect the bacteria against active oxygen species.
Previous experiments indicate that the use of an iron chelator protein, encoded by the bovine lactoferrin gene, reduces fire blight symptoms in some transgenic pear clones.
The aim of the present work is to test the ability of a more efficient iron chelator, plant ferritin, to reduce fire blight susceptibility in pear.
In the literature, ferritin genes have been overexpressed under the control of a constitutive promoter in different transgenic plant species for various purposes.
In several cases, the constitutive expression of ferritin produced negative effects such as reduced growth and chlorophyll content.
Therefore, we decided to place the exogenous ferritin gene from pea under the control of a pathogen inducible promoter (sgd24) in comparison with a constitutive promoter (CaMV 35S). Two pear varieties, Conférence (CF) and Passe-Crassane (PC) were transformed using both constructs.
Transformation rates depended on variety and construct.
They were respectively of 12 and 4.3% for CF and PC using the sgd24-ferritin construct.
Only PC was transformed with the 35S-ferritin construct, with a transformation rate of 2%. First analyses of the transgenic clones by RT-PCR showed the expression of pea ferritin in both constructs and in all clones.
The transgenic clones were acclimatized in greenhouse and exhibited normal growth.
Quantification of ferritin gene expression, ferritin protein accumulation, and evaluation of fire blight resistance are underway.

Publication

XII EUCARPIA Symposium on Fruit Breeding and Genetics

Authors

S. Djennane, C. Cesbron, S. Sourice, K. Loridon, E. Chevreau

Keywords

Erwinia amylovora, iron, gene transfer, Pyrus communis

Full text

https://doi.org/10.17660/ActaHortic.2009.814.132

Online Articles (147)

1. FRUIT BREEDING IN SPAIN

G. Llácer

2. PROGRESS IN EVALUATING MALUS SIEVERSII FOR DISEASE RESISTANCE AND HORTICULTURAL TRAITS

3. ESTABLISHMENT OF A CORE COLLECTION TO OPTIMISE THE CONSERVATION OF CHERIMOYA (ANNONA CHERIMOLA MILL.) GENETIC RESOURCES USING SSR INFORMATION

P. Escribano | M.A. Viruel | J.I. Hormaza

4. APPLE GENE BANKS – FOR BREEDING, RESEARCH OR PUBLIC ENTERTAINMENT?

H. Nybom | L. Garkava-Gustavsson

5. USING CENTRAL ASIAN GERMPLASM TO IMPROVE FRUIT QUALITY AND ENHANCE DIVERSITY IN CALIFORNIA ADAPTED APRICOTS

C.A. Ledbetter

6. MOROCCAN FIG GENETIC STRUCTURE SUGGESTING A LOCAL SELECTION

7. PEACH GERMPLASM FROM THE EBRO MIDDLE VALLEY IN THE CITA PEACH BREEDING PROGRAMME

J.M. Alonso | M.T. Espiau | J.M. Ansón | M. Carrera

8. EVALUATION OF LOCAL PLUM VARIETIES (PRUNUS DOMESTICA L.) FROM THE ROMANIAN NATIONAL COLLECTION

M. Butac | S. Budan

9. GENETIC DIVERSITY ANALYSIS AND MOLECULAR CHARACTERIZATION OF TUNISIAN FIGS (FICUS CARICA L.)

10. HARMONISING FINGERPRINTING PROTOCOLS TO ALLOW COMPARISONS BETWEEN GERMPLASM COLLECTIONS – PYRUS

K.M. Evans | F. Fernández-Fernández | C. Govan

11. ASSESSMENT OF RELATIONSHIPS IN TUNISIAN POMEGRANATE (PUNICA GRANATUM L.) CULTIVARS BY POMOLOGICAL AND AFLP MARKERS

R. Jbir | N. Hasnaoui | M. Marrakchi | M. Trifi | M. Mars

12. EVALUATION OF THE CURRENT APRICOT VARIABILITY IN TUNISIA – COMPARISON WITH PREVIOUSLY DESCRIBED CULTIVARS

L. Krichen | N. Trifi-Farah | M. Marrakchi | J.M. Audergon

13. SELECTION AND UTILIZATION OF TABLE CORNELIAN CHERRY (CORNUS MAS L.)

14. COMPARATIVE ANALYSIS OF GENETIC DIVERSITY IN TUNISIAN DATE-PALM (PHOENIX DACTYLIFERA L.) AS REVEALED BY RAPDS AND AFLPS

15. ESTIMATING USEFUL DIVERSITY IN THE NORWEGIAN CORE COLLECTION OF APPLES

D. Røen | A. Ekholm | K. Rumpunen

16. MOLECULAR CHARACTERIZATION OF IRANIAN ALMOND CULTIVARS AND RELATED WILD SPECIES USING AMPLIFIED FRAGMENT-LENGTH POLYMORPHISMS (AFLPS)

B. Shiran | K. Sorkheh | V. Rouhi | T.M. Gradziel | P. Martínez-Gómez

17. CHARACTERIZATION OF FRUIT GENETIC RESOURCES IN SWITZERLAND

D. Szalatnay | R. Eder-Bauermeister | B. Duffy | M. Kellerhals

18. COMPARATIVE ANALYSIS OF GENETIC DIVERSITY IN TUNISIAN COLLECTIONS OF DATE-PALM CULTIVARS BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA AND INTER SIMPLE SEQUENCE REPEATS FINGERPRINTS

19. EVALUATION OF SOME NATIVE SWEET CHERRY GENOTYPES COLLECTED EX SITU INTO ROMANIAN NATIONAL GERMPLASM

S. Budan | L. Petre | G. Gradinariu

20. “FAST-BREEDING” IN APPLE: A STRATEGY TO ACCELERATE INTROGRESSION OF NEW TRAITS INTO ELITE GERMPLASM

R.K. Volz | E. Rikkerink | P. Austin | T. Lawrence | V.G.M. Bus

21. TOWARDS A STRATEGY FOR PHENOTYPING ARCHITECTURAL TRAITS IN MATURE F₁ HYBRIDS OF AN APPLE PROGENY

V. Segura | J.J. Kelner | P.E. Lauri | E. Costes

22. SELECTION EFFICIENCY IN APPLE BREEDING

M. Kellerhals | M. Spuhler | B. Duffy | A. Patocchi | J.E. Frey

23. APRICOT AND PEACH BREEDING PROGRAMS FROM THE IVIA

J. Martínez-Calvo | A. Font | G. Llácer | M.L. Badenes

24. PENTA AND TARDONA: TWO NEW EXTRA-LATE FLOWERING SELF-COMPATIBLE ALMOND CULTIVARS

25. NEW POLISH WINTER CULTIVARS OF APPLE

A.A. Przybyla | M. Kantorowicz-Bąk | K. Tomala

26. CISGENESIS IS A PROMISING APPROACH FOR FAST, ACCEPTABLE AND SAFE BREEDING OF PIP FRUIT

27. ADVANCES IN FRUIT BREEDING IN EASTERN CANADA – ROLE OF PHYTOCHEMICALS IN DESIGNING SPECIALTY FRUITS

28. THE IRTA-HORTRESEARCH APPLE SCION BREEDING PROGRAMME: AIMING FOR HIGH FRUIT QUALITY UNDER WARM GROWING CONDITIONS

29. CHILL AND HEAT REQUIREMENTS FOR BLOOMING OF THE CITA ALMOND CULTIVARS

J.M. Alonso | R. Socias i Company

30. NEW RECENT SELECTIONS ISSUED FROM INRA’S APRICOT BREEDING PROGRAM

31. SOME RESULTS OF STUDY CONCERNING IN VITRO TECHNIQUES OF BREEDING AND IMPROVEMENT OF PRUNUS DOMESTICA L.

32. THE SLOVAK APRICOT BREEDING PROGRAM

D. Benedikova

33. FOUR NEW SUMMER APPLES FROM THE CZECH REPUBLIC

J. Blaek | F. Paprtein

34. INHERITANCE OF RED FRUIT SKIN AND FLESH COLOR IN PYRUS COMMUNIS CULTIVARS

N. Braniste | S. Budan

35. FRUIT BEARING HABIT OF NINE SWEET CHERRY CULTIVARS

V. Dzhuvinov | K. Kolev

36. PREVIEW OF THE SECOND CLONAL SELECTION FROM THE AUTOCHTHONOUS PEACH POPULATION “AMARILLOS TARDÍOS DE CALANDA” (LATE YELLOW PEACHES OF CALANDA)

J.L. Espada | J. Romero | R. Socias i Company | J.M. Alonso

37. MULTIDISCIPLINARY CHARACTERIZATION OF PRIMOCANE RASPBERRIES COMPARED TO FLORICANE FRUITING CULTIVARS

38. YIELD COMPONENT OF CULTIVAR DIFFERENCES IN STRAWBERRY

F. Grigorova | V. Dzhuvinov

39. PRELIMINARY RESULTS TO ESTABLISH THE DAAO SYSTEM AS AN ALTERNATIVE SELECTION STRATEGY ON APPLE

C. Hättasch | M.-V. Hanke | H. Flachowsky

40. EDEN^TM, A NON-BROWNING APPLE SUITABLE FOR FRESH CUT SLICE AND FRUIT SALAD

41. ‘ST-JEAN D’ORLÉANS’ STRAWBERRY

42. TOWARDS NEW CULTIVARS OF STEVNSBÆR SOUR CHERRIES IN DENMARK

K. Kristiansen | M. Jensen

43. STABILITY OF MAIN APPLE CULTIVARS GROWN IN TRANSYLVANIA DETERMINED BY MEANS OF NONPARAMETRIC ANALYSES

44. STUDIES ON THE SOMACLONAL VARIATION OF THE PERSIMMON (DIOSPYROS KAKI THUNB.) CV. ‘ROJO BRILLANTE’ AS A BREEDING TOOL

M.M. Naval | G. Llácer | M.L. Badenes | E. Giordani

45. GENETIC IMPROVEMENT OF APPLES AT THE FACULTY OF AGRICULTURE – NOVI SAD

V. Ognjanov | M. Ljubojević | B. Vračević

46. JOINT EXPERIMENTS IN FRANCE AND CATALONIA OF NEW FLAT PEACHES-NECTARINES AND CANNING PEACHES FROM INRA

T. Pascal | I. Iglesias Castellarnau | P. Blanc | C. Pitiot

47. ‘XENIA’, A NEW PEAR CULTIVAR FROM MOLDOVA, FIRST RESULTS IN THE NETHERLANDS

G. Heijerman-Peppelman | V. Bucarciuc | H. Kemp | O. Pasat

48. THE LAST TWENTY YEARS OF BLACKCURRANT (RIBES NIGRUM L.) BREEDING WORK IN POLAND

S. Pluta | E. Żurawicz

49. NEW POLISH VEGETATIVE MUTANT ROOTSTOCKS FOR APPLE

A.A. Przybyla | P. Bielicki | A. Czynczyk

50. VERIFYING THE PARENTS OF THE PILLNITZER APPLE CULTIVARS

S. Reim | H. Flachowsky | M.-V. Hanke | A. Peil

51. SOUR CHERRIES PRUNUS CERASUS L. WITH COLUMNAR TREE HABIT

M. Schuster

52. G-2-25, A NEW SELF-COMPATIBLE AND LATE-BLOOMING ALMOND SELECTION

R. Socias i Company | O. Kodad | J.M. Alonso | A.J. Felipe

53. STANDARD TESTING AGREEMENT FOR FRUIT PLANT MATERIAL DEVELOPED BY EUFRIN WORKING GROUP

R. Stehr

54. BREEDING FOR RED FLESH COLOUR IN APPLE: PROGRESS AND CHALLENGES

55. GROWTH CHARACTERISTICS OF THE SMALL-FRUIT MUTANT IN JAPANESE PERSIMMON (DIOSPYROS KAKI THUNB.)

H. Yamane | M. Ichiki | R. Tao

56. ‘HANHONG’ PEAR

57. RESULTS FROM THE PEACH BREEDING PROGRAMME IN BULGARIA

A.T. Zhivondov | V.B. Bozhkova

58. ‘KOSSARA’ – NEW VERY EARLY SWEET CHERRY

A. Zhivondov | P. Gercheva

59. NEW PEAR CULTIVARS FROM THE CZECH REPUBLIC

F. Paprtein | J. Blaek | J. Bouma

60. QTL ANALYSIS FOR LEAF GAS EXCHANGE IN AN APPLE PROGENY GROWN UNDER ATMOSPHERIC CONSTRAINTS

J.L. Regnard | V. Segura | N. Merveille | C.E. Durel | E. Costes

61. SELF-(IN)COMPATIBILITY IN JAPANESE APRICOT (PRUNUS MUME)

R. Tao | T. Habu | K. Fukuta | D. Matsumoto | H. Yamane

62. INFLUENCE OF JUVENILITY AND OTHER FACTORS IN THE EVALUATION OF FLOWERING TIME IN YOUNG APRICOT SEEDLINGS

J.A. Campoy | D. Ruiz | J. Egea | P. Martínez-Gómez

63. REPRODUCTIVE BIOLOGY OF AVOCADO (PERSEA AMERICANA MILL.) IN SOUTHERN SPAIN

M.L. Alcaraz | J.I. Hormaza

64. IDENTIFICATION OF S-ALLELES IN SEVERAL APPLE CULTIVARS

K. Bokszczanin | A. Palucha | A.A. Przybyla

65. BULKED SEGREGANT ANALYSIS FOR THE IDENTIFICATION OF MOLECULAR MARKERS LINKED TO SELF-COMPATIBILITY IN ‘CRISTOBALINA’ SWEET CHERRY

A.M. Cachi | A. Wünsch

66. EFFECT OF VARIOUS ROOTSTOCKS AND TREE SPACING ON THE SIZE OF ‘VANDA’ SWEET CHERRY FRUITS

Z.S. Grzyb | E. Rozpara

67. S-ALLELE IDENTIFICATION IN JAPANESE PLUM CULTIVARS BY PCR AND CROSS-POLLINATION

M.E. Guerra | J. Rodrigo | M. López-Corrales | A. Wünsch

68. STUDIES ON MRNA EXPRESSION OF GENES INVOLVED IN FLORAL MERISTEM TRANSITION OF APPLE (MALUS DOMESTICA BORKH)

C. Hättasch | D. Kapturska | H. Flachowsky | F. Dunemann | M.-V. Hanke

69. POLLEN DEVELOPMENT AND CHILLING REQUIREMENTS IN APRICOT CULTIVARS

C. Julian | M. Herrero | J. Rodrigo

70. REVIEW AND UPDATE OF SELF-INCOMPATIBILITY ALLELES IN ALMOND

O. Kodad | R. Socias i Companya

71. PERFORMANCE OF ‘GLOSTER’ AND ‘MELROSE’ APPLE TREES ON CZECH ROOTSTOCKS AND M 9 CLONES

J. Kosina

72. THE INFLUENCE OF GENOTYPE ON GERMINATION OF APPLE SEEDS

M. Lewandowski | E. Żurawicz

73. OUTCROSSING RATE AND STIGMATIC RECEPTIVITY IN CHERIMOYA (ANNONA CHERIMOLA MILL., ANNONACEAE), A DICHOGAMOUS SUBTROPICAL FRUIT TREE SPECIES

J. Lora | J.I. Hormaza | M. Herrero

74. STATISTICAL ASSESSMENT OF THE COMPONENTS OF FRUIT YIELD IN BLACKCURRANT (RIBES NIGRUM L.)

W. Mądry | S. Pluta | E. Żurawicz

75. DETERMINATION OF CROSS-COMPATIBILITY OF NEW ‘MARCONA-TYPE’ AND ‘DESMAYO LARGUETA-TYPE’ ALMOND ACCESSIONS FROM ITAP

P.J. Martínez-García | F. Mañas | F. Dicenta | E. Ortega

76. MORPHOLOGICAL AND HISTOLOGICAL CHARACTERISTICS RELATED WITH PHASE CHANGE (JUVENILE/ADULT) IN OLIVE LEAVES AND ITS DETERMINATION BY NEAR INFRARED REFLECTANCE SPECTROSCOPY

I. Moreno-Alías | A. Gracia | L. León | R. De la Rosa | H.F. Rapoport

77. MORPHOLOGICAL AND HISTOCHEMICAL FEATURES OF COMPATIBLE AND INCOMPATIBLE STEM UNIONS

A. Pina | P. Errea

78. THE USE OF ROLLING CIRCLE AMPLIFICATION&NDASH;RACE (RCA-RACE) FOR THE ISOLATION OF FULL-LENGTH GENES FROM PEACH (PRUNUS PERSICA), INVOLVED IN FRUIT DEVELOPMENT AND THE STUDY OF THEIR ROLE IN PIT HARDENING AND SPLIT-PIT PROCESS

E. Tani | A.N. Polidoros | A.S. Tsaftaris

79. THE INS AND OUTS OF TOMATO FRUIT PEEL METABOLOME

80. FRUIT QUALITY IN ALMOND: PHYSICAL ASPECTS FOR BREEDING STRATEGIES

R. Socias i Company | J.M. Alonso | O. Kodad

81. CYANOGENIC GLUCOSIDE PATTERNS IN SWEET AND BITTER ALMONDS

R. Sánchez-Pérez | B. Lindberg Møller | C.E. Olsen | F. Dicenta

82. MICRO-METHODS FOR GENOTYPIC SCREENING OF HAZELNUT (CORYLUS AVELLANA L.) SEEDS TO ACCELERATE BREEDING FOR SEED METABOLITE IMPROVEMENT

L. Kuzmanović | M. Delfini | E. Rugini | P. Gutiérrez-Pesce | C. De Pace

83. FRUIT QUALITY ATTRIBUTES OF NEW PEACH AND NECTARINE VARIETIES UNDER SELECTION IN THE EBRO VALLEY CONDITIONS (SPAIN)

C.M. Cantin | J. Torrents | Y. Gogorcena | M.A. Moreno

84. RAPID AND NON-DESTRUCTIVE DETERMINATION OF SOLUBLE SOLIDS CONTENT AND TITRATABLE ACIDITY IN APRICOT USING NEAR-INFRARED SPECTROSCOPY (NIR)

85. TOWARD THE ISOLATION OF THE D GENE CONTROLLING THE ACIDITY OF PEACH FRUIT BY POSITIONAL CLONING

86. METABOLIC ENGINEERING OF FLAVONOID BIOSYNTHESIS IN APPLE (MALUS DOMESTICA BORKH.)

87. HETEROLOGOUS COMPARATIVE GENOMICS TO IDENTIFY CANDIDATE GENES IMPACTING FRUIT QUALITY IN APPLE (MALUS X DOMESTICA BORKH.)

88. ISAFRUIT – STUDY OF THE GENETIC BASIS OF PRUNUS FRUIT QUALITY IN TWO PEACH AND TWO APRICOT POPULATIONS

89. BROWNING POTENTIAL OF NEW APPLE VARIETIES

J. DeEll | P. Toivonen | S. Khanizadeh | C. Hampson

90. DETECTION OF QTLS CONTROLLING MAJOR FRUIT QUALITY COMPONENTS IN PEACH WITHIN THE EUROPEAN PROJECT ISAFRUIT

91. CHANGES OF PROFILES OF GENES INVOLVED IN THE SYNTHESIS OF PHENOLIC COMPOUNDS DURING GRAPEVINE FRUIT RIPENING: THE ROLE OF LIGHT

92. PHYTOCHEMICAL PROFILE OF SELECTED PROCESSING APPLE GENOTYPES

93. PROFILE OF ANTIOXIDANT ACTIVITIES OF SELECTED STRAWBERRY GENOTYPES

94. FATTY ACID AND TOCOPHEROL CONCENTRATIONS IN ALMOND OIL AND ITS IMPLICATION IN A BREEDING PROGRAMME

O. Kodad | R. Socias i Company

95. BREEDING POTENTIAL OF FRAGARIA VESCA F. ALBA IN RESPECT TO VOLATILE COMPOUNDS

96. GENE EXPRESSION PATTERNS UNDERLYING RED SKIN COLOUR IN ‘MAX RED BARTLETT’ (PYRUS COMMUNIS), A ‘WILLIAMS’ BUD MUTATION

L. Pierantoni | L. Dondini | S. Musacchi | B.J.S. Winkel | S. Sansavini

97. EVOLUTION OF APRICOT FRUIT QUALITY ATTRIBUTES IN THE NEW RELEASED CULTIVARS

98. PROTON TRANSFER REACTION-MASS SPECTROMETRY ANALYSIS IS A VALUABLE TOOL FOR THE IDENTIFICATION OF GENOMIC REGIONS RELATED TO VOLATILE ORGANIC COMPOUNDS

99. GENETIC LINKAGE MAP CONSTRUCTION AND IDENTIFICATION OF QTLS AFFECTING TIME OF INITIAL VEGETATIVE BUDBREAK IN APPLE (MALUS X DOMESTICA BORKH.)

M.M. van Dyk | I.F. Labuschagne | D.J.G. Rees

100. NUTRITIONAL ENHANCEMENT IN APPLE: IDENTIFICATION OF QTLS FOR FRUIT VITAMIN C CONTENT AND THEIR STABILITY OVER DIFFERENT PRODUCTION YEARS

M.W. Davey | J. Keulemans

101. REFERENCE GENETIC LINKAGE MAPS OF EUROPEAN AND JAPANESE PEARS

102. DEVELOPMENT OF A SET OF APPLE SSRS MARKERS SPANNING THE APPLE GENOME, GENOTYPING OF HIDRAS PLANT MATERIAL AND VALIDATION OF GENOTYPIC DATA

103. A GENETIC LINKAGE MAP OF OLEA EUROPAEA L. USING A PSEUDO-TEST CROSS- MAPPING STRATEGY BASED ON SSR, AFLP, ISSR, RAPD AND SCAR MARKERS

104. A STANDARD SET OF ACCESSIONS, MICROSATELLITES AND GENOTYPES FOR HARMONISING THE FINGERPRINTING OF CHERRY COLLECTIONS FOR THE ECPGR

J.B. Clarke | K.R. Tobutt

105. IDENTIFICATION OF S-ALLELES IN 25 SWEET CHERRY (PRUNUS AVIUM L.) CULTIVARS BY ALLELE-SPECIFIC PCR AMPLIFICATION

A. Ershadi | E.H. Moghadam

106. CLONING OF THE SELF-COMPATIBILITY S^F LOCUS IN ALMOND

107. GENETIC DIVERSITY OF MUME, APRICOT AND JAPANESE PLUM CULTIVARS ASSESSED BY SSR MARKERS

108. S-ALLELE GENOTYPING OF LOCAL SPANISH ALMOND CULTIVARS

O. Kodad | A. Sánchez | M. Oliveira | R. Socias i Company

109. A GENERALIZED PROCEDURE TO PREPARE PCR-GRADE DNA FROM DIFFERENT CORYLUS AVELLANA L. PLANT TISSUES FOR PARENTAGE ANALYSIS

L. Kuzmanović | E. Rugini | C. De Pace

110. ANALYSIS OF EXPRESSED SEQUENCE TAGS FROM JAPANESE PEAR ‘HOUSUI’

C. Nishitani | T. Shimizu | H. Fujii | S. Terakami | T. Yamamoto

111. NEW POLYMORPHIC EST-BASED MOLECULAR MARKERS IN THREE MALUS X DOMESTICA (BORKH.) CULTIVARS (FIESTA, PRIMA, DISCOVERY)

112. INTER-COMPATIBILITY RELATIONSHIPS IN OLIVE AS REVEALED BY PATERNITY TESTS WITH SSR MARKERS

E. Rodríguez-Castillo | A. Díaz | A. Belaj | R. De la Rosa

113. CHARACTERIZATION OF LENGTH POLYMORPHISMS AT THE 3’UTR OF THE MALOIDEAE S-RNASE AND ITS APPLICATION FOR S-GENOTYPING IN EUROPEAN PEAR

J. Sanzol

114. QTL ANALYSIS FOR GROWTH AND BRANCHING TRAITS ANNUALLY ASSESSED ALONG THE TRUNK OF THREE-YEAR-OLD APPLE HYBRIDS

V. Segura | C.E. Durel | E. Costes

115. APPLE FRUIT: TOWARDS THE IDENTIFICATION OF GENES SHOWING A MODULATED EXPRESSION DURING DEVELOPMENT

V. Soglio | H. Schouten | F. Costa | D. Perini | L. Gianfranceschi

116. BIN MAPPING OF EST-SSRS IN APPLE (MALUS X DOMESTICA BORKH.)

M.M. van Dyk | D.J.G. Rees

117. SSR MARKERS FOR FINGERPRINTING PRUNUS SPECIES

A. Wünsch

118. STUDY OF THE ORIGIN OF THE CULTIVATED ALMOND USING NUCLEAR AND CHLOROPLAST DNA MARKERS

119. RETROTRANSPOSON BASED MARKERS TO DISCRIMINATE SPORTS IN PEAR

S. Venturi | P. De Franceschi | L. Dondini | S. Sansavini

120. RESISTANCE TO ROOT KNOT NEMATODES IN PRUNUS: CHARACTERIZATION OF SOURCES, MARKER-ASSISTED SELECTION AND CLONING STRATEGY FOR THE MA GENE FROM MYROBALAN PLUM

D. Esmenjaud

121. BIOCHEMICAL AND MOLECULAR ASPECTS INVOLVED IN WATERLOGGING TOLERANCE IN PRUNUS ROOTSTOCKS

M.L. Amador | S. Sancho | M.J. Rubio-Cabetas

122. TOWARDS AN UNDERSTANDING OF THE INHERITANCE OF HYPERSENSITIVITY RESISTANCE AGAINST THE SHARKA VIRUS IN EUROPEAN PLUM (PRUNUS DOMESTICA L.): GENERATION OF INTERSPECIFIC HYBRIDS WITH LOWER PLOIDY LEVELS

M. Neumüller | S. Lanzl | W. Hartmann | W. Feucht | D. Treutter

123. MAPPING THE RESISTANCE TO PLUM POX VIRUS (PPV) CARRIED BY THE APRICOT ‘STARK EARLY ORANGE’ USING DIFFERENT ENVIRONMENTAL CONDITIONS AND GENETIC BACKGROUNDS; COMPARISON WITH P. DAVIDIANA

124. IDENTIFICATION OF CULTIVABLE BACTERIA FROM IN VITRO CULTURES OF APPLE

H. Flachowsky | M.-V. Hanke

125. A PROPOSAL FOR THE NOMENCLATURE OF VENTURIA INAEQUALIS RACES

126. IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF VF-LIKE CANDIDATE GENES IN CULTIVATED APPLES AND SELECTIONS FROM MALUS SIEVERSII

A. Boudichevskaia | H. Flachowsky | F. Dunemann

127. IDENTIFICATION AND VALIDATION OF QTLS LINKED TO FIRE BLIGHT RESISTANCE IN MALUS AND THEIR APPLICABILITY IN MARKER-ASSISTED SELECTION

128. SCREENING FOR BOTRYTIS CINEREA RESISTANT APPLE CULTIVARS

A. Auwerkerken | M.W. Davey | A. Van den Putte | J. Keulemans

129. CHANGES IN RESPONSE TO SCAB INFECTION (VENTURIA INAEQUALIS CKE. WINT.) ON APPLE CULTIVARS DURING THE LAST 30 YEARS IN THE CZECH REPUBLIC

J. Blaek | F. Paprtein | R. Vávra

130. PHENOTYPIC SELECTION ASSISTED BY MOLECULAR MARKERS FOR SCAB RESISTANCE IN APPLE

131. EXPRESSION OF CANDIDATES AVRVG GENES DURING APPLE SCAB INFECTION

132. PRODUCTION OF TRANSGENIC PEAR PLANTS EXPRESSING FERRITIN GENE WITH THE AIM TO REDUCE FIRE BLIGHT SUSCEPTIBILITY

S. Djennane | C. Cesbron | S. Sourice | K. Loridon | E. Chevreau

133. EVALUATION OF THE RESISTANCE TO THE ROSY APPLE APHID USING A GENETIC MARKER

E. Dapena | M. Miñarro | M.D. Blázquez

134. GENETIC CHARACTERIZATION AND MAPPING OF THE MAJOR POWDERY MILDEW RESISTANCE GENE PLBJ FROM MALUS BACCATA JACKII

F. Dunemann | M. Schuster

135. GENETIC ANALYSIS OF IRON CHLOROSIS TOLERANCE IN MYROBALAN PLUM X ALMOND-PEACH HYBRIDS

M.J. Gonzalo | E. Dirlewanger | F. Laigret | M.A. Moreno | Y. Gogorcena

136. SOURCES OF WINTER HARDINESS IN SWEET CHERRY BREEDING IN ESTONIA

K. Kask | H. Jänes | P. Ardel

137. NEW BELARUSIAN APPLE CULTIVARS WITH DURABLE RESISTANCE TO SCAB

Z.A. Kazlouskaya

138. EVALUATION OF APPLE PLANTS FOR WINTER RESISTANCE BY PEROXIDASE ISOENZYME ANALYSIS

Z.A. Kazlouskaya | E.N. Biruk

139. RATIO OF HOMOZYGOUS AND HETEROZYGOUS VF GENOTYPES IN THE PROGENIES OF APPLE VFVF X VFVF CROSSES

140. RESPONSE OF LOCAL AND SCAB-RESISTANT CULTIVARS TO WOOLLY APPLE APHID

M. Miñarro | E. Dapena

141. SUSCEPTIBILITY OF ASIAN PEAR CULTIVARS AND ‘CONFERENCE’ PEAR TO APPLE BLOSSOM WEEVIL (ANTHONOMUS POMORUM L.)

E. Molenda | E. Pitera | A. Mikuła

142. EVALUATION OF A GERMPLASM COLLECTION OF SOME FRUIT TREE VARIETIES CONCERNING GENETIC RESISTANCE TO ABIOTIC FACTORS

143. AN OPTIMIZED CDNA-AFLP PROTOCOL FOR THE IDENTIFICATION OF TDFS INVOLVED IN THE MALUS-VENTURIA INAEQUALIS INTERACTION

144. RESPONSE OF PEAR GENOTYPES TO PSYLLA SP. ATTACK IN CENTRAL TRANSYLVANIA, ROMANIA

R. Sestras | C. Botez | M. Ardelean | I. Oltean | A. Sestras

145. MALUS TAXA, SELECTED IN HUNGARY, AS NEW GENE SOURCES OF RESISTANCE TO APPLE SCAB (VENTURIA INAEQUALIS CKE./WINT.)

M. Tóth | J. Halász | A. Pedryc | S. Kovács

146. ROSE HIP FLY (RHAGOLETIS ALTERNATA FALLEN) AND LEAF SPOT FUNGUS (SPHACELOMA ROSARUM (PASS.) JENKINS) – POSSIBLE THREATS AGAINST ROSE HIP PRODUCTION?

M. Uggla | B.U. Carlson-Nilsson

147. REVIEW OF POME FRUIT BREEDING IN EUROPE: WHICH STRATEGIES FOR THE NEAR FUTURE?

Y. Lespinasse