Home > IV International Symposium on Cucurbits > DEVELOPMENT AND APPLICATION OF MELON RESISTANCE GENE ANALOG (MRGA) MARKERS FOR DETECTION OF VIRUS RESISTANCE GENE IN MELON

Articles

DEVELOPMENT AND APPLICATION OF MELON RESISTANCE GENE ANALOG (MRGA) MARKERS FOR DETECTION OF VIRUS RESISTANCE GENE IN MELON

Article number

871_31

Pages

237 – 244

Language

English

Abstract

Creb-2, a gene for resistance to an Indonesia isolate of Cucumber mosaic virus (CMV-B2) in melon cultivar Yamatouri has previously been described as a single dominant gene.
Eleven melon resistance gene analogs (MRGAs) were initially isolated to elucidate the resistance mechanism conferred by Creb-2. Among 11 independent clones, MRGA1 showed a distinct polymorphism when used as a probe for restriction fragment length polymorphism (RFLP) analysis against Yamatouri and Vakharman for the Creb-2. MRGA1 was then developed and two set of sequence-tagged site (STS) primers (MRGA-f1 and MRGA-21) were designed.
However, only MRGA-f1 primers produced a polymorphism between Yamatouri and Vakharman, while MRGA-21 failed to obtain polymorphism.
The MRGA-f1 was applied for linkage map of Creb-2 in 104 F₂ progeny.
Linkage analysis showed that genetic distance of MRGH-f1₄₃₈ to the Creb-2 was 0.96 cM. MRGA-f1438 marker could be used for screening of CMV-B2 resistant cultivar in F₂ populations and makuwa melons.

Publication

IV International Symposium on Cucurbits

Authors

B.S. Daryono

Keywords

Cucumis melo L., CMV, Creb-2, resistance gene analogs

Full text

https://doi.org/10.17660/ActaHortic.2010.871.31

Online Articles (92)

1. CUCURBITS, WATERMELON, AND BENEFITS TO HUMAN HEALTH

P. Perkins-Veazie

2. CONSTRUCTION OF GENETIC LINKAGE MAP AND LOCALIZATION OF QTLS FOR POWDERY MILDEW RESISTANCE IN CUCUMBER (CUCUMIS SATIVUS L.)

3. BREEDING OF A NEW WATERMELON VARIETY ‘SEEDLESS ICE CREAM’

4. EST-SSR MARKER LINKED TO CUCUMBER TARGET LEAF SPOT RESISTANCE-RELATED GENE

Huizhe Wang | Shuju Li | Ruihuan Yang | Wei Guan

5. PRELIMINARY STUDY ON MAPPING OF GENES CONTROLLING STAMINATE FLOWER EXPRESSION AND QTL ANALYSIS OF THE RATIO OF PISTILLATE FLOWERS IN MELON (CUCUMIS MELO L.)

Yunyan Sheng | Xuqiang Lu | Xuezheng Wang | Feishi Luan

6. EFFECTS OF ROOTSTOCK ON FRUIT YIELD AND QUALITY OF HYDROPONICALLY CULTIVATED GRAFTED CUCUMBER UNDER NACL STRESS

7. AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS AND AGRONOMIC TRAITS ANALYSIS PROVIDE STRATEGIES FOR IMPROVEMENT OF BITTER GOURD (MOMORDICA CHARANTIA L.)

Yan Yang | Yuanfeng Zhan | Weixia Liu | Jihua Sun

8. GENETIC RELATIONSHIPS OF EDIBLE SEED WATERMELONS ON PHENOTYPIC CHARACTERISTICS AND RAPD ANALYSIS

Jiannong Zhang | Guifen Zhang

9. GENETIC ANALYSIS AND THE SSR-BASED GENETIC LINKAGE MAP OF BLUNTED WITH BLOSSOM TRAIT IN CUCUMIS SATIVUS L.

Ming Xin | Zhiwei Qin

10. INHERITANCE OF PUBESCENCE TYPES IN MELON (CUCUMIS MELO L.): HISPID, GLABROUS AND PUBERULENT

Huilin Wang | Wenyan Zhao | Depei Lin

11. GENETIC MODEL ANALYSIS OF THE CHARACTERISTIC AROMATIC SUBSTANCE 2E, 6Z-NONADIENAL IN CUCUMBER

Qiang Xu | Youling Geng | Xiaohua Qi | Xuehao Chen

12. COMPARISON OF ADAPTABILITY OF MUSKMELON CULTIVATED IN OPEN FIELD OF ECOLOGICAL REGION OF OASIS ON THE SOUTHERN MARGIN OF MU US DESERT

H.L. Li | ZH.F. Chen | M. Jing

13. GENETIC ANALYSIS ON NODE RATES OF MULTI-PISTILLATE FLOWERS IN CUCUMBER

Xinxin Dou | Xiaoyan Zou | Xixiang Li | Di Shen | Haiping Wang

14. EFFECT OF HYDRO-PRIMING ON SEED GERMINATION OF TRIPLOID WATERMELON [CITRULLUS LANATUS (THUNB) MATSUM &AMP; NAKAI]

Guilan Gu | Qing Xu | Xian Zhang | Qianqian Liang

15. GENETIC STUDY ON SUGAR AND SOUR TRAITS OF MELON (CUCUMIS MELO L.)

16. CHARACTERIZATION OF GENETIC DIVERSITY IN CUCURBITA MOSCHATA

Xinzheng Li | Xiaohua Du | Yongdong Sun | Junguo Zhou | Runzhou Zhao

17. IDENTIFICATION OF A RESOURCE OF POWDERY MILDEW RESISTANCE IN CUCURBITA MOSCHATA

Junguo Zhou | Huiling Hu | Xinzheng Li | Ruijin Zhou | Huirong Zhang

18. SCREENING OF FIFTEEN PUMPKIN (CUCURBITA MOSCHATA) GENOTYPES FOR YIELD AND SOME QUALITY UNDER SOHAG CONDITIONS

H.A. Obiadalla-Ali | K.A.A. El Shaikh | N.A. El Sawah | A.A. Helaly

19. DEVELOPING NEW PROMISING GALIA MELON F₁ HYBRIDS BY UTILIZING SOME EGYPTIAN MELON GENETIC RESOURCES

A.A. Glala | S.A. Saleh | O.M. Sawaan | N.M. Omar

20. CHROMOSOME CHARACTERIZATION OF CELEBES LOCAL CUCUMBER (CUCUMIS SP.)

B.S. Daryono | D.A. Kumalawati | S. Abdullah

21. SEARCH FOR QUANTITATIVE INDICATORS OF FRUIT TEXTURE FOR BREEDING IN CUCUMBER

Y. Yoshioka | H. Horie | M. Sugiyama | Y. Sakata | Y. Tamaki

22. CONSTRUCTION OF A GENETIC LINKAGE MAP AND IDENTIFICATION OF A MAJOR QTL CONFERRING RESISTANCE TO POWDERY MILDEW IN THE MELON GENOTYPE TGR-1551

23. CHARACTERIZATION OF SOME MELON GENOTYPES COLLECTED FROM EASTERN AND CENTRAL ANATOLIA REGION OF TURKEY

24. INHERITANCE AND MOLECULAR MAPPING OF ANDROMONOECIOUS AND GYNOECIOUS SEX DETERMINING GENES IN MELON (CUCUMIS MELO L.)

Hongyan Ma | Feishi Luan | Yunyan Sheng | Meiling Gao

25. MELON BREEDING FOR RESISTANCE TO FUSARIUM WILT USING MOLECULAR MARKERS AND DIHAPLOIDIZATION

E. Ekbic | M. Yildiz | G. Baktemur | S. Buyukalaca | K. Abak

26. THE BREEDING OF A NEW PUMPKIN CULTIVAR ‘HONGLI NO 2’

27. VARIATION FOR MORPHOLOGICAL TRAITS WITHIN AND AMONG CUCURBITA PEPO GENOTYPES

A. Lebeda | E. Křístková | H.S. Paris

28. PROGRESS IN BREEDING OF EARLY MATURE, THICK RIND MELON IN XINJIANG

29. EVALUATION ON CRACK RESISTANCE OF WATERMELON (CITRULLUS LANATUS)

30. SELECTION OF LOCAL AND EXOTIC CUCUMBER CULTIVARS UNDER PLASTIC HOUSE ENVIRONMENT IN THE HIGH HILL OF NEPAL

K.P. Upadhyay

31. DEVELOPMENT AND APPLICATION OF MELON RESISTANCE GENE ANALOG (MRGA) MARKERS FOR DETECTION OF VIRUS RESISTANCE GENE IN MELON

B.S. Daryono

32. PRELIMINARY STUDY OF RESISTANCE AGAINST BEMISIA TABACI GENN. IN TGR-1551 MELON GENOTYPE

F.J. Palomares-Rius | A.I. López-Sesé | M.L. Gómez-Guillamón

33. DISTRIBUTION AND HARMFULNESS OF PSEUDOPERONOSPORA CUBENSIS ON CUCURBITS IN THE CZECH REPUBLIC IN 2005-2007

A. Lebeda | J. Hübschová

34. EFFECTS OF DROUGHT ON YIELD AND POMOLOGICAL FEATURES OF WILD AND DOMESTIC TURKISH WATERMELON GENOTYPES

M. Zeki Karipcin | N. Sari | H. Kirnak

35. NEW FUSARIUM WILT RESISTANT MELON (CUCUMIS MELO VAR. CANTALUPENSIS) VARIETIES DEVELOPED BY DIHAPLOIDIZATION: SARI F₁, YETISIR F₁, SOLMAZ F₁, EMIN F₁ AND YUCEL F₁

N. Sari | I. Solmaz | H. Yetisir | H. Ekiz | S. Yucel

36. QUALITY SEED PRODUCTION OF PARENTAL LINE OF PUMPKIN (CUCURBITS MOSCHATA L.) UNDER INSECT PROOF NET HOUSE

B. Singh | B.S. Tomar | S. Thakur

37. PLUG-TRAY NURSERY RAISING TECHNOLOGY FOR OFF-SEASON CUCURBITS CULTIVATION

B. Singh | B.S. Tomar | M. Hasan

38. EFFECT OF PHOTOPERIOD ON VEGETATIVE TRAITS, FEMALE FLOWER NUMBER AND STOMATAL CONDUCTIVITY OF IRANIAN FLEXUOSUS AND DUDAIM GENOTYPES

F. Soltani | A. Kashi | Z. Zamani | Y. Mostofi

39. RELATIONSHIP BETWEEN DROUGHT TOLERANCE AND STOMATA DENSITY IN MELON

S. Kusvuran | H.Y. Dasgan | S. Kuçukkomurcu | K. Abak

40. EFFECT OF VESICULAR ARBUSCULAR MYCORRHIZAS ON PLANT GROWTH OF SOILLESS GROWN MUSKMELON

K. Abak | H.Y. Dasgan | Y. Rehber | I. Ortaş

41. EFFECTS OF NACL AND ISO-OSMOTIC PEG STRESS ON THE GROWTH AND OSMOTIC COMPONENTS OF CUCUMBER SEEDLING

Liang Chen | Yuan Huang | Ai Zhen | Zhilong Bie

42. CHANGES IN CARBOHYDRATES AND CARBOHYDRATE METABOLIZING ENZYMES DURING WATERMELON FRUIT SET

Lanchun Nie | Jifang Cui | Xueying Zhang | Bao Di

43. PHOTOSYNTHETIC ABILITY AND MINERAL CONCENTRATIONS IN XYLEM EXUDATE OF GRAFTED AND NON-GRAFTED WATERMELON SEEDLINGS

Lanchun Nie | Haiyuan Chen | Xueying Zhang | Bao Di

44. EFFECTS OF CHILLING TEMPERATURES ON CELLULARITY AND ULTRASTRUCTURE OF HONEY DEW FRUITS

Fang Liu | Yuxin Zhang | Chunlin Wang | Nianlai Chen

45. PHOTOSYNTHETIC AND BIOCHEMICAL CHANGES OF MELON LEAVES DURING SENESCENCE

Nianlai Chen | Ruiyong Tang | Yuxin Zhang | Cuixiang An | Huijuan Gao

46. PHYSIOLOGICAL RESPONSES OF TWO CUCURBITA SPP. ROOTSTOCKS TO NA₂CO₃ STRESS

Yongdong Sun | Xinzheng Li | Xiaohua Du | Anguo Qi | Junguo Zhou

47. IDENTIFICATION OF COLD-TOLERANCE BY CHANGING OF DIFFERENCE IN LEAF-AIR TEMPERATURE OF CUCURBITA PEPO L.

Lei Fengjin | Wen Xiangzhen | Li Yaling | Li Yan | Li Xiaoqi

48. THE EFFECT OF TEMPERATURE AND LIGHT DENSITY ON PLANT GROWTH, PHYSIOLOGICAL CHARACTERISTICS OF CHIEH-QUA SEEDLING

Shiwei Song | Hongxing Huang | Houcheng Liu | Guangwen Sun | Riyuan Chen

49. DIFFERENCE IN PHOTOSYNTHESIS OF DIFFERENT POSITION LEAVES OF GREENHOUSE CUCUMBER IN AUTUMN AND WINTER

X.T. Ding | H.J. Jin | H.M. Zhang | J.Z Yu

50. INFLUENCES OF GRAFTING ON AROMATIC COMPOUNDS IN MUSKMELON BY SOLID PHASE MICROEXTRACTION WITH GC-MS

Shouhua Xiao | Shancang Zhao | Chongqi Wang | Yumei Dong | Zigao Jiao

51. EFFECT OF CL^– STRESS ON PHOTOSYNTHETIC, CHLOROPHYLL FLUORESCENCE CHARACTERS AND CHLOROPLAST ULTRA-STRUCTURE OF WATERMELON SEEDLINGS

Zhaohui Zhang | Liping Wang | Shuping Huang | Lidong Xing | Lin Wang

52. EFFECT OF DIFFERENT LEVELS OF SHADING ON YIELD AND FRUIT QUALITY OF CUCUMBER (CUCUMIS SATIVUS)

M. Naraghi | M. Lotfi

53. WATERMELON (CITRULLUS LANATUS) GRAFTING METHOD TO REDUCE LABOR COST BY ELIMINATING ROOTSTOCK SIDE SHOOTS

F.D. Memmott | R.L. Hassell

54. SOWING DATE, PLANT DENSITY, FRUITS PER PLANT AND HARVEST TIME INFLUENCED YIELD AND QUALITY OF HYBRID SEED OF BITTER GOURD (MOMORDICA CHARANTIA)

55. EFFECTS OF BTH AND SA TREATMENT ON ASSIMILATE ALLOCATION, FRUIT YIELD AND QUALITY OF MELON

Nianlai Chen | Xi-e Li | Ming Hu | Yuxin Zhang

56. BREEDING POTENTIAL OF INDIAN GERMPLASM OF CUCUMIS SATIVUS VAR. HARDWICKII FOR CUCUMBER IMPROVEMENT

H. Choudhary | D.K. Singh

57. THE ACTIVITIES OF PEROXIDASE AND POLYPHENOLOXIDASE, AND THE CONTENT OF SOLUBLE PROTEIN IN FUSARIC ACID RESISTANT CHIEH-QUA DURING INFECTION WITH FUSARIUM OXYSPORUM

58. SYNTHETIC HUMIC ACID INCREASED NUTRIENT UPTAKE IN CUCUMBER SOILLESS CULTURE

J.A. Olfati | GH. Peyvast | R. Qamgosar | Z. Sheikhtaher | M. Salimi

59. EFFECT OF SUBSTRATE ON GREENHOUSE CUCUMBER PRODUCTION IN SOILLESS CULTURE

GH. Peyvast | J.A. Olfati | P. Ramezani Kharazi | O. Noori Roudsari

60. CHARACTERIZATION OF FUNGAL PATHOGENS OCCURRING IN POTTING MIXTURES AND ORGANIC FERTILIZERS IN OMAN

A.M. Al-Sadi | A.H. Al-Jabri | I.H. Al-Mahmooli | F.A. Al-Said | M.L. Deadman

61. CAN SILICON IMPROVE DEFENSE RESPONSES OF CUCUMBER SEEDLINGS TO PYTHIUM-INDUCED DAMPING OFF DISEASE?

A.M. Al Sadi | F.A. Al Said | M.L. Deadman | A. Drenth | E.A.B. Aitken

62. THE USE OF RHIZOTRONS TO STUDY THE INTERACTIONS BETWEEN CUCURBIT ROOTS AND SOIL-BORNE PATHOGENS

M.L. Deadman | A.M. Al Sadi | F. Al Said | S. Al Affani

63. SPATIAL ANALYSIS OF PHYLOGENETIC AND PHENOTYPIC DIVERSITY IN PYTHIUM POPULATIONS FROM A SINGLE GREENHOUSE

A.M. Al Sadi | A.G. Al Ghaithi | M.L. Deadman | F.A. Al Said

64. FUNGICIDE EFFECTIVENESS ON CZECH POPULATIONS OF PSEUDOPERONOSPORA CUBENSIS

J. Hübschová | A. Lebeda

65. VARIATION FOR FUNGICIDE RESISTANCE AMONG CUCURBIT POWDERY MILDEW POPULATIONS IN THE CZECH REPUBLIC

A. Lebeda | B. Sedláková | M. Pejchar | H. Jeřábková

66. OCCURRENCE OF AMPELOMYCES QUISQUALIS ON THE CUCURBIT POWDERY MILDEW SPECIES IN THE CZECH REPUBLIC

B. Sedláková | A. Lebeda

67. THE USE OF CUCURBIT HYBRID ROOTSTOCKS IN THE MANAGEMENT OF PYTHIUM-INDUCED DAMPING-OFF OF CUCUMBER SEEDLINGS

M.L. Deadman | A.M. Al Sadi | F.A. Al Said | Q. Al Maawali

68. UTILIZATION OF NOVEL GENOTYPE OF BOTTLE GOURD FOR IDENTIFICATION OF HYBRID SEED WITH RAPD MARKER

D.K. Singh | S. Padiyar | H. Choudhary | A. Tiwari | H.H. Ram

69. EVALUATING THE TRAITS RELATED WITH FRUIT FLAVOR IN CUCUMBER

E. Aliabadi | M. Lotfi | R. Amiri

70. STUDY ON CHROMIUM STORAGE IN DIFFERENT ORGANS OF PUMPKIN (C. MAXIMA DUCH)

Yongan Cheng | Jinyin Lu | De Yang | Zunlian Zhao

71. AROMA CHARACTERIZATION OF ‘FLAVOR NO. 3’ MELON USING HEADSPACE-SOLID PHASE MICROEXTRACTION COMBINED WITH GAS CHROMATOGRAPHY-MASS SPECTROMETRY

Mi Tang | Zhilong Bie | Mingzhu Wu | Hongping Yi | Jiongxin Feng

72. PRELIMINARY STUDIES ON CUCUMBER DOWNY MILDEW ISOLATES IN NORTHERN THAILAND

P. Charoenwattana

73. INTEGRATED APPROACHES FOR MANAGEMENT OF PHYTOPHTHORA BLIGHT (PHYTOPHTHORA CAPSICI) OF CUCURBITS

M. Babadoost | C. Pavon | S.B. Thruppoyil

74. POTENTIAL IMPACT OF A NEW HIGHLY VIRULENT RACE OF FUSARIUM OXYSPORUM F. SP. NIVEUM IN WATERMELON IN THE USA

X.G. Zhou | K.L. Everts | B.D. Bruton

75. LYCOPENE AND CITRULLINE CONTENTS IN WATERMELON (CITRULLUS LANATUS) FRUIT WITH DIFFERENT PLOIDY AND CHANGES DURING FRUIT DEVELOPMENT

76. DEVELOPMENT OF AN EMBRYOGENIC SUSPENSION CULTURE IN CUCUMIS MELO

H. Asad | M. Lotfi | K. Vahdati

77. STUDY ON THE EMBRYOGENESIS OF CROSSES OF TETRAPLOID WITH DIPLOID IN CUCUMBER

78. GENETIC VARIATION ANALYSIS OF THE NEWLY SYNTHESIZED ALLOTETRAPLOIDS CUCUMIS HYTIVUS USING RETROTRANSPOSON-BASED SSAP MARKERS

Biao Jiang | Qunfeng Lou | Jinfeng Chen

79. CHARACTERIZATION OF NBS-LRR RESISTANCE GENE ANALOGS FROM A HIGH RESISTANCE TO DOWNY MILDEW INTROGRESSION LINE FROM CUCUMIS HYSTRIX ×C. SATIVUS

Hongjian Wan | Jinfeng Chen

80. EFFECTS ON CARBOHYDRATE AND AMINO ACIDS ACCUMULATION IN MUSKMELON FRUIT (CUCUMIS MELO L.) WITH DIFFERENT GRAFTING ROOTSTOCKS

81. THE EFFECTS OF PROLINE AND COCONUT WATER ON CALLUS INDUCTION OF CUCUMBER (CUCUMIS SATIVUS L.)

P. Tantasawat | W. Chaowiset | A. Sorntip | C. Kativat | A. Wannajindaporn

82. LATEST ADVANCES IN WATERMELON GENOMICS

83. QTL ANALYSES OF ORANGE COLOR AND CAROTENOID CONTENT AND MAPPING OF CAROTENOID BIOSYNTHESIS GENES IN CUCUMBER (CUCUMIS SATIVUS L.)

Hui Song | Jinfeng Chen | J. Staub | P. Simon

84. PRIMING EFFECT ON CUCUMBER GERMINATION AT LOW TEMPERATURES

GH. Peyvast | J.A. Olfati | M. Piri | M.B. Mahdieh

85. STUDIES ON PRIORITY OF MELON FRUITS TRAITS ASSOCIATED WITH EGYPTIAN CONSUMER PREFERENCE

A.A. Glala | E.H. Abd El-Samad | S.M. Adam | Y.I. Helmy

86. A STUDY ON THE RELATIONSHIP BETWEEN CUCUMBER GYNOGENESIS AND CONTENT OF OVARY HORMONES AND POLYAMINES

Aimin Wei | Shengli Du | Yike Han | Nan Liu | Guihua Zhang

87. ESTABLISHMENT OF CUCURBITA MAXIMA GENETIC TRANSFORMATION SYSTEM BY AGROBACTERIUM-MEDIATED METHOD

HongBing Fu | ShuPing Qu | ChongShi Cui | ZhuGang Li | Xi Zhao

88. EFFECT OF DIFFERENT POLLINATION DATES WITH IRRADIATED POLLENS ON FRUIT SET, HAPLOID EMBRYO INDUCTION AND PLANT OBTENTION IN TURKISH (KIRKAGAC, YUVA AND HASANBEY) MELONS

89. THE DIFFERENTIAL EXPRESSION OF RESISTANT GENES RELATED TO CUCUMBER SCAB DISPLAYED BY CDNA-AFLP

90. ISOLATION AND EXPRESSION PATTERN OF COR15B AND KIN1 GENES IN WATERMELON AND SQUASH

XiaoQian Zhu | GuoZhang Kang | ZhongHou Zhu | XueJie Zhu

91. STUDIES ON MITOTIC CHROMOSOME PREPARATION AND KARYOTYPE ANALYSIS OF TWO DIFFERENT GROUPS OF MELON IN CHINA

92. POLYMORPHISM ANALYSES OF WATERMELON (CITRULLUS LANATUS) MAPPING PARENTS USING RAPD AND ISSR MOLECULAR MARKERS

X.P. Yang | G. Liu | J.H. Xu | C.Z. Gao | X.L. Hou